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Triploid plant regeneration from mature endosperms of Sapium sebiferum

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Abstract

Sapium sebiferum is a potential bioenergy plant that can be cultivated under various soil, water and climate conditions for both oil-rich seeds and woody biomass. An efficient protocol for regenerating triploid plants of S. sebiferum was established using mature endosperms as explants. Green and compact calli were induced from endosperms within 30 days on Murashige and Skoog medium (MS) containing 1.0–5.0 mg/l 6-benzylaminopurine (BAP) or in combination with 0.2 mg/l α-naphthalene acetic acid (NAA). Within 45 days after endosperm-derived calli were cut into pieces and cultured on media supplemented with 1.0–2.0 mg/l BAP alone or plus 0.1 mg/l NAA, more than 60 % of the callus explants initiated adventitious buds. The buds elongated into shoots after transfer onto a MS medium containing 0.1 mg/l BAP and 1.0 g/l activated charcoal. Approximate 80 % of shoots rooted on a MS medium amended with 1.0 g/l activated charcoal and 1.0 or 2.0 mg/l indole-3-butyric acid within 30 days. The triploidy of the endosperm-derived plantlets was confirmed by flow cytometric analysis, and the triploid plants grew normally after transplantation.

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Fig. 1
Fig. 2

Abbreviations

BAP:

6-benzylaminopurine

IBA:

Indole-3-butyric acid

MS:

Murashige and Skoog (1962)

NAA:

α-naphthalene acetic acid

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Acknowledgments

This work was supported by grants (No. 2006CA004 and No. 2009CDA114) from Department of Science and Technology of Hubei Province, China. We acknowledge Dr. Wenwu Guo and Miss Huiqin Wang of Huazhong Agricultural University, China for help in flow cytometry analysis. We also acknowledge Professor Yi Li of University of Connecticut, USA for help in manuscript writing.

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Authors and Affiliations

  1. Department of Biotechnology, College of Life Science, Hubei University, Wuhan, 430062, Hubei Province, China

    Liangtao Tian, Yanni Ke, Shirui Gan, Yongqin Chen, Yong Chen & Zhifan Yang

  2. Forestry Academy of Hubei Province, Wuhan, 430079, Hubei Province, China

    Xiaoguang Wang

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  1. Liangtao Tian
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  2. Yanni Ke
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  3. Shirui Gan
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  4. Yongqin Chen
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  5. Yong Chen
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  6. Zhifan Yang
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  7. Xiaoguang Wang
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Correspondence to Yongqin Chen.

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Tian, L., Ke, Y., Gan, S. et al. Triploid plant regeneration from mature endosperms of Sapium sebiferum . Plant Growth Regul 68, 319–324 (2012). https://doi.org/10.1007/s10725-012-9720-4

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