Abstract
Previous findings have shown that P2X-purinoceptor-mediated signaling pathways regulate the release of ACh in the retina. We previously reported the existence of immunoreactivity for P2X1-, P2X2-, P2X4-, and P2X7-purinoceptors in mouse retina and speculated that P2X2 and P2X7-purinoceptors may modulate the activity of cholinergic amacrine cells. In the present study, we used an immunohistochemical technique to examine whether P2X3-, P2X5, and P2X6-purinoceptors are also important for the modulation of cholinergic amacrine cells in mouse retina. Immunoreactivity for P2X3-, P2X5-, and P2X6-purinoceptors was observed in mouse retina. Immunoreactivity for P2X3- purinoceptors was observed in the dendrites of cholinergic amacrine cells. Immunoreactivity for P2X5-purinoceptors existed in the soma of cholinergic amacrine cells. P2X6-purinoceptor immunoreactivity was not colocalized with the cholinergic amacrine cells. We concluded that, among the three P2X-purinoceptors that were examined, P2X3-purinoceptors seem to affect the function of cholinergic amacrine cells in the mouse retina.
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Acknowledgements
We thank Prof. S. Nakanishi for providing us with the transgenic mouse line. This work was supported by a Grant-in-Aid for Scientific Research (C) from JSPS (No. 18500312) to M. Kaneda.
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Shigematsu, Y., Shimoda, Y. & Kaneda, M. Distribution of immunoreactivity for P2X3, P2X5, and P2X6-purinoceptors in mouse retina. J Mol Hist 38, 369–371 (2007). https://doi.org/10.1007/s10735-007-9107-4
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DOI: https://doi.org/10.1007/s10735-007-9107-4