Skip to main content
SpringerLink
Log in

Reduction of hyperhydricity in the culture of Lepidium meyenii shoots by the addition of rare earth elements

  • Original Paper
  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

Adventitious shoots induced from maca calli on induction media without rare earth elements (REE) had higher water content and lower soluble protein concentration when compared with shoots sprouted from maca seeds. Due to lower activities of antioxidative enzymes, there were higher concentrations of H2O2 and malonyldialdehyde (MDA) in adventitious shoots than those in seed shoots. When La3+, Ce3+ and Nd3+ (0.04 mM to 0.1 mM) were added to induction media, induction rates of the adventitious shoots were only affected slightly, but hyperhydricity rates were significantly reduced. La3+, Ce3+ or Nd3+ enhanced the activities of antioxidative enzymes in adventitious shoots, e.g. peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), superoxide dismutase (SOD), monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR). When the concentrations of La3+, Ce3+ and Nd3+ were 0.1 mM, the oxygen stress in adventitious shoots was decreased to levels similar to seed shoots, where most adventitious shoots grew normally.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Abbreviations

APX:

Ascorbate peroxidase

CAT:

Catalase

DPPH:

1,1-diphenyl-2-picrylhydarazyl

GR:

Glutathione reductase

MDA:

Malonyldialdehyde,

MDHAR:

Monodehydroascorbate reductase

MS:

Murashige and Skoog’s (1962) medium

NBT:

Nitrotetrazolium blue tetrazolium

POD:

Peroxidase

REE:

Rare earth elements

SOD:

Superoxide dismutase

TBA:

Thiobarbituric acid

TCA:

Trichloroacetic acid

References

  • Azevedo Neto AD, Prisco JT, Enéas-Filho J, Abreu CEB, Gomes-Filho E (2006) Effect of salt stress on antioxidative enzymes and lipid perosidation in leaves and roots of salt-tolerant and salt-sensitive maize genotypes. Environ Exp Bot 56:87–94

    Article  Google Scholar 

  • Beyer WF, Fridovic I (1987) Assaying for superoxide dismutase activity: some large consequences of minor changes in condition. Anal Biochem 161:559–566

    Article  PubMed  CAS  Google Scholar 

  • Cassells AC, Curry RF (2001) Oxidative stress and physiological, epigenetic and genetic variability in plant tissue culture: implications for micropropagators and genetic engineers. Plant Cell Tiss Org 64:145–157

    Article  CAS  Google Scholar 

  • Chen J, Ziv M (2001) The effect of ancymidol on hyperhydricity, regeneration, starch and antioxidant enzymatic activities in liquid-cultured Narcissus. Plant Cell Rep 20:22–27

    Article  Google Scholar 

  • Cicero AF, Piacente S, Plaza A, Sala E, Arletti R, Pizza C (2002) Hexanic Maca extract improves rat sexual performance more effectively than methanolic and chloroformic Maca extracts. Andrologia 34:177–179

    Article  PubMed  CAS  Google Scholar 

  • Dily FL, Huault C, Gaspar T, Billard JP (1993) Does altered nitrogen metabolism and H2O2 accumulation explain the vitrified status of the fully habituated callus of Beta vulgaris (L.)? Plant Cell Tiss Org 35:69–74

    Article  Google Scholar 

  • Franck T, Gaspar T, Kevers C, Penel C, Dommes J, Hausman JF (2001) Are hyperhydric shoots of Prunus avium L. energy deficient? Plant Sci 160:1145–1151

    CAS  Google Scholar 

  • Franck T, Kevers C, Gaspar T, Dommes J, Deby C, Greimers R, Serteyn D, Deby-Dupont G (2004) Hyperhydricity of Prunus avium shoots cultured on gelrite: a controlled stress response. Plant Physiol Biochem 42:519–527

    Article  PubMed  CAS  Google Scholar 

  • Gonzales GF, Cordova A, Vega K, Chung A, Villena A, Gonez C, Castillo S (2002) Effect of Lepidium meyenii (MACA) on sexual desire and its absent relationship with serum testosterone levels in adult healthy men. Andrologia 34:367–372

    Article  PubMed  CAS  Google Scholar 

  • Guo CR, Shi RX, Wang JS, Cheng YX (2000) Protective effect of Cerium on Chloroplast in wheat seeding. J Chin Rare Earths Soc 18:367–370 [In China]

    CAS  Google Scholar 

  • Hossain MA, Nakano Y, Asada K (1984) Monodehydroascorbate reductase in spinach chloroplasts and its participation in regeneration of ascorbate for scavenging hydrogen peroxide. Plant Cell Physiol 25:385–395

    CAS  Google Scholar 

  • Hu QH, Ye ZJ (1996) Physiological effects of rare-earth elements on plants. Plant Physiol Commun 32:296–300 [In China]

    CAS  Google Scholar 

  • Kevers C, Franck T, Strasser RJ, Dommes J, Gaspar T (2004) Hyperhydricity of micropropagated shoots: a typically stress-induced change of physiological state. Plant Cell Tiss Org 77:181–191

    Article  Google Scholar 

  • Li HS (eds) (2000) Principles and techniques of plant physiological biochemical experiment. Higher Education Press, Beijing

    Google Scholar 

  • Lee KJ, Dabrowski K, Sandoval M, Miller MJS (2005) Activity-guided fractionation of phytochemicals of maca meal, their antioxidant activities and effects on growth, feed utilization, and survival in rainbow trout (Oncorhynchus mykiss) juveniles. Aquaculture 244:293–301

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Olmos E, Piqueras A, Martínez-Solano JR, Hellín E (1997) The subcellular localization of peroxidase and the implication of oxidative stress in hyperhydrated leaves of regenerated carnation. Plant Sci 130:97–105

    Article  CAS  Google Scholar 

  • Peng A, Pang X (2002) The free radical mechanism of rare earth elements in anti-adversity for plants. Environ Chem 21:313–317 [In China]

    CAS  Google Scholar 

  • Pütter J (1974) Peroxidase. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York, p 673–684

    Google Scholar 

  • Rea J (1992) Raíces andinas: maca. In: Bermejo H, León JE (eds) Cultivos marginados, otra perspectiva de 1492. FAO Publ, p 163–166

  • Saher S, Fernándea-García N, Piqueras A, Hellín E, Olmos E (2005) Reducing properties, energy efficiency and carbohydrate metabolism in hyperhydric and normal carnation shoots cultured in vitro: a hypoxia stress? Plant Physiol Biochem 43:573–582

    Article  PubMed  CAS  Google Scholar 

  • Türkan I, Bor M, Özdemir F, Koca H (2005) Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Sci 168:223–231

    Article  Google Scholar 

  • Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain-treated been plants protective role of exogenous polymines. Plant Sci 151:59–66

    Article  CAS  Google Scholar 

  • Wang X, Shi GX, Xu QS, Wang CT (2004) Toxic effects of lanthamum, cerium, chromium and zinc on pata mogeton malaianus. J Chin Rare Earths Soc 22:682–686 [In China]

    CAS  Google Scholar 

  • Wang JS, Guo CR, Cheng YX (1997) Mechanism of cerium ion clearing superoxide radical. J Chin Rare Earths Soc 15:151–154 [In China]

    CAS  Google Scholar 

  • Yuan XF, Wang Q, Zhao B, Wang YC (2002) Improved cell growth and total flavonoids of Saussurea medusa on solid culture medium supplemented with rare earth elements. Biotechnol Lett 24:1889–1892

    Article  CAS  Google Scholar 

  • Zheng BL, He K, Kim CH, Rogers L, Shao Y, Huang ZY, Lu Y, Yan SJ, Qien LC, Zheng QY (2000a) Effect of a lipidic extract from Lepidium meyenii on sexual behavior in mice and rats. Urology 55:598–602

    Article  PubMed  CAS  Google Scholar 

  • Zheng HL, Zhao ZQ, Zhang CG, Feng JC, Su MJ (2000b) Advances in mechanism research of rare earth biological effect. Chin Rare Earths 21:55–60 [In China]

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing, 100080, P.R. China

    Ya-Li Wang, Xiao-Dong Wang, Bing Zhao & Yu-Chun Wang

  2. Graduate School of Chinese Academy of Sciences, Beijing, 100049, P.R. China

    Ya-Li Wang

Authors
  1. Ya-Li Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiao-Dong Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bing Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yu-Chun Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yu-Chun Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, YL., Wang, XD., Zhao, B. et al. Reduction of hyperhydricity in the culture of Lepidium meyenii shoots by the addition of rare earth elements. Plant Growth Regul 52, 151–159 (2007). https://doi.org/10.1007/s10725-007-9185-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10725-007-9185-z

Keywords

Search

Navigation