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Regulation of exogenous spermidine on the reactive oxygen species level and polyamine metabolism in Alternanthera philoxeroides (Mart.) Griseb under copper stress

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Abstract

Effects of exogenous spermidine (Spd) on the reactive oxygen species level and polyamine metabolism against copper (Cu) stress in Alternanthera philoxeroides (Mart.) Griseb leaves were investigated. Cu treatment induced a marked accumulation of Cu and enhanced contents of malondialdehyde (MDA), hydrogen peroxide (H2O2) and the generation rate of O2 ·−. It also significantly increased putrescine (Put) levels but lowered spermidine (Spd) and spermine (Spm) levels. The activities of arginine decarboxylase (ADC), ornithine decarboxylase (ODC) and polyamine oxidase (PAO) were all elevated with the increase of Cu concentration. However, application of exogenous Spd effectively decreased H2O2 content and the generation rate of O2 ·−, prevented Cu-induced lipid peroxidation and reduced Cu accumulation. Moreover, it declined level of endogenous Put and increased levels of Spd and Spm. Activities of ADC, ODC and PAO were all inhibited by exogenous Spd. The results indicated that application of exogenous Spd could enhance the tolerance of A. philoxeroides to Cu stress by reducing the reactive oxygen level and balancing polyamine metabolism.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (No. 30670121 and No. 30870139).

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  1. Jiangsu Key Lab of Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, 1 Wenyuan Road, 210046, Nanjing, Jiangsu, People’s Republic of China

    Xiaoying Xu, Guoxin Shi, Chunxia Ding, Ye Xu, Juan Zhao, Haiyan Yang & Qiuhong Pan

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  1. Xiaoying Xu
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Correspondence to Guoxin Shi.

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Xu, X., Shi, G., Ding, C. et al. Regulation of exogenous spermidine on the reactive oxygen species level and polyamine metabolism in Alternanthera philoxeroides (Mart.) Griseb under copper stress. Plant Growth Regul 63, 251–258 (2011). https://doi.org/10.1007/s10725-010-9522-5

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