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Global profiling of alternative splicing landscape responsive to salt stress in wheat (Triticum aestivum L.)

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Abstract

Alternative splicing (AS) plays crucial roles during plant development especially in stress responses. Although many studies have been performed on AS, the function of which participating in abiotic stress responses is still not well known. To assess the interplay between AS and salt stress, genome-wide analyses of AS were performed. Totally, 11,141 genes were found exhibiting significant AS changes after salt stress treatment, and more AS events were identified in Chinese Spring (CS, salt-sensitive) (21,203) than in Qing Mai 6 (QM, salt-tolerant) (19,742). Meanwhile, wheat homeologous genes displayed differential AS responses under salt stress treatment, and 7235, 8143 and 7407 AS events were identified on subgenome A, B and D respectively. Comparison of AS events at different time after salt stress showed that 4747 and 4129 salt stress responsive AS events were characterized in CS and QM, respectively, and most AS events were induced at 48 h after salt stress (HAS) compared with the other three stages. Functional enrichment analysis found that abiotic stress responsive pathways tended to be enriched in partitioned differentially spliced genes (DSGs) between CS and QM after salt stress treatment, and Pfam classification showed that many stress related proteins were also enriched under salt stress condition. Thus, these results demonstrated that AS regulation may play key roles in response to salt stress, which, together with transcriptional regulation, contributes to abiotic stress tolerance in wheat.

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Acknowledgements

We thank Dr. Mingming Xin (College of Agronomy, China Agricultural University) for critical reading of this manuscript. This work was supported by Science and Technology Program of Colleges and Universities in Shandong Province (J17KA143) and Foundation for High-level Talents of Qingdao Agriculture University (6631118022).

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  1. Weiwei Guo and Kuohai Yu have contributed equally to this work.

Authors and Affiliations

  1. Shandong Provincial Key Laboratory of Dryland Farming Technology, Qingdao Agricultural University, Qingdao, Shandong, 266109, China

    Weiwei Guo, Liping Han, Ximei Li, Huifang Wang, Yiguo Liu & Yumei Zhang

  2. College of Agronomy, Qingdao Agricultural University, Qingdao, Shandong, 266109, China

    Weiwei Guo, Liping Han, Ximei Li, Huifang Wang, Yiguo Liu & Yumei Zhang

  3. State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, China

    Kuohai Yu

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  1. Weiwei Guo
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Contributions

All authors contributed to the study conception and design. Material preparation was collection by Liping Han, Ximei Li, Huifang Wang and Yiguo Liu. Data collection and analysis were performed by Weiwei Guo and Kuohai Yu. The first draft of the manuscript was written by Weiwei Guo and Yumei Zhang. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Yumei Zhang.

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Guo, W., Yu, K., Han, L. et al. Global profiling of alternative splicing landscape responsive to salt stress in wheat (Triticum aestivum L.). Plant Growth Regul 92, 107–116 (2020). https://doi.org/10.1007/s10725-020-00623-2

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