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Bacterial LPS Mediated Acute Inflammation-induced Spermatogenic Failure in Rats: Role of Stress Response Proteins and Mitochondrial Dysfunction

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Abstract

Bacterial Lipopolysaccharide (LPS) induced inflammation is implicated in the infection associated testicular tissue damage. Earlier, using a LPS induced acute endotoxemic rat model, we have shown the involvement of inflammation-induced oxidative stress in the impaired steroidogenesis and spermatogenesis. In the present study, we report a significant induction (more than 2-fold) of stress response proteins HSP-60, HMGB-1 and 2 in the testes, as early as 6 h after LPS injection with a later decrease. This induction of acute stress is closely followed by a significant reduction (74%) in Bcl2/Bax ratio along with leakage of cytochrome c (3 fold increase, p < 0.05) from mitochondria and increased caspase-3 activity levels (2.9 fold, p < 0.05) at 12 h and 24 h post LPS injection respectively. Further studies on PARP cleavage revealed a pattern similar to necrotic death during early periods (3 h to 24 h) and apoptosis at later periods (24 h to 72 h) after LPS treatment. In conclusion, the present study shows the involvement of stress response proteins and mitochondrial dysfunction in LPS-induced germ cell death in male rats.

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Acknowledgments

This work was supported by research grants from Council of Scientific and Industrial Research (CSIR) (Grant # 37(1221)/05/EMR-II), Govt. of India. The authors thank Council of Scientific and Industrial Research, New Delhi, India, for providing fellowship to Mr. Mallikarjuna Reddy Metukuri in the form of JRF and SRF. Authors acknowledge the help and support from Dr. GV Reddy and Mr. Ch Shiva kumar during this research study.

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Authors and Affiliations

  1. Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, AP, India, 500 046

    Mallikarjuna Reddy Metukuri, Chandra Mohan T. Reddy, P. R. K. Reddy & Pallu Reddanna

  2. Department of Medicine, E1114 Biomedical Sciences Tower, University of Pittsburgh, 200 Lothrop Street, Pittsburgh, PA, USA, 15213

    Mallikarjuna Reddy Metukuri

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  1. Mallikarjuna Reddy Metukuri
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  3. P. R. K. Reddy
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Correspondence to Pallu Reddanna.

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Metukuri, M.R., Reddy, C.M.T., Reddy, P.R.K. et al. Bacterial LPS Mediated Acute Inflammation-induced Spermatogenic Failure in Rats: Role of Stress Response Proteins and Mitochondrial Dysfunction. Inflammation 33, 235–243 (2010). https://doi.org/10.1007/s10753-009-9177-4

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