Abstract
Two litchi cultivars, a well-coloured ‘Nuomici’ and a poorly coloured ‘Feizixiao’, were used to investigate changes in endogenous abscisic acid (ABA) concentration and ethylene production during fruit maturation and to test the effects of exogenous growth regulators on litchi fruit maturation. Abscisic acid concentration in both the aril and pericarp increased with fruit maturation. Transfusion of ABA into the fruit 3 weeks before harvest accelerated, whereas transfusion of 6-benzyl aminopurine (6-BA) retarded sugar accumulation and pigmentation. The effect of 6-BA was assumed to link with the resultant decrease in ABA. In contrast, 1-aminocyclopropane-1-carboxylic acid (ACC) concentration and ACC oxidase (ACO) activities in the aril remained relatively constant during sugar accumulation. Transfusion of aminooxyacetic acid (AOA) significantly decreased ACC concentration but had no effect on sugar accumulation in the aril. These results suggested that endogenous ABA, but not ethylene, was critical for the sugar accumulation. However, the roles of ABA and ethylene in pericarp pigmentation were rather complicated. Application of exogenous ABA promoted anthocyanin synthesis significantly, but had very little effect on chlorophyll degradation. Ethylene production in litchi fruit decreased with development, but a transient increase of endogenous ethylene production was detected just around the colour break in ‘Nuomici’. Enhanced ACO activity in the pericarp was detected during pigmentation. Ethrel at 400 mg l−1 showed no effect on pericarp coloration, but accelerated chlorophyll degradation and anthocyanin synthesis at a much higher concentration (800 mg l−1). Fruit dipped in ABA solution alone yielded no effect on chlorophyll degradation, but the combined use of ABA and Ethrel at 400 mg l−1 enhanced both chlorophyll degradation and anthocyanin synthesis. These results indicated the possible synergistic action of ethylene and ABA during litchi fruit colouration. ABA is suggested to play a more crucial role in anthocyanin synthesis, while ethylene is more important in chlorophyll degradation. ABA can increase the sensitivity of pericarp tissue to ethylene.
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Abbreviations
- ABA:
-
Abscisic acid
- 6-BA:
-
6-Benzyl aminopurine
- AOA:
-
Aminooxyacetic acid
- ACC:
-
1-Aminocyclopropane-1-carboxylic acid
- ACO:
-
ACC oxidase
References
Abeles FB, Morgan PW, Saltveit Jr (1992) Ethylene in plant biology, 2nd edn. Academic, New York, p 581
Akamine EK, Goo T (1973) Respiration and ethylene production during ontogeny of fruit. J Amer Soc Hort Sci 98:381–383
Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoxidase in Beta vulgaris. Plant Physiol 24:1–5
Brady CJ (1987) Fruit ripening. Ann Rev Plant Physiol 38:155–178
Chervin C, El-Kereamy A, Roustan JP, Latche A, Lamon J, Bouzayen M (2004) Ethylene seems required for the berry development and ripening in grape, a non-climacteric fruit. Plant Sci 167:1301–1305
Coombe BG (1976) The development of fresh fruits. Ann Rev Plant Physiol 27:207–227
Coombe BG, Hale HZ (1973) The hormone content of ripening grape berries and the effects of growth substance treatments. Plant Physiol 51:629–634
Giovannoni J (2001) Molercular biology of fruit maturation and ripening. Ann Rev Plant Physiol Plant Mol Biol 52:725–749
Hamilton AJ, Lycett GW, Grierson D (1990) Antisense gene that inhibits synthesis of the hormone ethylene in transgenic plants. Nature 346:284–287
Han DH, Lee CH (2004) The effects of GA3, CPPU and ABA application on the quality of kyoho (Vitis vinifera L.x V. labrusca L.) grape. Acta Hort 640:193–197
Huang XM (1998) Studies on the mechanisms of some veraison-related physiological aspects in grape berries. Ph.D. Dissertation, South China Agricultural University, Guangzhou, China
Jiang JP, Su MX, Lee PM (1986) The production and physiological effects of ethylene during ontogeny and after harvest of litchi fruits. Acta Phytophysiol Sin 12:95–103
Katz E, Lagunes PM, Riov J, Weiss D, Goldschmidt EE (2004) Molecular and physiological evidence suggests the existence of a system II-like pathway of ethylene production in non-climacteric citrus fruit. Planta 219:243–252
Kondo S, Gemma H (1993) Relationship between abscisic acid (ABA) content and maturation of sweet cherry. J Jpn Soc Hort Sic 62:63–68
Kondo S, Inoue K (1997) Abscisic acid (ABA) and 1-aminocyclopropane-1-carboxylic acid (ACC) content during growth of ‘Satohnishiki’ cherry fruit, and the effect of ABA and ethephon application on fruit quality. J Hort Sci 72:221–227
Kondo S, Uthaibutra J, Gemma H (1991) Comparison of 1-aminocyclopropane-1-carboxylic acid, abscisic acid and anthocyanin content of some apple cultivars during fruit growth and maturation. J Jpn Soc Hort Sic 60:505–511
Lara I, Vendrell M (1998) Relationship between ethylene, abscisic acid and quality during postharvest storage of ‘Granny Smith’ apple. Postharv Biol Tech 13:11–18
Lara I, Vendrell M (2000a) Changes in abscisic acid levels, ethylene biosynthesis and protein patterns during fruit maturation of apples. J Amer Soc Hort Sci 125:183–189
Lara I, Vendrell M (2000b) Development of ethylene-synthesizing capacity in preclimacteric apples: interaction between abscisic acid and ethylene. J Amer Soc Hort Sci 125:505–512
Lizada MCC, Yang SF (1979) A simple and sensitive assay for ACC. Anal Biochem 100:140–145
Ma BZ, Cheng YE (1984) The method of testing anthocyanin content in apple pericarp. China Fruits 4:49–51
Martinez GA, Chaves AR, Anon MC (1996) Effect of exogenous application of gibberellin acid on color change and phenylalanine ammonia-lyase, chlorophyllase, and peroxidase activities during ripening of strawberry fruit (Fragaria × ananassa Duch.). J Plant Growth Regul 15:139–146
Pech PC, Sharkawi I, Chaves A, Li Z, Lelievre JM, Bouzayen M, Zegzouti H, Latche A (2002) Recent developments on the role of ethylene in the ripening of climacteric fruit. Acta Hort 587:489–495
Riov J, Dagan E, Goren R, Yang SF (1990) Characterization of abscisic acid-induced ethylene production in citrus leaf and tomato fruit tissues. Plant Physiol 92:48–53
Sharma SB, Ray PK, Rai R (1986) The use of growth regulators for early ripening of litchi (Litchi chinensis Sonn.). J Hort Sci 61:533–534
Terai H (1993) Behaviors of 1-aminocyclopropane-1-carboxylic acid (ACC) and ACC synthase responsible for ethylene production in normal and mutant (nor and rin) tomato fruits at various ripening stages. J Jpn Soc Hort Sci 61:805–812
Wang HC, Huang HB, Huang XM, Hu ZQ (2006) Sugar and acid compositions in the arils of Litchi chinensis Sonn.: cultivar differences and evidence for the absence of succinic acid. J Hort Sci Biotech 81:57–62
Wu YM, Gu CQ, Tai GF, Liu Y (1992) The role of ABA and ethylene in the ripening and senescence of strawberry fruits. Acta Phytophysiol Sin 18:167–172
Yuan RC, Huang HB (1988) Litchi fruit abscission: its pattern, effect of shading and relation to endogenous abscisic acid. Sci Hort 36:281–292
Zhang W, Lü ZS (1983) Relationship between abscisic acid and apricot fruit ripening. Acta Bot Sin 25:537–543
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This project was supported by the National Natural Science Foundation of China (Contract No. 30200188).
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Wang, H., Huang, H. & Huang, X. Differential effects of abscisic acid and ethylene on the fruit maturation of Litchi chinensis Sonn.. Plant Growth Regul 52, 189–198 (2007). https://doi.org/10.1007/s10725-007-9189-8
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DOI: https://doi.org/10.1007/s10725-007-9189-8