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Up-regulation of SGTB is associated with neuronal apoptosis after neuroinflammation induced by lipopolysaccharide

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Abstract

SGTB (Small glutamine-rich tetratricopeptide repeat (TPR)-containing, β) plays a critical role in protein–protein interactions. The interaction between SGTB and heat shock cognate protein (Hsc70)/heat shock protein (Hsp70) has aroused much attention in recent years. The present study was designed to elucidate dynamic changes in SGTB expression and distribution in the cerebral cortex in a lipopolysaccharide (LPS)-induced neuroinflammation rat model. It was found that SGTB expression was increased significantly in apoptotic neurons after LPS injection. The result of our in vitro study suggested that SGTB up-regulation might be associated with neuronal apoptosis after H2O2 challenge. In addition, silencing of SGTB in cultured PC12 (Pheochromocytoma) by siRNA indicated that SGTB was required for neuronal apoptosis induced by oxidative stress. Our finding about the cellular signal pathway may provide a new strategy against neuronal apoptosis in neuroinflammation in CNS.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 21077061 and 81202368) and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China

    Maohong Cao, Wei Xu, Heyi Zheng, Xiang Tan, Lei Li, Ying Rui, Guangfei Xu, Gang Cui, Jian Xu, Jianhua Cao, Tao Tao, Kaifu Ke & Qiyun Wu

  2. Department of Orthopedics, Jinling Hospital, Nanjing, 210000, Jiangsu, China

    Jian Yu

  3. Department of Neurology, Affiliated Hospital of Soochow University, Soochow, 215000, Jiangsu, China

    Gang Cui

Authors
  1. Maohong Cao
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  2. Wei Xu
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  3. Jian Yu
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  4. Heyi Zheng
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  5. Xiang Tan
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  6. Lei Li
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  7. Ying Rui
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  8. Guangfei Xu
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  9. Gang Cui
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  10. Jian Xu
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  11. Jianhua Cao
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  12. Tao Tao
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  13. Kaifu Ke
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  14. Qiyun Wu
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Corresponding authors

Correspondence to Kaifu Ke or Qiyun Wu.

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Maohong Cao, Wei Xu and Jian Yu contributed equally to this work.

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Cao, M., Xu, W., Yu, J. et al. Up-regulation of SGTB is associated with neuronal apoptosis after neuroinflammation induced by lipopolysaccharide. J Mol Hist 44, 507–518 (2013). https://doi.org/10.1007/s10735-013-9517-4

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  • DOI: https://doi.org/10.1007/s10735-013-9517-4

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