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The detection of EBP50 expression using quantum dot immunohistochemistry in pancreatic cancer tissue and down-regulated EBP50 effect on PC-2 cells

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Abstract

Ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) is a putative tumor suppressor that is correlated with many human cancers. However, the function of EBP50 in pancreatic cancer (PC) has not been described. In this paper, the EBP50 expression level in PC tissues was characterized. In vitro, the effects of EBP50 down-regulation by siRNA in PC-2 and MiaPaCa-2 cells were evaluated. In addition, possible mechanisms that mediate the influence of EBP50 were examined. Our results show that the EBP50 expression pattern changes during transformation as there is a loss of the normal apical membrane distribution and an ectopic cytoplasmic over-expression of EBP50; furthermore, the EBP50 expression level is subsequently decreased during malignant progression. Down-regulation of EBP50 promoted cancer cell proliferation, increased the colony-forming ability of cells and accelerated the G1-to-S progression. Additionally, the loss of EBP50 accentuated β-catenin activity, increased cyclin E and phosphorylated Rb expression, and attenuated p27 expression compared to control cells. Our results suggest that EBP50 may function as a potential tumor suppressor.

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Acknowledgments

This research was supported by the Fundamental Research Funds from the Central Universities (No. 20103020101000194). The authors thank Mr. Hong Xia for providing technical assistance.

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Gastroenterology, Renmin Hospital of Wuhan University, No. 238 Jiefang Road, Wuhan, 430060, Hubei, China

    Meng-Yao Ji, Xiao-Guang Lv, Xiu-Lan Peng, Xiao-Fei Lei & Wei-Guo Dong

  2. Department of Cardiac Surgery, Center Hospital of Nanyang, Nanyang, Henan, China

    Di-kun Fan

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  1. Meng-Yao Ji
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  2. Di-kun Fan
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  3. Xiao-Guang Lv
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  4. Xiu-Lan Peng
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  5. Xiao-Fei Lei
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  6. Wei-Guo Dong
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Corresponding author

Correspondence to Wei-Guo Dong.

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Meng-Yao Ji and Di-kun Fan have contributed equally to this work.

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Ji, MY., Fan, Dk., Lv, XG. et al. The detection of EBP50 expression using quantum dot immunohistochemistry in pancreatic cancer tissue and down-regulated EBP50 effect on PC-2 cells. J Mol Hist 43, 517–526 (2012). https://doi.org/10.1007/s10735-012-9424-0

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  • DOI: https://doi.org/10.1007/s10735-012-9424-0

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