Abstract
Catecholamines are frequently used in sepsis, but their interaction with mitochondrial function is controversial. We incubated isolated native and endotoxin-exposed swine liver mitochondria with either dopamine, dobutamine, noradrenaline or placebo for 1 h. Mitochondrial State 3 and 4 respiration and their ratio (RCR) were determined for respiratory chain complexes I, II and IV. All catecholamines impaired glutamate-dependent RCR (p = 0.046), predominantly in native mitochondria. Endotoxin incubation alone induced a decrease in glutamate-dependent RCR compared to control samples (p = 0.002). We conclude that catecholamines and endotoxin impair the efficiency of mitochondrial complex I respiration in vitro.
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Supported by grant 3200BO/102268 from the Swiss National Fund.
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This work should be attributed to the Bern University Hospital and University of Bern, CH-3010 Bern, Switzerland
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Porta, F., Bracht, H., Weikert, C. et al. Effects of Endotoxin and Catecholamines on Hepatic Mitochondrial Respiration. Inflammation 32, 315–321 (2009). https://doi.org/10.1007/s10753-009-9138-y
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DOI: https://doi.org/10.1007/s10753-009-9138-y