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Localization of precursor proteins and mRNA of type I and III collagens in usual interstitial pneumonia and sarcoidosis

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Summary

The aim of this study was to assess and compare the accumulation and distribution of newly synthesized type I and III collagens in usual interstitial pneumonia (UIP) and pulmonary sarcoidosis. Lung biopsies from 10 patients with UIP and 13 patients with sarcoidosis were investigated by immunohistochemical technique and mRNA in situ hybridization. The antibodies for the aminoterminal propeptide of type I procollagen and the aminoterminal propeptide of type III procollagen (PINP and PIIINP, respectively) were used. When compared to healthy lung, levels of type I pN- and type III pN-collagens were increased in both of these disorders. Type I procollagen was mostly present as intracellular spots in newly formed fibrosis in UIP while type III pN-collagen was expressed extracellularly underneath metaplastic alveolar epithelium. Type I procollagen was present intracellularly within and around the granulomas of sarcoidosis, whereas type III pN-collagen was expressed extracellularly, mainly around the granulomas. mRNAs of both collagens colocalized with the precursor proteins. We conclude that the expression of precursor proteins and mRNA of type I and type III collagens is increased in UIP and sarcoidosis, reflecting mainly active synthesis of these collagens in different areas of the lung.

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Acknowledgements

The technical assistance of Mr. Manu Tuovinen, Mr. Hannu Wäänänen and Mrs Liisa Kaarela is kindly acknowledged. Dr. Leila Risteli and Dr. Jukka Melkko are acknowledged for their expertise in the preparation of the purified PINP and PIIINP antibodies. Supported by the Finnish Anti-Tuberculosis Association Foundation, Juselius Foundation, the Paulo Foundation and Suomen Astra Oy.

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Authors and Affiliations

  1. Department of Pathology, University of Oulu, and Oulu University Hospital, Oulu, Finland

    Riitta Kaarteenaho-Wiik & Paavo Pääkkö

  2. Department of Internal Medicine, University of Oulu, and Oulu University Hospital, 5000, FIN-90014, Oulu, Finland

    Riitta Kaarteenaho-Wiik, Essi Lakari & Lasse Ryhänen

  3. Department of Clinical Chemistry, University of Oulu, and Oulu University Hospital, Oulu, Finland

    Juha Risteli

  4. Department of Pulmonary Medicine, Jyväskylä Central Hospital, Jyväskylä, Finland

    Lauri Lammi

  5. Department of Medicine, Pulmonary Division, University of Helsinki, Helsinki, Finland

    Vuokko L. Kinnula

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  1. Riitta Kaarteenaho-Wiik
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  2. Lauri Lammi
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Correspondence to Riitta Kaarteenaho-Wiik.

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Kaarteenaho-Wiik, R., Lammi, L., Lakari, E. et al. Localization of precursor proteins and mRNA of type I and III collagens in usual interstitial pneumonia and sarcoidosis. J Mol Hist 36, 437–446 (2005). https://doi.org/10.1007/s10735-006-9018-9

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  • DOI: https://doi.org/10.1007/s10735-006-9018-9

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