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Seasonal patterns in stream periphyton fatty acids and community benthic algal composition in six high-quality headwater streams

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Abstract

Fatty acids are integral components of periphyton and differ among algal taxa. We examined seasonal patterns in periphyton fatty acids in six minimally disturbed headwater streams in Pennsylvania’s Appalachian Mountains, USA. Environmental data and periphyton were collected across four seasons for fatty acid and algal taxa content. Non-metric multidimensional scaling ordination suggested significant seasonal differences in fatty acids; an ordination on algal composition revealed similar seasonal patterns, but with slightly weaker separation of summer and fall. Summer and fall fatty acid profiles were driven by temperature, overstory cover, and conductivity and winter profiles by measures of stream size. Ordination on algal composition suggested that summer and fall communities were driven by overstory and temperature, whereas winter communities were driven by velocity. The physiologically important fatty acid 18:3ω6 was highest in summer and fall. Winter samples had the highest 20:3ω3. Six saturated fatty acids differed among the seasons. Periphyton fatty acids profiles appeared to reflect benthic algal species composition. This suggests that periphyton fatty acid composition can be useful in characterizing basal food resources and stream water quality.

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Acknowledgments

We thank Daniel Spooner and other members of the U.S. Geological Survey, Northern Appalachian Research Laboratory as well as Richard Mitchell of the U.S. Environmental Protection Agency, Barry Baldigo and Scott George, US Geological Survey for their insightful comments on earlier drafts of the manuscript. Comments from Zhengwen Liu and two anonymous reviewers also greatly improved the manuscript. Authors contributed equally in the work reported here. Use of trade, product or firm names does not imply endorsement by the U.S. Government.

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  1. U.S. Geological Survey, Northern Appalachian Research Laboratory, Leetown Science Center, Wellsboro, PA, 16901, USA

    Dale C. Honeyfield & Kelly O. Maloney

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  1. Dale C. Honeyfield
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  2. Kelly O. Maloney
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Correspondence to Dale C. Honeyfield.

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Handling editor: Zhengwen Liu

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Honeyfield, D.C., Maloney, K.O. Seasonal patterns in stream periphyton fatty acids and community benthic algal composition in six high-quality headwater streams. Hydrobiologia 744, 35–47 (2015). https://doi.org/10.1007/s10750-014-2054-7

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  • DOI: https://doi.org/10.1007/s10750-014-2054-7

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