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Cadmium-induced early changes in O2 •−, H2O2 and antioxidative enzymes in soybean (Glycine max L.) leaves

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Abstract

Cadmium-induced initial changes in the production of reactive oxygen species (ROS) and antioxidant mechanism were investigated in soybean (Glycine max L. cv. Don Mario 4800 RR) leaves. Whole plants (WP) and plants without roots (PWR) were exposed to 0.0, 10.0 and 40.0 μM Cd for 0, 4, 6 and 24 h. Compared to PWR, a higher level of endogenous Cd in WP was associated with a lower oxidative stress measured in terms of lipid peroxidation. Furthermore, O2 •− content decreased in the leaves of Cd-treated WP, whereas it increased in those of Cd-treated PWR. Although O2 •− accumulation in PWR was associated with a decrease in superoxide dismutase (SOD) activity, O2 •− diminution in WP leaves was not related to any increase in SOD activity. H2O2 content increased in the leaves of both Cd-treated WP and PWR, and it was concomitant with a corresponding decline in catalase (CAT) and ascorbate peroxidase (APX) activities. When diphenyl iodonium (DPI), an inhibitor of NADPH oxidase, was added, H2O2 content remained unchanged in Cd-treated WP, suggesting that NADPH oxidase does not participate in the early hours of Cd toxicity. Taken together, our results showed that early ROS evolution and oxidative damage were different in WP and PWR. This suggests that the response in soybean leaves during the early hours of Cd toxicity is probably modulated by the root.

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Abbreviations

APX:

Ascorbate peroxidase

CAT:

Catalase

DM:

Dry mass

DPI:

Diphenyl iodonium

FM:

Fresh mass

LA:

Leaf area

MDA:

Malondialdehyde

NBT:

Nitroblue tetrazolium

PVPP:

Polyvinylpolypyrrolidone

PWR:

Plants without roots

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

WP:

Whole plants

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Acknowledgments

We thank Dr. Gabriela Pastori for her critical reading of this manuscript. We are also grateful to Dr. Adolfo Acosta and Lic. Raúl Andrés Gil (Facultad de Química, Bioquímica y Farmacia, Universidad de San Luis, San Luis, Argentina) for their technical assistance with Cd quantifications in plant tissues. This work was supported by Fundación Antorchas, Project 2004-14116-216.

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Authors and Affiliations

  1. Instituto de Fitopatología y Fisiología Vegetal (IFFIVE-INTA), Camino 60 cuadras Km 5½, CP: 5119, Cordoba, Argentina

    N. Muñoz & C. M. Luna

  2. Laboratorio de Fisiología Vegetal, Facultad de Ciencias Exactas Físicas y Naturales, Universidad Nacional de Córdoba, Velez Sarsfield 299, CP: X5000JJC, Cordoba, Argentina

    C. González

  3. Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luís, San Luis, Argentina

    A. Molina & F. Zirulnik

Authors
  1. N. Muñoz
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  2. C. González
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  3. A. Molina
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  4. F. Zirulnik
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  5. C. M. Luna
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Correspondence to C. M. Luna.

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Muñoz, N., González, C., Molina, A. et al. Cadmium-induced early changes in O2 •−, H2O2 and antioxidative enzymes in soybean (Glycine max L.) leaves. Plant Growth Regul 56, 159–166 (2008). https://doi.org/10.1007/s10725-008-9297-0

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  • DOI: https://doi.org/10.1007/s10725-008-9297-0

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