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Porphyromonas gingivalis Induces RANKL in T-cells

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Abstract

Porphyromonas gingivalis is an oral pathogen highly implicated in chronic periodontitis, a disease characterized by inflammatory destruction of the tooth-supporting alveolar bone and eventually, tooth loss. T-cell innate immune responses are actively involved in this pathological process. Receptor activator of NF-κB Ligand (RANKL) is a cytokine that stimulates bone resorption, while its soluble decoy receptor osteoprotegerin (OPG) blocks its action. This study aimed to investigate in Jurkat T-cells the effects of P. gingivalis on the RANKL-OPG system and the major inflammatory mediator of bone resorption prostaglandin E2 (PGE2). P. gingivalis caused concentration-dependent up-regulation of RANKL gene expression and protein production, assessed by quantitative PCR and ELISA, respectively. PGE2 production was also enhanced. However, OPG was not detected. In conclusion, P. gingivalis induces RANKL and PGE2 in T-cells, potentially favoring bone resorption. These T-cell responses to P. gingivalis may contribute to the pathogenesis of inflammatory alveolar bone destruction occurring in chronic periodontitis.

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Acknowledgments

This study was supported by the authors’ institutes. The authors would like to thank Prof. Michael Curtis (The Blizard Institute of Cell and Molecular Science, Barts and the London School of Medicine and Dentistry) for providing the P. gingivalis strain.

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Authors and Affiliations

  1. Institute of Oral Biology, Center of Dental and Oral Medicine and Cranio-Maxillofacial Surgery, University of Zürich, Plattenstrasse 11, 8032, Zürich, Switzerland

    Georgios N. Belibasakis & Nagihan Bostanci

  2. Institute of Dentistry, Barts and the London Queen Mary’s School of Medicine and Dentistry, London, UK

    Durga Reddi

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  1. Georgios N. Belibasakis
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  2. Durga Reddi
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  3. Nagihan Bostanci
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Correspondence to Georgios N. Belibasakis.

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Belibasakis, G.N., Reddi, D. & Bostanci, N. Porphyromonas gingivalis Induces RANKL in T-cells. Inflammation 34, 133–138 (2011). https://doi.org/10.1007/s10753-010-9216-1

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