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Ultrastructural localization of fibrillin-1 and fibrillin-2 in oxytalan fibers in periodontal ligament of Japanese Macaca fuscata monkey

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Abstract

Although oxytalan fibers are a ubiquitous component of the periodontal ligament, their composition and function remain to be fully clarified. The purpose of this study was to investigate the localization of fibrillin-1 and fibrillin-2, large cysteine-rich glycoproteins, in monkey molar periodontal ligament by means of immunohistochemistry and electron microscopic immunogold labeling. Fibers positive for both fibrillin-1 and -2 were observed throughout the periodontal ligament, and their pattern of distribution was basically the same as that of oxytalan fibers. Double immunogold electron microscopy revealed that fibrillin-1 co-localized with fibrillin-2 on the microfibrils of the oxytalan fibers. The results suggest that oxytalan fibers in monkey periodontal ligament are made up of fibrillin-rich microfibrils. These results should contribute to a better understanding of the role of oxytalan fibers in development and tissue remodeling in periodontal ligament.

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Acknowledgments

The author would like thank the Primate Research Institute, Kyoto University, Japan for kindly providing us with the monkeys, and Associate Professor Jeremy Williams, Tokyo Dental College, for his assistance with the English of this manuscript.

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  1. Department of Ultrastructural Science, Tokyo Dental College, Masago 1-2-2, Mihama-ku, Chiba City, 261-8502, Japan

    Takashi Sawada

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  1. Takashi Sawada
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Correspondence to Takashi Sawada.

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Sawada, T. Ultrastructural localization of fibrillin-1 and fibrillin-2 in oxytalan fibers in periodontal ligament of Japanese Macaca fuscata monkey. J Mol Hist 41, 225–231 (2010). https://doi.org/10.1007/s10735-010-9282-6

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  • DOI: https://doi.org/10.1007/s10735-010-9282-6

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