Abstract
Large amounts of sugar are imported into grape berries from source leaves during ripening, and sucrose transporters play a key role during this process. In this study, a putative grape sucrose transporter gene VvSUC27, primarily expressed in sink tissue, was transformed into a yeast strain to characterize its function as a sucrose transporter. Sucrose was taken up by yeast transformed with VvSUC27 at an optimum pH of 4.0–5.0 and a K m of 8.0–10.5 mM, indicating VvSUC27 is a LAHC (low-affinity/high-capacity) sucrose transporter. The ability of sucrose uptake in transformed yeast was activated by monosaccharides and inhibited by maltose and DEPC.
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Acknowledgements
We thank Dr. Da Peng Zhang (College of Biological Science, China Agricultural University) for providing the yeast expression vector (pDR196) and the yeast strain SUSY7/ura3. We are also grateful for his valuable comments on the sucrose uptake studies. This work was supported by China Agricultural University Research Staring Fund (grant no. 2006019) and National “948” Program (grant no. 2006-G26).
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Ya Li Zhang and Qing Yong Meng contributed equally to the paper.
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Zhang, Y.L., Meng, Q.Y., Zhu, H.L. et al. Functional characterization of a LAHC sucrose transporter isolated from grape berries in yeast. Plant Growth Regul 54, 71–79 (2008). https://doi.org/10.1007/s10725-007-9226-7
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DOI: https://doi.org/10.1007/s10725-007-9226-7