Abstract
LIM mineralization protein 1 (LMP-1) is an essential positive regulator of osteoblast differentiation, maturation and bone formation. Our previous investigations on the distribution of LMP-1 in mature human teeth indicated that LMP-1 might play a role in the odontoblast differentiation and dentin matrix mineralization. The aim of the present study was to use immunohistochemistry to determine the expression of LMP-1 during tooth development in mouse molars. In embryonic and postnatal Kunming mice, LMP-1 protein was expressed during molar development, but the expression levels and patterns differed at various developmental stages. At embryonic day 13.5 (E13.5), LMP-1 was found in the enamel organ. At E14.5, LMP-1 was detected in the entire enamel organ and in the underlying mesenchyme. At E16.5, LMP-1 was observed in the inner and outer enamel epithelium and the stratum intermedium. The expression also converged at the cusps in the dental papilla. At E18.5 and postnatal day 2.5 (P2.5), LMP-1 was restricted to the stratum intermedium, in differentiating dental papilla cells at cusps, while it disappeared in terminal differentiated ameloblasts and odontoblasts. At P13.5, no positive staining was detected in the odontoblasts or in the dental pulp cells. Therefore, LMP-1 showed spatiotemporal expression patterns during molar development and might participate in molar crown morphogenesis and odontoblast differentiation at late molar development.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aida M, Irié T, Aida T, Tachikawa T (2005) Expression of protein kinases C betaI, betaII, and VEGF during the differentiation of enamel epithelium in tooth development. J Dent Res 84:234–239
Boden SD, Liu Y, Hair GA, Helms JA, Hu D, Racine M, Nanes MS, Titus L (1998) LMP-1, a LIM-domain protein, mediates BMP-6 effects on bone formation. Endocrinology 139:5125–5134
Bünger MH, Langdahl BL, Andersen T, Husted L, Lind M, Eriksen EF, Bünger CE (2003) Semiquantitative mRNA measurements of osteoinductive growth factors in human iliac–crest bone: expression of LMP splice variants in human bone. Calcif Tissue Int 73:446–454
Chen S, Chen L, Jahangiri A, Chen B, Wu Y, Chuang HH, Qin C, MacDougall M (2008) Expression and processing of small integrin-binding ligand N-linked glycoproteins in mouse odontoblastic cells. Arch Oral Biol 53:879–889
Feng JQ, Zhang J, Tan X, Lu Y, Guo D, Harris SE (2002) Identification of cis-DNA regions controlling Bmp4 expression during tooth morphogenesis in vivo. J Dent Res 81:6–10
Goldberg M, Septier D, Oldberg A, Young MF, Ameye LG (2006) Fibromodulin-deficient mice display impaired collagen fibrillogenesis in predentin as well as altered dentin mineralization and enamel formation. J Histochem Cytochem 54:525–537
Jernvall J, Thesleff I (2000) Reiterative signaling and patterning during mammalian tooth morphogenesis. Mech Dev 92:19–29
Kim HS, Viggeswarapu M, Boden SD, Liu Y, Hair GA, Louis-Ugbo J, Murakami H, Minamide A, Suh DY, Titus LT (2003) Overcoming the immune response to permit ex vivo gene therapy for spine fusion with human type 5 adenoviral delivery of the LIM mineralization protein–1 cDNA. Spine 28:219–226
Minamide A, Boden SD, Viggeswarapu M, Hair GA, Oliver C, Titus L (2003) Mechanism of bone formation with gene transfer of the cDNA encoding for the intracellular protein LMP–1. J Bone Joint Surg Am 85:1030–1039
Obara N (2002) Expression of the neural cell adhesion molecule during mouse tooth development. Connect Tissue Res 43:212–215
Obara N, Suzuki Y, Nagai Y, Nishiyama H, Mizoguchi I, Takeda M (2002) Expression of neural cell-adhesion molecule mRNA during mouse molar tooth development. Arch Oral Biol 47:805–813
Palacios J, Benito N, Berraquero R, Pizarro A, Cano A, Gamallo C (1995) Differential spatiotemporal expression of E- and P-cadherin during mouse tooth development. Int J Dev Biol 39:663–666
Thesleff I (2003) Epithelial-mesenchymal signaling regulating tooth morphogenesis. J Cell Sic 116:1647–1648
Thesleff I, Aberg T (1999) Molecular regulation of tooth development. Bone 25:123–125
Thesleff I, Sharpe P (1997) Signalling networks regulating dental development. Mech Dev 67:111–123
Thesleff I, Vaahtokari A, Partanen AM (1995) Regulation of organogenesis. Common molecular mechanisms regulating the development of teeth and other organs. Int J Dev Biol 39:35–50
Thesleff I, Keränen S, Jernvall J (2001) Enamel knots as signaling centers linking tooth morphogenesis and odontoblast differentiation. Adv Dent Res 15:14–18
Vaahtokari A, Aberg T, Jernvall J, Keränen S, Thesleff I (1996) The enamel knot as a signaling center in the developing mouse tooth. Mech Dev 54:39–43
Viggeswarapu M, Boden SD, Liu Y, Hair GA, Louis-Ugbo J, Murakami H, Kim HS, Mayr MT, Hutton WC, Titus L (2001) Adenoviral delivery of LIM mineralization protein–1 induces new–bone formation in vitro and in vivo. J Bone Joint Surg Am 83:364–376
Wang XY, Zhang Q, Chen Z, Zhang L (2008) Immunohistochemistry localization of LIM mineralization protein 1 in pulp-dentin complex of human teeth with normal and pathological conditions. J Endod 34:143–147
Yoon ST, Boden SD (2002) Osteoinductive molecules in orthopaedics: basic science and preclinical studies. Clin Orthop 395:33–43
Yoon ST, Park JS, Kim KS, Li J, Attallah-Wasif ES, Hutton WC, Boden SD (2004) ISSLS prize winner: LMP–1 upregulates intervertebral disc Cell production of proteoglycans and BMPs in vitro and in vivo. Spine 29:2603–2611
Zhang YD, Chen Z, Song YQ, Liu C, Chen YP (2005) Making a tooth: growth factors, transcription factors, and stem cells. Cell Res 15:301–316
Zhang Q, Wang XY, Chen Z, Liu G, Chen Z (2007) Semi-quantitative RT-PCR analysis of LIM mineralization protein 1 and its associate molecules in cultured human dental pulp cells. Arch Oral Biol 52:720–726
Acknowledgment
This work was supported by the National Natural Science Foundation of China (grant No. 30400499), Hubei Outstanding Young Investigator Project (grant No.2004ABB017) and Hubei Innovative Research Team (Grant No. 2004ABC004).
Author information
Authors and Affiliations
Corresponding author
Additional information
Pingjuan Fang and Xiaoying Wang are the co-first authors and equal contribution to this study.
Rights and permissions
About this article
Cite this article
Fang, P., Wang, X., Zhang, L. et al. Immunohistochemical localization of LIM mineralization protein 1 during mouse molar development. J Mol Hist 41, 199–203 (2010). https://doi.org/10.1007/s10735-010-9279-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10735-010-9279-1