Skip to main content
SpringerLink
Log in

Effect of 5-Aminolevulinic Acid on Development and Salt Tolerance of Potato (Solanum tuberosum L.) Microtubers in vitro

  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

The effects of 5-aminolevulinic acid (ALA), a key precursor in the biosynthesis of porphyrins such as chlorophyll and heme, on development and salt tolerance of microtubers of two potato (Solanum tuberosum L.) cultivars Jingshi-2 and Zihuabai were examined under in vitro conditions. ALA at 0.3–3 mg/l promoted microtuber formation by increasing the average number, diameter, and fresh weight of microtubers especially under 0.5% NaCl stress conditions, but further increase in ALA concentration resulted in a reduction of microtuber yield irrespective of NaCl stress. Under 1.0% NaCl stress conditions, microtuberization was seriously repressed and could not be restored by the addition of ALA. The accumulation of malondialdehyde in the microtubers treated with 30 mg/l ALA increased by 22% compared to the controls (no salinity), while only a 7% increase was observed when the microtubers were exposed to 0.5% NaCl, indicating that ALA functions as a protectant against oxidative damages of membranes. Under 0.5% NaCl stress conditions, the highest activities of peroxidase and polyphenoloxidase were detected in microtubers treated with ALA at 0.3 and 3 mg/l, being by 73% and by 28% greater than those in the untreated controls, respectively. These results demonstrate that ALA at lower concentrations of 0.3–3 mg/l promotes development and growth of potato microtubers in vitro and enhances protective functions against oxidative stresses, but ALA at 30 mg/l and higher concentrations seems to induce oxidative damage probably through formation and accumulation of photooxidative porphyrins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ALA:

5-aminolevulinic acid

BAP:

benzylaminopurine

CCC:

chlorocholine chloride

MDA:

malondialdehyde

MS medium:

Murashige and Skoog medium

POD:

peroxidase

PPO:

polyphenoloxidase

ROS:

reactive oxygen species

Vc:

vitamin C

References

  • InstitutionalAuthorNameAnonymous (Shanghai Plant Physiology Institute of Chinese Academy of Sciences & Shanghai Plant Physiology Society) (1999) Experimental Guide of Modern Plant Physiology Science Press Beijing, China 127–128

    Google Scholar 

  • S.I. Beale (1990) ArticleTitleBiosynthesis of the tetrapyrrole pigment precursorδ-aminolevulinic acidfrom glutamate Plant Physiol. 93 1273–1279 Occurrence Handle16667613 Occurrence Handle1:CAS:528:DyaK3cXls1yqsb4%3D

    PubMed  CAS  Google Scholar 

  • P.A. Castelfranco P.M. Rich S.I. Beale (1974) ArticleTitleThe abolition of the lag phase in greening cucumber cotyledons by exogenous δ-aminolevulinic acid Plant Physiol. 53 615–618 Occurrence Handle16658752 Occurrence Handle1:CAS:528:DyaE2cXkt1aitbY%3D Occurrence Handle10.1104/pp.53.4.615

    Article  PubMed  CAS  Google Scholar 

  • D. Evers C. Schmit Y. Mailliet J.F. Hausman (1997) ArticleTitleGrowth characteristics and biochemical changes of poplar shoots in vitro under sodium chloride stress J. Plant Physiol. 151 748–753 Occurrence Handle1:CAS:528:DyaK1cXis1Sjtg%3D%3D

    CAS  Google Scholar 

  • FAO. 1995. Potatoes in the 1990s. Situations and Prospects of World Potato Economy. Publ No. M-71, FAO, Rome, 39pp.

  • A.R. Fernie L. Willmitzer R.N. Trethewey (2002) ArticleTitleSucrose to starch: a transition in molecular plant physiology Trends Plant Sci. 7 35–41 Occurrence Handle11804825 Occurrence Handle1:CAS:528:DC%2BD38Xntl2gsQ%3D%3D Occurrence Handle10.1016/S1360-1385(01)02183-5

    Article  PubMed  CAS  Google Scholar 

  • D.R. Gossett E.P. Millhollon M.C. Lucas (1994) ArticleTitleAntioxidant response to NaCl stress in salt-tolerant and saltsensitive cultivars of cotton Crop Sci. 34 706–714 Occurrence Handle1:CAS:528:DyaK2cXltlSiu7g%3D Occurrence Handle10.2135/cropsci1994.0011183X003400030020x

    Article  CAS  Google Scholar 

  • H. Greenway R. Munns (1980) ArticleTitleMechanisms of salt tolerance in nonhalophytes Ann. Rev. Plant Physiol. 31 149–190 Occurrence Handle1:CAS:528:DyaL3cXksVWntb4%3D Occurrence Handle10.2307/2442345

    Article  CAS  Google Scholar 

  • Y. Hotta T. Tanaka L. Bingshan Y. Takeuchi M. Konnai (1998) ArticleTitleImprovement of cold resistance in rice seedlings by 5-aminolevulinic acid J. Pesticide Sci. 23 29–33 Occurrence Handle1:CAS:528:DyaK1cXhs1eqt7Y%3D Occurrence Handle10.1086/337245

    Article  CAS  Google Scholar 

  • Y. Hotta T. Tanaka H. Takaoka Y. Takeuchi M. Konnai (1997a) ArticleTitleNew physiological effects of 5-aminolevulinic acid in plants: the increased photosynthesis, chlorophyll contentand plant growth Biosci. Biotech. Biochem. 61 2025–2028 Occurrence Handle1:CAS:528:DyaK1cXltFSnuw%3D%3D Occurrence Handle10.1271/bbb.61.2025

    Article  CAS  Google Scholar 

  • Y. Hotta T. Tanaka H. Takaoka Y. Takeuchi M. Konnai (1997b) ArticleTitlePromotive effects of 5-aminolevulinic acid on the yield of several crops Plant Growth Regul. 22 109–144 Occurrence Handle1:CAS:528:DyaK2sXmt1yitb8%3D Occurrence Handle10.1023/A:1005883930727

    Article  CAS  Google Scholar 

  • Y.Q. Ke T.G. Pan (1999) ArticleTitleEffects of salt stress on the ultrastructure of chloroplast and the activities of some protective enzymes in leaves of sweet potato Acta Phytophysiol. Sinica 25 229–233 Occurrence Handle1:CAS:528:DyaK1MXnslSisrk%3D

    CAS  Google Scholar 

  • M. Leul W.J. Zhou (1999) ArticleTitleAlleviation of waterlogging damage in winter rape by uniconazole application: effects on enzyme activity, lipid peroxidation and membrane integrity J. Plant Growth Regul. 18 9–14 Occurrence Handle10467014 Occurrence Handle1:CAS:528:DyaK1MXlslCqu7s%3D Occurrence Handle10.1007/PL00007046

    Article  PubMed  CAS  Google Scholar 

  • D. Levy (1992) ArticleTitleThe response of potatoes (Solanum tuberosum L.) to salinity: plant growth and tuber yields in the arid desert of Israel Ann. Appl. Biol. 120 547–555 Occurrence Handle10.1111/j.1744-7348.1992.tb04914.x

    Article  Google Scholar 

  • H.Z. Li W.J. Zhou Z.J. Zhang H.H. Gu Y. Takeuchi K. Yoneyama (2005a) ArticleTitleEffect of γ-radiation on developmentyield and quality of microtubers in vitro in Solanum tuberosum L Biol. Plant. 49 625–628 Occurrence Handle1:CAS:528:DC%2BD28XlvFegsg%3D%3D Occurrence Handle10.1046/j.0028-646X.2001.00277.x

    Article  CAS  Google Scholar 

  • Li H.Z., Ye Q.F., Zhang Z.J., Zhang G.Q., Song W.J. and Zhou W.J. 2005b. Effect of salinity on chlorophyll contents, proline accumulation and antioxidant enzyme activities of plantlets in vitro in potato. Agric. Food Sci., submitted.

  • C.C. Lin C.H. Kao (1996) ArticleTitleProline accumulation is associated with inhibition of rice seedling root growth caused by NaCl Plant Sci. 114 121–128 Occurrence Handle1:CAS:528:DyaK28XhtlWlsbk%3D Occurrence Handle10.1016/0168-9452(96)04323-3

    Article  CAS  Google Scholar 

  • E.V. Maas G.J. Hoffman (1977) ArticleTitleCrop salt tolerance J. Irrig. Drain Div. 103 115–134

    Google Scholar 

  • C.A. Martinez M. Maestri E.R.G. Lani (1996) ArticleTitleIn vitro salt tolerance and proline accumulation in Andean potato (Solanum spp.) differing in frost resistance Plant Sci. 116 177–184 Occurrence Handle1:CAS:528:DyaK28XivFOrsL8%3D Occurrence Handle10.1016/0168-9452(96)04374-9

    Article  CAS  Google Scholar 

  • D.A. Meloni M.A. Oliva C.A. Martinez J. Cambraia (2003) ArticleTitlePhotosynthesis and activity of superoxide dismutaseperoxidase and glutathione reductase in cotton under salt stress Environ. Exp. Bot. 49 69–76 Occurrence Handle1:CAS:528:DC%2BD38XpsFars7Y%3D Occurrence Handle10.1016/S0098-8472(02)00058-8

    Article  CAS  Google Scholar 

  • F.Y. Men M.Y. Liu (1995) Physiology of Potato China Agriculture Press Beijing 317–335

    Google Scholar 

  • T. Murashige F. Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassay with tobacco tissue cultures Physiol. Plant. 15 473–497 Occurrence Handle1:CAS:528:DyaF3sXksFKm Occurrence Handle10.1111/j.1399-3054.1962.tb08052.x

    Article  CAS  Google Scholar 

  • A. Nadler B. Heuer (1995) ArticleTitleEffect of saline irrigation and water deficit on tuber quality Potato Res. 38 119–123 Occurrence Handle10.1007/BF02358078

    Article  Google Scholar 

  • Y. Nemoto T. Sasakuma (2002) ArticleTitleDifferential stress responses of early salt-stress responding genes in common wheat Phytochemistry 61 129–133 Occurrence Handle12169305 Occurrence Handle1:CAS:528:DC%2BD38XmtVSlsLo%3D Occurrence Handle10.1016/S0031-9422(02)00228-5

    Article  PubMed  CAS  Google Scholar 

  • H.Y. Nie W.K. Huang Y.Z. Tang J.Z. Fu (1987) Vitamin and Its Analytical Methods Shanghai Scientific and Technologic Literature Press Shanghai 234–236

    Google Scholar 

  • K. Rios-Gonzalez L. Erdei S.H. Lips (2002) ArticleTitleThe activity of antioxidant enzymes in maize and sunflower seedlings as affected by salinity and different nitrogen sources Plant Sci. 162 923–930 Occurrence Handle1:CAS:528:DC%2BD38XksVSnsL8%3D Occurrence Handle10.1016/S0168-9452(02)00040-7

    Article  CAS  Google Scholar 

  • C.B. Roy M. Vivekanandan (1998) ArticleTitleRole of aminolevulinic acid in improving biomass production in Vigna catjung, V. mungo and V. radiata Biol. Plant. 41 211–215 Occurrence Handle1:CAS:528:DyaK1cXotVGksLo%3D Occurrence Handle10.1023/A:1001806429035

    Article  CAS  Google Scholar 

  • R.K. Sairam G.C. Srivastava (2002) ArticleTitleChanges in antioxidant activity in sub-cellular fractions of tolerant and susceptible wheat genotypes in response to long-term salt stress Plant Sci. 162 897–904 Occurrence Handle1:CAS:528:DC%2BD38XksVSns7Y%3D Occurrence Handle10.1016/S0168-9452(02)00037-7

    Article  CAS  Google Scholar 

  • J.V.B. Silva W.C. Otoni C.A. Martinez L.M. Dias M.A.P. Silva (2001) ArticleTitleMicrotuberization of Andean potato species (Solanum spp.) as affected by salinity Sci. Horti. 89 91–101 Occurrence Handle10.1016/S0304-4238(00)00226-0

    Article  Google Scholar 

  • N. Smirnoff (2000) ArticleTitleAscorbic acid: metabolism and functions of a multi-facetted molecule Curr. Opin. Plant Biol. 3 229–235 Occurrence Handle10837263 Occurrence Handle1:CAS:528:DC%2BD3cXktVOnsrc%3D

    PubMed  CAS  Google Scholar 

  • InstitutionalAuthorNameSPSS (1999) SPSS for Windows, version 10.0 SPSS Inc. Chicago, 233S, Wacker Drive, Chicago, Illinois, USA

    Google Scholar 

  • J. Veramendi L. Willmitzer R.N. Trethewey (1999) ArticleTitleIn vitro grown potato microtubers are a suitable system for the study of primary carbohydrate metabolism Plant Physiol. Biochem. 37 693–697 Occurrence Handle1:CAS:528:DyaK1MXms1ahsLo%3D Occurrence Handle10.2307/2441560

    Article  CAS  Google Scholar 

  • D. Vreugdenhil P.C. Struik (1989) ArticleTitleAn integrated view of the hormonal regulation of tuber formation in potato (Solanum tuberosum L.) Physiol. Plant. 75 525–531 Occurrence Handle1:CAS:528:DyaL1MXktVChsr8%3D Occurrence Handle10.1111/j.1399-3054.1989.tb05619.x

    Article  CAS  Google Scholar 

  • D. Vreugdenhil Y. Boogaard R.G.F. Visser S.M. Bruijn ParticleDe (1998) ArticleTitleComparison of tuber and shoot formation from in vitro cultured potato explants Plant Cell Tis. Org. Cult. 53 197–204 Occurrence Handle1:CAS:528:DyaK1cXntlSru70%3D Occurrence Handle10.1023/A:1006019208758

    Article  CAS  Google Scholar 

  • K. Watanabe T. Tanaka Y. Hotta H. Kuramochi Y. Takeuchi (2000) ArticleTitleImproving salt tolerance of cotton seedlings with 5-aminolevulinic acid Plant Growth Regul. 32 99–103 Occurrence Handle1:CAS:528:DC%2BD3cXotFemt7k%3D Occurrence Handle10.1023/A:1006369404273

    Article  CAS  Google Scholar 

  • J.D. Weinstein S.I. Beale (1985) ArticleTitleEnzymatic conversion of glutamate to δ-aminolevulinic acid in soluble extracts of the unicellular green algaChlorella vulgaris Arch. Biochem. Biophys. 239 454–464 Occurrence Handle10.1016/0003-9861(85)90299-1

    Article  Google Scholar 

  • Y. Xia S. Lin H.B. Tao F.S. Zhang H.J. Hu (2000) ArticleTitleResearch on genotype difference of growth and mineral nutrition balance of wheat under NaCl stress Plant Nutr. Fert. Sci. 6 417–423

    Google Scholar 

  • X.Z. Zhang (1992) Methodology of Crop Physiology Agriculture Press Beijing, China 142–212

    Google Scholar 

  • Z.J. Zhang H.Z. Li H.L. Yao W.J. Zhou (2004) ArticleTitleEffect of paclobutrazol on explant growth and tuberization in potato J. Zhejiang Univ. (Agric. & Life Sci.) 30 318–322 Occurrence Handle1:CAS:528:DC%2BD2cXhtVKgsL%2FM

    CAS  Google Scholar 

  • Z.J. Zhang B.Z. Mao H.Z. Li W.J. Zhou Y. Takeuchi K. Yoneyama (2005a) ArticleTitleEffect of salinity on physiological characteristics, yield and quality of microtubers in vitro in potato Acta Physiol. Plant. 27 481–489 Occurrence Handle1:CAS:528:DC%2BD2MXhtlWgs7zF

    CAS  Google Scholar 

  • Z.J. Zhang W.J. Zhou H.Z. Li (2005b) ArticleTitleThe role of GAIAA and BAP in the regulation of in vitro shoot growth and microtuberization in potato Acta Physiol. Plant. 27 363–369 Occurrence Handle1:CAS:528:DC%2BD2MXps1CktL4%3D

    CAS  Google Scholar 

  • Z. J. Zhang W.J. Zhou H.Z. Li G.Q. Zhang K. Subrahmaniyan J.Q. Yu (2006) ArticleTitleEffect of jasmonic acid on in vitro explant growth and microtuberization in potato Biol. Plant. 50 453–456 Occurrence Handle10.1007/s10535-006-0069-2 Occurrence Handle1:CAS:528:DC%2BD28XntFals7w%3D

    Article  CAS  Google Scholar 

  • W.J. Zhou M. Leul (1998) ArticleTitleUniconazole-induced alleviation of freezing injury in relation to change in hormonal balanceenzyme activities and lipid peroxidation in winter rape Plant Growth Regul. 26 41–47 Occurrence Handle1:CAS:528:DyaK1cXntlemt74%3D Occurrence Handle10.1023/A:1006004921265

    Article  CAS  Google Scholar 

  • X.G. Zhu Q.D. Zhang T.Y. Kuang (2002) ArticleTitleDamage to photosynthetic functions of wheat by NaCl results mainly from its ionic effect Chinese Bull. Bot. 17 360–365 Occurrence Handle10.1111/j.1469-8137.1967.tb05999.x

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Environment and Safety Engineering, North University of China, Taiyuan, 030051, China

    Z. J. Zhang & H. Z. Li

  2. Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310029, China

    Z. J. Zhang, H. Z. Li & W. J. Zhou

  3. Center for Research on Wild Plants, Utsunomiya University, Utsunomiya, 321-8505, Japan

    Y. Takeuchi & K. Yoneyama

Authors
  1. Z. J. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Z. Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. W. J. Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Y. Takeuchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. K. Yoneyama
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to W. J. Zhou.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, Z.J., Li, H.Z., Zhou, W.J. et al. Effect of 5-Aminolevulinic Acid on Development and Salt Tolerance of Potato (Solanum tuberosum L.) Microtubers in vitro . Plant Growth Regul 49, 27–34 (2006). https://doi.org/10.1007/s10725-006-0011-9

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10725-006-0011-9

Keywords

Search

Navigation