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Characterization of oxidative and antioxidative events during dehydration and rehydration of resurrection plant Ramonda nathaliae

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Abstract

In order to investigate changes of oxidative status in relation to the activity of the various protective mechanisms in resurrection plant Ramonda nathaliae, we have analysed time and relative water content (RWC) related changes in lipid peroxidation and ion leakage, hydrogen peroxide accumulation, changes of pigment content and antioxidative enzyme activity, together with expression of dehydrins. The results indicate that enhanced oxidative status during dehydration, not previously reported for resurrection plants, could play an active role in inducing the desiccation adaptive response in R. nathaliae. A critical phase is shown to exist during dehydration (in the range of RWC between 50 and 70%) during which a significant increase in hydrogen peroxide accumulation, lipid peroxidation and ion leakage, accompanied by a general decline in antioxidative enzyme activity, takes place. This phase is designated as a transition characterized by change in the type of stress response. The initial response, relying mainly on the enzymatic antioxidative system, is suspended but more effective, desiccation specific protective mechanisms, such as expression of dehydrins, are then switched on. The expression of dehydrins in R. nathaliae could be inducible as well as constitutive. In order to cope with the oxidative stress associated with rapid rewatering, R. nathaliae reactivated antioxidative enzymes. We propose that controlled elevation of reactive oxygen species, such as hydrogen peroxide, could be an important mechanism enabling resurrection plants to sense dehydration and to trigger an adaptive programme at an appropriate stage during the dehydration/rehydration cycle.

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Acknowledgment

This work was equally supported by research projects 173005 (Ž.J. and S.R.) and 173030 (T.R. and B.S.), financed by the Ministry of Science and Technological Development of Republic of Serbia. We are extremely grateful to Prof. Dr Marjetka Kidrič and dr Roger Pain, Department of Biochemistry and Molecular Biology, Jožef Štefan Institute, Ljubljana, Slovenija, for critically reading the manuscript, helpful suggestions and language editing of the manuscript, as well as to two anonymous reviewers for their valuable remarks. The authors of this paper wish to thank Dr Timothy Close, Department of Botany and Plant Science, University of California, Riverside, USA, for kindly providing the dehydrin antibodies used in this study.

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Authors and Affiliations

  1. Plant Molecular Biology Lab, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, P.O. Box 23, 11 010, Belgrade, Serbia

    Živko Jovanović

  2. Faculty of Biology, Department of Plant Ecology and Phytogeography, Institute of Botany and Botanical Garden, University of Belgrade, Takovska 43, 11 000, Belgrade, Serbia

    Tamara Rakić & Branka Stevanović

  3. Faculty of Biology, Department of Biochemistry and Molecular Biology, Institute of Physiology and Biochemistry, University of Belgrade, Studentski trg 3/II, 11 000, Belgrade, Serbia

    Svetlana Radović

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  1. Živko Jovanović
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  2. Tamara Rakić
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  3. Branka Stevanović
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  4. Svetlana Radović
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Correspondence to Živko Jovanović.

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Živko Jovanović and Tamara Rakić authors equally contributed to this work.

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Jovanović, Ž., Rakić, T., Stevanović, B. et al. Characterization of oxidative and antioxidative events during dehydration and rehydration of resurrection plant Ramonda nathaliae . Plant Growth Regul 64, 231–240 (2011). https://doi.org/10.1007/s10725-011-9563-4

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