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Behavioural Reproductive Isolation in a Rotifer Hybrid Zone

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Abstract

A hybrid zone between two Brachionus plicatilis rotifer mitochondrial DNA (mtDNA) lineages was recently described in the Iberian Peninsula between a pond (Santed 2) and a lake (Gallocanta). The patterns of mitochondrial and nuclear genetic variation observed suggested that gene flow is mainly male-mediated from the lake to the pond. Here we test two hypotheses: (a) that male-mediated gene flow occurs through assortative mating between individuals from these ponds, (b) that behavioural isolation occurs between the two mtDNA lineages. We isolated, reared and genotyped rotifer clones from resting eggs collected in the sediments of these and two other distant ponds. We devised a quick, inexpensive RFLP method to discriminate between B. plicatilis and its sibling species B. ‘Manjavaeas’ and between both mtDNA B. plicatilis lineages. Behavioural no-choice tests using new-born, virgin males and females were performed between five clones. B. ‘Manjavacas’ and B. plicatilis were reproductively isolated. B. plicatilis clones did not show evidence of reproductive isolation, regardless of their mtDNA lineage, except Santed 2 males, which discriminated strongly against Gallocanta females. These results could help to explain the discrepancies between mitochondrial and nuclear genetic variation reported in the two populations.

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References

  • J. Ciros-Pérez A. Gómez M. Serra (2001) ArticleTitleOn the taxonomy of three sympatric sibling species of the Brachionus plicatilis (Rotifera) complex from Spain, with the description of B. ibericus n. sp Journal of Plankton Research 23 1311–1328 Occurrence Handle10.1093/plankt/23.12.1311

    Article  Google Scholar 

  • L. Meester ParticleDe A. Gómez B. Okamura K. Schwenk (2002) ArticleTitleThe monopolization hypothesis and the dispersal-gene flow paradox in aquatic organisms Acta Oecologica 23 121–135 Occurrence Handle10.1016/S1146-609X(02)01145-1

    Article  Google Scholar 

  • O. Folmer M. Black W. Hoeh R. Lutz R. Vrijenhoek (1994) ArticleTitleDNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates Molecular Marine Biology and Biotechnology 3 294–299 Occurrence Handle7881515

    PubMed  Google Scholar 

  • Y. Fu A. Hagiwara K. Hirayama (1993) ArticleTitleCrossing between seven strains of the rotifer Brachionus plicatilis Nippon Suisan Gakkaishi 59 2009–2016

    Google Scholar 

  • A. Gómez G. J. Adcock D. H. Lunt G. R. Carvalho (2002a) ArticleTitleThe interplay between colonisation history and gene flow in passively dispersing zooplankton: microsatellite analysis of rotifer resting egg banks Journal of Evolutionary Biology 15 158–171 Occurrence Handle10.1046/j.1420-9101.2002.00368.x

    Article  Google Scholar 

  • A. Gómez G. R. Carvalho D. H. Lunt (2000) ArticleTitlePhylogeography and regional endemism of a passively dispersing zooplankter: mitochondrial DNA variation in rotifer resting egg banks Proceedings of the Royal Society of London Series B-Biological Sciences 267 2189–2197 Occurrence Handle10.1098/rspb.2000.1268

    Article  Google Scholar 

  • A. Gómez M. Serra (1995) ArticleTitleBehavioral reproductive isolation among sympatric strains of Brachionus plicatilis Muller 1786: insights into the status of this taxonomic species Hydrobiologia 313 111–119 Occurrence Handle10.1007/BF00025938

    Article  Google Scholar 

  • A. Gómez M. Serra (1996) ArticleTitleMate choice in male Brachionus plicatilis rotifers Functional Ecology 10 681–687

    Google Scholar 

  • A. Gómez M. Serra G. R. Carvalho D. H. Lunt (2002b) ArticleTitleSpeciation in ancient cryptic species complexes: Evidence from the molecular phylogeny of Brachionus plicatilis (Rotifera) Evolution 56 1431–1444

    Google Scholar 

  • A. Gómez T. W. Snell (1996) ArticleTitleSibling species and cryptic speciation in the Brachionus plicatilis species complex (Roti- fera) Journal of Evolutionary Biology 9 953–964 Occurrence Handle10.1046/j.1420-9101.1996.9060953.x

    Article  Google Scholar 

  • R. R. L. Guillard J. H. Ryther (1962) ArticleTitleStudies of marine planktonic diatoms I. Cyclotella nana Hustedt and Detonula confervacea (Cleve) Canadian Journal of Microbiology 8 229–239 Occurrence Handle13902807

    PubMed  Google Scholar 

  • T. Kotani A. Hagiwara (1999) ArticleTitleMate recognition of the rotifer Brachionus plicatilis Muller at different temperatures Bulletin of the Faculty of Fisheries of Nagasaki university 80 55–59

    Google Scholar 

  • T. Kotani A. Hagiwara (2003) ArticleTitleFertilisation between the rotifer Brachionus plicatilis strains at different temperatures Fisheries Science 69 1076–1078 Occurrence Handle10.1046/j.1444-2906.2003.00730.x

    Article  Google Scholar 

  • R. Ortells T. W. Snell A. Gómez M. Serra (2000) ArticleTitlePatterns of genetic differentiation in resting egg banks of a rotifer species complex in Spain Archiv für Hydrobiologie 149 529–551

    Google Scholar 

  • R. Rico-Martinez T. W. Snell (1995a) ArticleTitleMating behavior and mate recognition pheromone blocking of male receptors in Brachionus plicatilis Muller (Rotifera) Hydrobiologia 313 105–110 Occurrence Handle10.1007/BF00025937

    Article  Google Scholar 

  • R. Rico-Martinez T. W. Snell (1995b) ArticleTitleMale discrimination of female Brachionus plicatilis Muller and Brachionus rotundiformis Tschugunoff (Rotifera) Journal of Experimental Marine Biology and Ecology 190 39–49 Occurrence Handle10.1016/0022-0981(95)00030-U

    Article  Google Scholar 

  • K. Schwenk D. Posada P. D. N. Hebert (2000) ArticleTitleMolecular systematics of European Hyalodaphnia: the role of contemporary hybridization in ancient species Proceedings of the Royal Society of London Series B-Biological Sciences 267 1833–1842 Occurrence Handle10.1098/rspb.2000.1218

    Article  Google Scholar 

  • T. W Snell M. Childress (1987) ArticleTitleAging and loss of fertility in male and female Brachionus plicatilis (Rotifera) International Journal of Invertebrate Reproduction and Development 12 103–110

    Google Scholar 

  • T. W. Snell C. A. Hawkinson (1983) ArticleTitleBehavioral reproductive isolation among populations of the rotifer Brachionus plicatilis Evolution 37 1294–1305

    Google Scholar 

  • T. W. Snell F. H. Hoff (1987) ArticleTitleFertilization and male-fertility in the rotifer Brachionus plicatilis Hydrobiologia 147 329–334 Occurrence Handle10.1007/BF00025762

    Article  Google Scholar 

  • R. R. Sokal F. J. Rohlf (1995) Biometry W.H. Freeman and Co. New York 776

    Google Scholar 

  • Zar, J. H., 1984. Biostatistical Analyses. Prentice-Hall International, 718 pp

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Authors and Affiliations

  1. Department of Biological Sciences, University of Hull, HU6 7RX, Hull, UK

    Helen K. Berrieman, David H. Lunt & Africa Gómez

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  1. Helen K. Berrieman
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  2. David H. Lunt
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  3. Africa Gómez
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Correspondence to Africa Gómez.

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Berrieman, H.K., Lunt, D.H. & Gómez, A. Behavioural Reproductive Isolation in a Rotifer Hybrid Zone. Hydrobiologia 546, 125–134 (2005). https://doi.org/10.1007/s10750-005-4109-2

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