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The Influence of Nitrogen Nutrition on the Carbohydrate and Nitrogen Status of Emergent Macrophyte Acorus calamus L.

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Abstract

Carbon and nitrogen balance in Acorus calamus, a wetland species colonising littoral zones with a high trophic status, was studied under experimental conditions using water or sand culture with a defined composition of the nutrient solution. Influence of graded level of N (1.86, 7.5 and 18.6 mM) and/or forms of N (NH +4 versus NO –3 ) on the content of non-structural carbohydrates, free amino acids, total C, and total N was studied in Acorus rhizomes and roots to find possible connection with a reduced growth of Acorus plants under high N and NH +4 –N nutrition described in our previous study [Vojtíšková et al., 2004. Hydrobiologia 518: 9–22]. High N availability and pure NH +4 –N nutrition affected the C/N balance of rhizome and root systems of Acorus in a similar way. NH +4 –N was the only form of N elevated under the high N treatment. The major proportion of the total non-structural carbohydrates (TNC) was starch (91–93% and 51–64% in rhizomes and roots, respectively). The content of starch was significantly and and negatively affected by high N availability (P = 0.001), as well as by NH +4 –N nutrition (P=0.001). Amounts of simple soluble carbohydrates (sucrose, glucose, and fructose) were negligible in comparison to starch in rhizomes and branched roots (up to 5% of TNC), while roots without developed lateral roots (unbranched) contained up to 33% of TNC in the form of simple soluble sugars. Moreover, high hexoses/sucrose ratio, low starch/soluble sugars ratio, high content of N, and low C/N ratio support the notion that unbranched roots are metabolically active young roots with tissue differentiation in progress. A high content of free amino acids, typically with dominance of N-rich amino acids (Arg-46%, Gln-8%, Asn-7%), was found simultaneously with a low carbohydrate content under high N supply, which indicates that NH +4 received is effectively incorporated into the organic form by this species. Since the decrease in carbohydrate content was not accompanied by luxurious growth, other possible carbon consuming processes were discussed in relation to NH +4 nutrition. More dramatic changes in total N than C were found under high N availability resulting a shift in C/N ratio in favour of N. Although the shift towards N metabolism was obvious, no serious carbohydrate depletion occurred, which could explain the reduced growth of Acorus plants under high N and sole NH +4 –N nutrition described previously.

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Authors and Affiliations

  1. Department of Plant Physiology, Faculty of Science, Charles University in Prague, Viničná 5, 12844, Prague 2, Czech Republic

    Lenka Vojtíšková, Edita Munzarová, Olga Votrubová & Helena Lipavská

  2. Institute of Systems Biology and Ecology, Academy of Sciences of the Czech Republic, Dukelská 145, 37901, Třeboň, Czech Republic 8

    Hana Čížková

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  1. Lenka Vojtíšková
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  2. Edita Munzarová
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  3. Olga Votrubová
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  4. Hana Čížková
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  5. Helena Lipavská
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Correspondence to Lenka Vojtíšková.

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Vojtíšková, L., Munzarová, E., Votrubová, O. et al. The Influence of Nitrogen Nutrition on the Carbohydrate and Nitrogen Status of Emergent Macrophyte Acorus calamus L.. Hydrobiologia 563, 73–85 (2006). https://doi.org/10.1007/s10750-005-0929-3

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  • DOI: https://doi.org/10.1007/s10750-005-0929-3

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