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Identification of quantitative trait loci associated with tolerance to low potassium and related ions concentrations at seedling stage in rice (Oryza sativa L.)

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Abstract

Potassium (K) is one of the essential macronutrients of rice growth and eventually affects yields. To investigate the genetics of low K tolerance and related ions concentrations at the seedling stage in rice, quantitative trait loci (QTLs) were detected using a doubled haploid population derived from a cross between a japonica cultivar CJ06 and an indica accession TN1. A total of 96 QTLs were identified with phenotypic variation 5–29 %, including 30 QTLs found to be associated with shoot height, root length, shoot dry weight, root dry weight and total dry weight under the control (40 mg L−1 K+) and low K stress conditions (4 mg L−1 K+), 14 putative QTLs associated with the K tolerance coefficient of all examined traits and synthetic appraisal index, and 52 QTLs controlling four ions (Na+, K+, Ca2+ and Mg2+) concentrations of root and shoot under two treatments. The results indicated that low K tolerance and related ions concentrations were quantitatively inherited, and the detected major QTLs may be useful for marker-assistant selection.

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Abbreviations

DH:

Doubled haploid

QTLs:

Quantitative trait loci

K:

Potassium

RDW:

Root dry weight

SDW:

Shoot dry weight

TDW:

Total dry weight

SH:

Shoot height

RL:

Root length

RNC:

Root Na+ concentration

RCC:

Root Ca2+ concentration

RKC:

Root K+ concentration

RMC:

Root Mg2+ concentration

SNC:

Shoot Na+ concentration

SCC:

Shoot Ca2+ concentration

SKC:

Shoot K+ concentration

SMC:

Shoot Mg2+ concentration

KTC:

K tolerance coefficient

SAI:

Synthetic appraisal index

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Acknowledgments

This work was supported by Zhejiang Provincial Science and Technology Bureau (2012C22039); National Natural Science Foundation of China (31171535; 31101135); Hangzhou Scientific and Technological Program (20130432B04). The authors are grateful to the editors and the anonymous reviewers for their valuable comments and help.

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    Authors and Affiliations

    1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China

      Yunxia Fang

    2. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China

      Yunxia Fang, Xiaoqin Zhang, Wenyi Lu, Jiangjie Pan & Dawei Xue

    3. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China

      Weiming Wu, Jiang Hu, Longbiao Guo, Dali Zeng & Dawei Xue

    4. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Science, Hangzhou, 310021, China

      Hua Jiang

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    1. Yunxia Fang
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    Correspondence to Dali Zeng or Dawei Xue.

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    Yunxia Fang, Weiming Wu and Xiaoqin Zhang have contributed equally to this work.

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    Fang, Y., Wu, W., Zhang, X. et al. Identification of quantitative trait loci associated with tolerance to low potassium and related ions concentrations at seedling stage in rice (Oryza sativa L.). Plant Growth Regul 77, 157–166 (2015). https://doi.org/10.1007/s10725-015-0047-9

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    • DOI: https://doi.org/10.1007/s10725-015-0047-9

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