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The Anti-Inflammatory Effects of a Methanolic Extract from Radix Isatidis in Murine Macrophages and Mice

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Abstract

Radix Isatidis is the dried root of the plant Isatidis indigotica Fort (family Cruciferae) and traditionally used as an anti-viral, anti-bacterial, anti-endotoxic, and immune regulatory agent in the folk medicine of Korea and China. The aim of the present study was to determine the anti-inflammatory effects of methanolic extracts of Radix Isatidis (RIME) in lipopolysaccharide (LPS)-stimulated murine macrophages and in a TPA-induced ear edema animal model. Anti-inflammatory effects of RIME were examined in LPS-stimulated RAW 264.7 macrophages. In order to investigate the effects of RIME in vivo, activation of myeloperoxidase, and histological assessment were examined in the 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced mouse ear edema model. RIME significantly inhibited the release from macrophages of inflammatory mediators such as nitric oxide, prostaglandin E2, and pro-inflammatory cytokines. Topical administration of RIME at 1–5 mg/ear resulted in reduction of ear inflammation in mice. Thus, our results indicate that the anti-inflammatory effects of RIME involve decreased production of inflammatory mediators, which suggests that RIME may have therapeutic potential in a variety of inflammation-related diseases.

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ACKNOWLEDGMENTS

This work was supported by the Regional Innovation Center (RIC) program of the Ministry of Knowledge Economy, Republic of Korea and Korea Association of Small Business Innovation Research (S6070031210), Republic of Korea. The authors greatly appreciate Dr. Jae-In Park (Department of Animal products and Food Science, Kangwon National University, Republic of Korea) for preparation of RIME.

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Authors and Affiliations

  1. Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University, Chuncheon, 200-702, Republic of Korea

    Eun Kyung Shin, Dae Hwan Kim, Hyun-Kyung Shin & Jin-Kyung Kim

  2. AD Biotech Co., Ltd. 857-4, Toegye-Dong, Chuncheon, 200-170, Republic of Korea

    Hwan Lim

  3. Department of Food Chemistry and Nutrition, Hallym University, Chuncheon, 200-702, Republic of Korea

    Hyun-Kyung Shin

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  1. Eun Kyung Shin
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Correspondence to Jin-Kyung Kim.

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Shin, E.K., Kim, D.H., Lim, H. et al. The Anti-Inflammatory Effects of a Methanolic Extract from Radix Isatidis in Murine Macrophages and Mice. Inflammation 33, 110–118 (2010). https://doi.org/10.1007/s10753-009-9164-9

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