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Temperature influences waterlogging stress-induced damage in Arabidopsis through the regulation of photosynthesis and hypoxia-related genes

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Abstract

Waterlogging hampers plants growth and development, and its detrimental effects are strongly influenced by environmental factors. One of these factors is an ambient temperature. In this work, we showed that damage caused by waterlogging stress to Arabidopsis thaliana was less severe at lower temperatures than that at higher temperatures. The leaf photochemistry characteristics (chlorophyll fluorescence Fv/Fm, YII, ETR, and qP characteristics), chlorophyll content, and leaf temperature were more stable, and plants accumulated less malondialdehyde during waterlogging stress at low temperature (16 °C) than at elevated temperature (22 °C and/or 28 °C). Transcripts of hypoxia-related genes (such as ADH1, SUS1, PDC1, RAP2.3 and HRE1/2) were less induced after waterlogging treatment under higher temperature compared to lower temperature at early time points (3 h or 6 h) while they showed a conversed trend at later time points. Thus, we conclude that temperature may affect Arabidopsis waterlogging tolerance through the regulation of expression of hypoxia marker genes, photosynthesis, leaf transpirational cooling, and MDA accumulation.

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Abbreviations

MDA :

Malondialdehyde

TBA :

Thiobarbituric acid

Chl a :

Chlorophyll a

Chl b :

Chlorophyll b

NPQ :

Non-photochemical quenching

qP :

Photochemical quenching coefficient

ETR :

Electron transport rate

ADH1 :

Alcohol dehydrogenase 1

PDC1 :

Pyruvate dehydrogenase complex

SUS1 :

Sucrose synthase

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Acknowledgements

Financial support was provided by Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education of China (KF201605), and the open fund of Hubei Collaborative Innovation Centre for Grain Industry (LXT-16-10).

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Author notes

  1. Le Xu and Rui Pan have contributed equally to this work.

Authors and Affiliations

  1. Hubei Collaborative Innovation Centre for Grain Industry/Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, 434025, China

    Le Xu, Rui Pan & Wen-Ying Zhang

  2. School of Land and Food, University of Tasmania, Private Bag 54, Hobart, TAS, 7001, Australia

    Lana Shabala & Sergey Shabala

  3. Department of Horticulture, Foshan University, Foshan, 528000, China

    Sergey Shabala

  4. Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, 510631, China

    Le Xu

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Contributions

LX and WZ conceived and designed research. RP and LX conducted experiments and analysed data. LX and WZ wrote the manuscript. SS and LS revised the manuscript. All authors read and approved the manuscript.

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Correspondence to Sergey Shabala or Wen-Ying Zhang.

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10725_2019_518_MOESM1_ESM.tif

Fig. S1. Phenotypes of Arabidopsis plants grown under different temperatures for 36 days as control groups (TIFF 528 kb)

10725_2019_518_MOESM2_ESM.tif

Fig. S2. Expression of anaerobic genes during cold stress Microarray data showing the expression of hypoxia-related genes in Arabidopsis shoots and roots after 0 h, 0.5 h, 1 h, 3 h, 6 h, 12 h, and 24 h of cold treatment at 4 °C. Data were retrieved from the microarray database e-FP browser (http://bbc.botany.utoronto.ca/efp/cgi-bin/efpWeb.cgi, Kilian et al. 2007) (TIFF 905 kb)

10725_2019_518_MOESM3_ESM.tif

Fig. S3. Expression of anaerobic genes during heat stress. Microarray data showing the patterns of expression of hypoxia-related genes during heat stress (3 h at 38 °C followed by a recovery at 25 °C for 0 h, 0.25 h, 0.5 h, 1 h, 3 h, 4 h, 6 h, 12 h, and 24 h post-recovery). Data were obtained using the Arabidopsis eFP browserb (http://bbc.botany.utoronto.ca/efp/cgi-bin/efpWeb.cgi, Kilian et al. 2007) (TIFF 38366 kb)

10725_2019_518_MOESM4_ESM.doc

Table S2. Solubility of oxygen at different temperatures The table was downloaded from the free on-line Aquaculture Dictionary (http://www.aquatext.com/tables/oxygen.htm) (DOC 39 kb)

Table S1. A list of primers used in qRT-PCR (DOCX 12 kb)

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Xu, L., Pan, R., Shabala, L. et al. Temperature influences waterlogging stress-induced damage in Arabidopsis through the regulation of photosynthesis and hypoxia-related genes. Plant Growth Regul 89, 143–152 (2019). https://doi.org/10.1007/s10725-019-00518-x

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