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Exogenous proline induces soluble sugar accumulation and alleviates drought stress effects on photosystem II functioning of Arabidopsis thaliana leaves

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Abstract

The effects of exogenous applied proline (Pro), on photosystem II (PSII) photochemistry of drought stressed (DS) 4-week old Arabidopsis thaliana plants, was studied by using chlorophyll (chl) fluorescence imaging. The maximum quantum yield of PSII photochemistry (F v /F m) in DS plants decreased significantly to 77% of that of the control value, suggesting that DS plants could not maintain PSII function, possibly due to accelerated photoinhibition of PSII. Free Pro and total soluble sugars (SS) increased, in response to DS. Exogenous foliar application of Pro by spraying, led to a remarkable increase in the accumulation of Pro and surprisingly also of SS. Both of them served to scavenge reactive oxygen species (ROS), as it was evident by the decreased lipid peroxidation level measured as malondialdehyde (MDA). DS plants sprayed with Pro showed a tolerance to photoinhibition, this indicated by F v/F m being close to values typical of healthy leaves by maintaining more than 98% of PSII function. Also the higher quantum efficiency of PSII photochemistry (Φ PSΙΙ ) and the decreased excitation pressure (1 − q p ) recorded for stressed leaves with Pro, lead us to conclude that Pro appears to be involved in the protection of chloroplast structures by quenching ROS. The enhanced dissipation of excess light energy of PSII, in part accounts for the observed increased resistance to DS in A. thaliana leaves with Pro. Our data pointed out that Pro signalling interacts with SS signaling pathway and provided a new insight in Pro metabolism.

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Acknowledgments

This research was funded by the European Fund of Regional Growth and the Hellenic General Secretariat for Research and Technology under the project No 09FR47 to M. Moustakas.

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Authors and Affiliations

  1. Department of Botany, School of Biology, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece

    Michael Moustakas, Ilektra Sperdouli & Theodora Kouna

  2. Laboratory of Pomology, School of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece

    Chrysovalantou-Irene Antonopoulou & Ioannis Therios

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  1. Michael Moustakas
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Moustakas, M., Sperdouli, I., Kouna, T. et al. Exogenous proline induces soluble sugar accumulation and alleviates drought stress effects on photosystem II functioning of Arabidopsis thaliana leaves. Plant Growth Regul 65, 315–325 (2011). https://doi.org/10.1007/s10725-011-9604-z

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