Abstract
To examine the proper margin for breast conservative surgery in Chinese women population. 40 breast cancer specimens were collected and each sample was dissected into several groups: primary tumor group, 1 cm paracarcinoma, 2 cm paracarcinoma, 3 cm paracarcinoma and excessive 3 cm paracarcinoma groups. The immunohistochemistry staining was performed to measure the expression levels of proliferating cell nuclear antigen (PCNA), c-erbB-2, p53, estrogen receptor (ER) and progesterone receptor (PR). The gene expressions of PCNA, c-erbB-2, and p53 gradually decreased with the increased distance from primary tumor (P < 0.05). The 1 and 2 cm paracarcinoma group (no differences between the two, P > 0.05) showed higher risk factors (c-erbB-2, p53) than the 3 cm and excessive 3 cm paracarcinoma groups (P < 0.05). The expression of PCNA, ER, and PR showed no correlation with cancer progression (P > 0.05). Beyond the paracarcinoma 2 cm distance, the tissues showed significant decreases in tumor gene expression, which could represent the appropriate region for breast conservative surgery.
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Kato T, Kameoka S, Kimura T, Nishikawa T, Kobayashi M (2002) C-erbB-2 and PCNA as prognostic indicators of long-term survival in breast cancer. Anticancer Res 22:1097–1103
Khoury MP, Bourdon JC (2011) p53 Isoforms: an intracellular microprocessor? Genes Cancer 2:453–465. doi:10.1177/1947601911408893
Kumar S, Walia V, Ray M, Elble RC (2007) p53 in breast cancer: mutation and countermeasures. Front Biosci 12:4168–4178
Law TT, Kwong A (2009) Surgical margins in breast conservation therapy: how much should we excise? Southern Med J 102:1234–1237. doi:10.1097/SMJ.0b013e3181bfd420
Lopez-Guerrero JA, Navarro S, Noguera R, Almenar S, Pellin A, Vazquez C, Llombart-Bosch A (2003) Histological tumor grade correlates with HER2/c-erB-2 status in invasive breast cancer: a comparative analysis between immunohistochemical (CB11 clone and Herceptest), FISH and differential PCR procedures. Arkh Patol 65:50–55
Luini A, Rososchansky J, Gatti G et al (2009) The surgical margin status after breast-conserving surgery: discussion of an open issue. Breast Cancer Res Treat 113:397–402. doi:10.1007/s10549-008-9929-0
Malkas LH, Herbert BS, Abdel-Aziz W et al (2006) A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker. Proc Natl Acad Sci USA 103:19472–19477. doi:10.1073/pnas.0604614103
McCann A, Johnston PA, Dervan PA, Gullick WJ, Carney DN (1989) c-erB-2 oncoprotein expression in malignant and nonmalignant breast tissue. Ir J Med Sci 158:137–140
Morrow M (2009) Breast conservation and negative margins: how much is enough? Breast 18(Suppl 3):S84–S86. doi:10.1016/S0960-9776(09)70279-6
Munhoz AM, Montag E, Arruda E et al (2009) Immediate reconstruction following breast-conserving surgery: management of the positive surgical margins and influence on secondary reconstruction. Breast 18:47–54. doi:10.1016/j.breast.2008.10.005
Royds JA, Iacopetta B (2006) p53 and disease: when the guardian angel fails. Cell Death Differ 13:1017–1026. doi:10.1038/sj.cdd.4401913
Yamashiro N, Tozaki M, Ogawa T et al (2009) Preoperative MRI marking technique for the planning of breast-conserving surgery. Breast Cancer 16:223–228. doi:10.1007/s12282-008-0088-2
Zavagno G, Goldin E, Mencarelli R et al (2008) Role of resection margins in patients treated with breast conservation surgery. Cancer 112:1923–1931. doi:10.1002/cncr.23383
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The work was supported by Shanxi Science and Technology Research and Development grant 2003K10-G36.
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Jia, Z., Zhao, W., Fan, L. et al. The expression of PCNA, c-erbB-2, p53, ER and PR as well as atypical hyperplasia in tissues nearby the breast cancer. J Mol Hist 43, 115–120 (2012). https://doi.org/10.1007/s10735-011-9371-1
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DOI: https://doi.org/10.1007/s10735-011-9371-1