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Role of Calcium on Phenolic Compounds and Enzymes Related to Lignification in Soybean (Glycine max L.) Root Growth

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Abstract

Changes in soluble and cell wall bound peroxidases activities, phenylalanine ammonia-lyase activity and phenolic compounds and lignin contents in roots of calcium-treated soybean (Glycine max (L.) Merr.) seedlings and their relationships with root growth were investigated. Three-day-old soybean seedlings were cultivated in nutrient solution with or without 0.025–5.0 mM calcium for 24 h. In general, length and fresh and dry weights of roots increased, while activities of enzymes (soluble and cell-wall peroxidases and phenylalanine ammonia-lyase) and phenolic compounds and lignin contents decreased against calcium concentrations. In the absence of calcium, phenylalanine ammonia-lyase and peroxidases activities increased by accumulating phenolic compounds and lignin due to restricted growth of roots. Enhanced calcium supply reduced the production of phenolic compounds and lignification due to low phenylalanine ammonia-lyase and peroxidases activities, reinforcing the essential role of calcium to improve the soybean root growth.

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Abbreviations

Ca2+ :

calcium

PAL:

phenylalanine ammonia-lyase

POD:

peroxidase

References

  • M.R. Broadley H.C. Bowen H.L. Cotterill J.P. Hammond M.C. Meacham A. Mead P.J. White (2003) ArticleTitleVariation in the shoot calcium content of angiosperms J. Exp. Bot. 54 1431–1446 Occurrence Handle12709490 Occurrence Handle1:CAS:528:DC%2BD3sXjt1Ogs7k%3D Occurrence Handle10.1093/jxb/erg143

    Article  PubMed  CAS  Google Scholar 

  • I. Cakmak W.J. Horst (1991) ArticleTitleEffect of aluminum on lipid peroxidation, superoxide dismutasecatalaseand peroxidase activities in root tips of soybean (Glycine max) Physiol. Plant. 83 463–468 Occurrence Handle1:CAS:528:DyaK38XjsFymuw%3D%3D Occurrence Handle10.1111/j.1399-3054.1991.tb00121.x

    Article  CAS  Google Scholar 

  • P. Castañeda L.M. Pérez (1996) ArticleTitleCalcium ions promote the response of citrus lemon against fungal elicitors or wounding Phytochemistry 42 595–598 Occurrence Handle10.1016/0031-9422(95)00981-7

    Article  Google Scholar 

  • N. Chishaki T. Horiguchi (1997) ArticleTitleResponses of secondary metabolism in plants to nutrient deficiency Soil Sci. Plant Nutr. 43 987–991 Occurrence Handle1:CAS:528:DyaK1cXhslGjtb0%3D

    CAS  Google Scholar 

  • J.H. Christensen G. Bauw K.G. Welinder M. Montagu ParticleVan W. Boerjan (1998) ArticleTitlePurification and characterization of peroxidases correlated with lignification in poplar xylem Plant Physiol. 118 125–135 Occurrence Handle9733532 Occurrence Handle1:CAS:528:DyaK1cXmtV2mtLY%3D Occurrence Handle10.1104/pp.118.1.125

    Article  PubMed  CAS  Google Scholar 

  • L.A. Donaldson (2001) ArticleTitleLignification and lignin topochemistry – an ultrastructural view Phytochemistry 57 859–876 Occurrence Handle11423137 Occurrence Handle1:CAS:528:DC%2BD3MXksVygsLg%3D Occurrence Handle10.1016/S0031-9422(01)00049-8

    Article  PubMed  CAS  Google Scholar 

  • M.L.L. Ferrarese J.D. Rodrigues O. Ferrarese-Filho (2000) ArticleTitlePhenylalanine ammonia-lyase activity in soybean roots extract measured by reversed-phase high performance liquid chromatography Plant Biol. 2 152–153 Occurrence Handle1:CAS:528:DC%2BD3cXjs1WntLk%3D Occurrence Handle10.1055/s-2000-9162

    Article  CAS  Google Scholar 

  • M.L.L. Ferrarese A. Zottis O. Ferrarese-Filho (2002) ArticleTitleProtein-free lignin quantification in soybean (Glycine max) roots Biologia 57 541–543 Occurrence Handle1:CAS:528:DC%2BD38XnvVCrtbo%3D

    CAS  Google Scholar 

  • S.C. Fry (2004) ArticleTitlePrimary cell wall metabolism: tracking the careers of wall polymers in living plant cells New Phytol. 161 641–675 Occurrence Handle1:CAS:528:DC%2BD2cXhvFehsb8%3D Occurrence Handle10.1111/j.1469-8137.2004.00980.x

    Article  CAS  Google Scholar 

  • V. Herrig M.L.L. Ferrarese L.S. Suzuki J.D. Rodrigues O. Ferrarese-Filho (2002) ArticleTitlePeroxidase and phenylalanine ammonia-lyase activities, phenolic acid contents, and allelochemicals-inhibited root growth of soybean Biol. Res. 35 59–66 Occurrence Handle12125206 Occurrence Handle1:CAS:528:DC%2BD38XntFymtL0%3D Occurrence Handle10.4067/S0716-97602002000100009

    Article  PubMed  CAS  Google Scholar 

  • T. Kawai M. Hikawa Y. Ono (1995) ArticleTitleEffects of calcium-sulfate and sublimed sulfur on incident of internal browning in roots of Japanese radish J. Jpn. Soc. Hort. Sci. 64 79–84 Occurrence Handle1:CAS:528:DyaK2MXmvFSgs7c%3D Occurrence Handle10.2503/jjshs.64.79

    Article  CAS  Google Scholar 

  • D. Ke M.E. Saltveit (1986) ArticleTitleEffects of calcium and auxin on russet spotting and phenylalanine ammonia-lyase activity in iceberg lettuce HortSci. 21 1169–1171 Occurrence Handle1:CAS:528:DyaL2sXjvF2i

    CAS  Google Scholar 

  • Y.E. Kolupaev G.E. Akinina A.V. Mokrousov (2005) ArticleTitleInduction of heat tolerance in wheat coleoptiles by calcium ions and its relation to oxidative stress Russ. J. Plant Physiol. 52 199–204 Occurrence Handle1:CAS:528:DC%2BD2MXjtFehsr0%3D Occurrence Handle10.1007/s11183-005-0030-9

    Article  CAS  Google Scholar 

  • P.M. Kopittke N.W. Menzies (2005) ArticleTitleEffect of pH on Na induced Ca deficiency Plant Soil 269 119–129 Occurrence Handle1:CAS:528:DC%2BD2MXks1Ojtbo%3D Occurrence Handle10.1007/s11104-004-0395-0

    Article  CAS  Google Scholar 

  • J.F. Loneragan K. Snowball W.J. Simmons (1968) ArticleTitleResponse of plants to calcium concentrations in solution culture Aust. J. Agric. Res. 19 845–857 Occurrence Handle1:CAS:528:DyaF1MXns1ynsg%3D%3D Occurrence Handle10.1071/AR9680845

    Article  CAS  Google Scholar 

  • Z.F. Lund (1970) ArticleTitleThe effect of calcium and its relation to several cations in soybean root growth Soil Sci. Soc. Am. Proc. 34 456–459 Occurrence Handle1:CAS:528:DyaE3cXktlCgsbo%3D Occurrence Handle10.2136/sssaj1970.03615995003400030030x

    Article  CAS  Google Scholar 

  • H. Marschner C. Richter (1974) ArticleTitleCalcium-transport in roots of maize and bean plants Plant Soil 40 193–210 Occurrence Handle1:CAS:528:DyaE2cXotFeksg%3D%3D Occurrence Handle10.1007/BF00011422

    Article  CAS  Google Scholar 

  • Marschner H. 1995. Mineral Nutrition of Higher Plants. Academic Press. 2nd ed., 889 pp.

  • M. Matsuki (1996) ArticleTitleRegulation of plant phenolic synthesis: from biochemistry to ecology and evolution Aust. J. Bot. 44 613–634 Occurrence Handle1:CAS:528:DyaK2sXnsVKhtQ%3D%3D Occurrence Handle10.1071/BT9960613

    Article  CAS  Google Scholar 

  • S.B. McLaughlin R. Wimmer (1999) ArticleTitleCalcium physiology and terrestrial ecosystem process New Phytol. 142 373–417 Occurrence Handle1:CAS:528:DyaK1MXlsFGrs7c%3D Occurrence Handle10.1046/j.1469-8137.1999.00420.x

    Article  CAS  Google Scholar 

  • F. Passardi C. Penel C. Dunand (2004) ArticleTitlePerforming the paradoxical: how plant peroxidases modify the cell wall Trends Plant Sci. 9 534–540 Occurrence Handle15501178 Occurrence Handle1:CAS:528:DC%2BD2cXovFWrtL4%3D Occurrence Handle10.1016/j.tplants.2004.09.002

    Article  PubMed  CAS  Google Scholar 

  • J.C. Pintro G.J. Taylor (2005) ArticleTitleCalcium requirement in the background nutrient solution on growth of wheat plants using the relative addition rate technique J. Plant. Nutr. 28 551–565 Occurrence Handle1:CAS:528:DC%2BD2MXjslejt7c%3D

    CAS  Google Scholar 

  • J. Ralph K. Lundquist G. Brunow F. Lu H. Kim P.F. Schatz J.M. Marita R.D. Hatfield S.A. Ralph J.H. Christensen W. Boerjan (2004) ArticleTitleLignins: natural polymers from oxidative coupling of 4-hydroxyphenyl-propanoids Phytochem. Rev. 3 29–60 Occurrence Handle1:CAS:528:DC%2BD2MXhsFehu74%3D Occurrence Handle10.1023/B:PHYT.0000047809.65444.a4

    Article  CAS  Google Scholar 

  • A.S.N. Reddy (2001) ArticleTitleCalcium: silver bullet in signaling Plant Sci. 160 381–404 Occurrence Handle11166425 Occurrence Handle1:CAS:528:DC%2BD3MXpsFahsA%3D%3D Occurrence Handle10.1016/S0168-9452(00)00386-1

    Article  PubMed  CAS  Google Scholar 

  • A. Ros Barceló L.V. Gómez Ros C. Gabaldón M. López-Serrano F. Pomar J.S. Carrión M.A. Pedreno (2004) ArticleTitleBasic peroxidases: the gateway from lignin evolution? Phytochem. Rev. 3 61–78 Occurrence Handle10.1023/B:PHYT.0000047803.49815.1a

    Article  Google Scholar 

  • J.M. Ruiz R.M. Rivero I. López-Cantarero L. Romero (2003) ArticleTitleRole of Ca2+ in the metabolism of phenolic compounds in tobacco leaves (Nicotiana tabacum L.) Plant Growth Reg. 41 173–177 Occurrence Handle1:CAS:528:DC%2BD3sXoslShs7k%3D Occurrence Handle10.1023/A:1027358423187

    Article  CAS  Google Scholar 

  • W.D. Santos M.L.L. Ferrarese A. Finger A.C.N. Teixeira O. Ferrarese-Filho (2004) ArticleTitleLignification and related enzymes in Glycine max root growth-inhibition by ferulic acid J. Chem. Ecol. 30 1199–1208

    Google Scholar 

  • F.A. Tomás-Barberán M.I. Gil M. Castañer F. Artés M.E. Saltveit (1997) ArticleTitleEffect of selected browing inhibitors on phenolic metabolism in stem tissue of harvested lettuce J. Agric. Food Chem. 45 583–589 Occurrence Handle10.1021/jf960478f

    Article  Google Scholar 

  • N.C. Vitch (2004) ArticleTitleHorseradish peroxidase: a modern view of a classic enzyme Phytochemistry 65 249–259 Occurrence Handle10.1016/j.phytochem.2003.10.022 Occurrence Handle1:CAS:528:DC%2BD2cXmvFGntA%3D%3D

    Article  CAS  Google Scholar 

  • P.J. White (1998) ArticleTitleCalcium channels in the plasma membrane of root cells Ann. Bot. 81 173–183 Occurrence Handle1:CAS:528:DyaK1cXhsVOqtrc%3D Occurrence Handle10.1006/anbo.1997.0554

    Article  CAS  Google Scholar 

  • P.J. White M.R. Broadley (2003) ArticleTitleCalcium in plants Ann. Bot. 92 487–511 Occurrence Handle12933363 Occurrence Handle1:CAS:528:DC%2BD3sXoslOmtLg%3D Occurrence Handle10.1093/aob/mcg164

    Article  PubMed  CAS  Google Scholar 

  • N. Zieslin M. Abolitz (1994) ArticleTitleLeakage of phenolic- compounds from plant-root: effects of pH, Ca2+NaCl Sci. Hort. 58 303–314 Occurrence Handle1:CAS:528:DyaK2MXitFWgsbw%3D Occurrence Handle10.1016/0304-4238(94)90100-7

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringá, Av. Colombo, 5790, 87020-900, Maringá, PR, Brazil

    Aline Finger Teixeira, Aneliz de Bastos Andrade, Osvaldo Ferrarese-Filho & Maria de Lourdes Lucio Ferrarese

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  1. Aline Finger Teixeira
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  2. Aneliz de Bastos Andrade
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  3. Osvaldo Ferrarese-Filho
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  4. Maria de Lourdes Lucio Ferrarese
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Correspondence to Maria de Lourdes Lucio Ferrarese.

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Teixeira, A.F., de Bastos Andrade, A., Ferrarese-Filho, O. et al. Role of Calcium on Phenolic Compounds and Enzymes Related to Lignification in Soybean (Glycine max L.) Root Growth. Plant Growth Regul 49, 69–76 (2006). https://doi.org/10.1007/s10725-006-0013-7

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  • DOI: https://doi.org/10.1007/s10725-006-0013-7

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