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Acute exposure of larval rainbow trout (Oncorhynchus mykiss) to elevated temperature limits hsp70b expression and influences future thermotolerance

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Abstract

As poikilotherms, fish health is compromised by exposure to elevated temperatures (e.g. climate change-related warming, anthropogenic thermal pollution, and/or hatchery processes). While fish thermotolerance has been demonstrated to be plastic, the downstream impacts of early life-stage high temperature exposure are not known. In the present study, we investigated the thermotolerance of rainbow trout (Oncorhynchus mykiss) fry 2 months after being exposed to elevated temperature (22°C) for 96 h. Exposed fry demonstrated a reduced critical thermal maxima (CTmax) in comparison to control fish. Using the RNase H-dependent quantitative PCR method, expression of rainbow trout hsp70 isoforms was determined immediately after the acute thermal stress and immediately following the thermotolerance trials. The lowered CTmax was associated with a reduced ability to upregulate the hsp70b gene during the thermotolerance trials, whereas no changes in hsp70a were observed. Overall, these results indicate that exposure to thermal stress in early life-stages of rainbow trout can have negative effects on future physiological function.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgments

The authors would like to thank Greg Goss for the use of laboratory space and animals. They are grateful for Brian Charles and Raven Brood Trout Station for providing rainbow trout embryos. Thanks to Tad Plesowicz and Erik Folkerts for assistance with fish husbandry. The authors would also like to thank Troy Locke and the staff at MBSU for their support and expertise. This research was supported by a Campus Alberta Innovates Program Research Chair to CNG.

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  1. Faculty of Science and Technology and Athabasca River Basin Research Institute, Athabasca University, Athabasca, AB, Canada

    Salvatore D. Blair & Chris N. Glover

  2. Department of Biological Sciences, University of Alberta, Edmonton, Canada

    Salvatore D. Blair & Chris N. Glover

  3. Department of Biology, Winthrop University, 307 Dalton Hall, Rock Hill, SC, USA

    Salvatore D. Blair

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Blair, S.D., Glover, C.N. Acute exposure of larval rainbow trout (Oncorhynchus mykiss) to elevated temperature limits hsp70b expression and influences future thermotolerance. Hydrobiologia 836, 155–167 (2019). https://doi.org/10.1007/s10750-019-3948-1

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