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Isolation of ambiguine D isonitrile from Hapalosiphon sp. and characterization of its phytotoxic activity

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Abstract

Cyanobacterium Hapalosiphon sp. contains phytotoxic substances. Fractionation of Hapalosiphon sp. crude extract guided by lettuce seedlings bioassay revealed that multiple fractions contained differential plant growth suppression activity. From the most active fraction inhibiting the growth of lettuce, ambiguine D isonitrile was isolated. A physiological study with ambiguine D isonitrile showed that it effectively inhibited mitosis, which resulted in the suppression of root growth. Ambiguine D isonitrile caused increased reactive oxygen species (ROS) generation and was associated with observed lipid peroxidation and cell death. These oxidative processes probably play a key role in the phytotoxic action of ambiguine D isonitrile in plants.

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Authors and Affiliations

  1. Graduate School of Life and Environmental Sciences, University of Tsukaba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan

    Intira Koodkaew, Yukari Sunohara, Shigeru Matsuyama & Hiroshi Matsumoto

Authors
  1. Intira Koodkaew
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  2. Yukari Sunohara
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  3. Shigeru Matsuyama
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  4. Hiroshi Matsumoto
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Corresponding author

Correspondence to Hiroshi Matsumoto.

Electronic supplementary material

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10725_2012_9700_MOESM1_ESM.docx

Supplementary material: 1H and 13C NMR data of ambiguine D isonitrile and summary of the characterization of the ambiguine D isonitrile. (DOCX 21 kb)

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Koodkaew, I., Sunohara, Y., Matsuyama, S. et al. Isolation of ambiguine D isonitrile from Hapalosiphon sp. and characterization of its phytotoxic activity. Plant Growth Regul 68, 141–150 (2012). https://doi.org/10.1007/s10725-012-9700-8

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  • DOI: https://doi.org/10.1007/s10725-012-9700-8

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