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Hydrogen Peroxide in Exhaled Breath Condensate (EBC) vs Eosinophil Count in Induced Sputum (IS) in Parenchymal vs Airways Lung Diseases"

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Abstract

We compared exhaled breath condensate (EBC) and induced sputum (IS) for assessing inflammation in pulmonary diseases in patients with obstructive lung disease (n = 20), persistent cough >6 months (n = 20), interstitial lung disease (n = 25) and controls (n = 10). EBC was collected by suspending a Teflon perfluoroalkoxy tube installed in an ice-filled container and connected to a polypropylene test tube. IS was recovered after 20’ inhalation of 3% saline with an ultrasonic nebulizer, and 300 cells were differentially counted in cytospin Giemsa-stained slides. H202 was measured by a method based on oxidation of phenolsulfonphthalein (phenol red) mediated by horseradish peroxidases and H202. Pulmonary function tests were performed by conventional methods. H202 levels in EBC and % eosinophils in IS were significantly different between groups. A positive and significant correlation was found between % eosinophils in IS and the levels of H202 in EBC for each group and for all patients combined.

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Acknowledgment

Esther Eshkol is thanked for editorial assistance.

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Authors and Affiliations

  1. Institute of Pulmonary and Allergic Diseases, Tel-Aviv Sourasky Medical Center and National Laboratory Service for ILD, Sakler Faculty of Medicine, Tel-Aviv University, 6 Weizman Street, Tel-Aviv, 64239, Israel

    Elizabeth Fireman, Moshe Shtark, Ram Mor & Shmuel Kivity

  2. The Department of Pulmonary Medicine, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel

    Israel E. Priel

  3. The Edith Wolfson Medical Center, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel

    Israel E. Priel

  4. NHLI, Imperial College School of Medicine, London, UK

    Robert Shiner

  5. Gastroenterology Department, Hillel Yaffe Medical Center, Hadera, Israel

    Zvi Fireman

  6. Rappapot Faculty of Medicine, Technion, Haifa, Israel

    Zvi Fireman

  7. Institute of Pulmonary and Allergic Diseases, Tel-Aviv Sourasky Medical Center and Laboratory Service for ILD, 6 Weizman Street, Tel-Aviv, 64239, Israel

    Elizabeth Fireman

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  1. Elizabeth Fireman
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Correspondence to Elizabeth Fireman.

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Fireman, E., Shtark, M., Priel, I.E. et al. Hydrogen Peroxide in Exhaled Breath Condensate (EBC) vs Eosinophil Count in Induced Sputum (IS) in Parenchymal vs Airways Lung Diseases". Inflammation 30, 44–51 (2007). https://doi.org/10.1007/s10753-007-9020-8

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