Abstract
Two gramineous species among wild plants, Echinochloa oryzicola Vasing and Setaria viridis (L.) Beauv., and Oryza sativa L. cv. Nipponbare were subjected to salt stress. The relative growth rate (RGR), Na content, photosynthetic rate, antioxidant enzymes activity (superoxide disumutase (SOD), catalase (CAT), ascorbate peroxidase (APx) and glutathione reductase (GR)), and malondialdehyde (MDA) content in leaves after NaCl treatment were studied. RGR significantly decreased in O. sativa more than in E. oryzicola and S. viridis. Comparatively salt-tolerant S. viridis showed higher growth rate, lower Na accumulation rate in leaves, higher photosynthetic rate, and induced more SOD, CAT, APx, and GR activity and lower increase of MDA content as compared to the salt-sensitive O. sativa. At the same time, the comparatively salt-tolerant E. oryzicola also showed higher growth rate, much lower Na accumulation and no observable increase of MDA content, even though the CAT and APx activities were not induced by salinity. These results suggested that the scavenging system induced by H2O2-mediated oxidative damage might, at least in part, play an important role in the mechanism of salt tolerance against cell toxicity of NaCl in some gramineous plants
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References
Abei H. 1984. Catalase in vitro. Method in enzymology 105: 121–126.
Alscher R.G., Donahue J.L. and Cramer C.L. 1997. Reactive oxygen species and antioxidants: relationships in green cells. Physiol. Plant. 100: 224–233.
Asada K. and Takahashi M. 1987. Production and scavenging of active oxygen in photosynthesis. In: Kyle D.J., Osmond C.B. and Arntzen C.J. <nt>(eds)</nt>, Photoinhibition. Elsevier, Amsterdam, pp.227–287.
Beyer W.F. and Fridovich I. 1987. Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal. Biochem. 161: 559–566.
Dhindsa R.S. and Matowe W. 1981. Drought tolerance in two mosses: correlated with enzymatic defense against lipid peroxidation. J. Exp. Bot. 32: 79–91.
Dionisio-Sese M.L. and Tobita S. 1998. Antioxidant responses of rice seedlings to salinity stress. Plant Sci. 135: 1–9.
Foyer C.H. and Halliwell B. 1976. The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta 133: 21–25.
Foyer C.H., Maud L. and Kunert K.J. 1994. Photooxidative stress in plants. Physiol. Plant. 92: 696–717.
Gossett D.R., Millhollon E.P. and Lucas M.C. 1994. Antioxiant response to NaCl stress in salt-tolerant and salt-sensitive cultivars of cotton. Crop Sci. 34: 706–714.
Halliwell B. 1987. Oxidative damage, lipid peroxidation, and antioxidant protection in chloroplasts. Chem. Physics. Lipids 44: 327–340.
Halliwell B. and Foyer C.H. 1978. Properties and physiological function of a glutathione reductase purified from spinach leaves by affinity chromatography. Planta 139: 9–17.
Hertwig B., Streb P. and Feierabend J. 1992. Light dependence of catalase synthesis and degradation in leaves and the in. uence of interfering stress conditions. Plant Physiol. 100: 1547–1553.
Iturbe-Ormaetxe I., Escuredo P.R., Arrese-Igor C. and Becana M. 1998. Oxidative damage in pea plants exposed to water deficit of paraquat. Plant Physiol. 116: 173–181.
Kasugai S. 1939. Study on the water culture. Jpn. J. Soil Sci. Plant Nutr. 13: 662–822 (in Japanese with German summary).
Kim Y.H., Shim I.S., Kobayashi K. and Usui K. 2000. Accumulation of amino acids and glycinebetaine by NaCl treat-ment and its relation to salt tolerance in three gramineous plants. J. Weed Sci. Tech. 45: 96–103.
Lechno S., Zamski E. and Tel-Or E. 1997. Salt stress-induced responses in cucumber plants. J. Plant. Physiol. 150: 206–211.
Lin C.C. and Kao C.H. 2000. Effect of NaCl stress on H2O2 metabolism in rice leaves. Plant Growth Regul. 30: 151–155.
Nakano Y. and Asada K. 1981. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Photochem. Photobiol. 37: 679–690.
Noble C.L. and Roger M.E. 1992. Arguments for the use of physiological criteria for improving the salt tolerance in crops. Plant and Soil 146: 99–107.
Singha S. and Choudhuri M.A. 1990. Effect of salinity (NaCl) on H2O2 mechanism in Vigna and Oryza seedlings. Biochem. Physiol. Pflanzen. 186: 69–74.
Sudhakar C., Lakshmi A. and Giridarakumar S. 2001. Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus alba L.) under NaCl salinity. Plant Sci. 161: 613–619.
Vernon D.M. and Bohnert H.J. 1992. A novel methyl transferase induced by osmotic stress in the facultative halophyte Mesembryanthemum crystallinum. EMBO 11: 2077–2085.
Wise R.R. and Naylor A.W. 1987. Chilling-enhanced photo-oxidation: evidence for the role of singlet oxygen and endogenous antioxidants. Plant Physiol. 83: 278–282.
Yeo A.R. and Flowers T.J. 1983. Varietal differences in the toxicity of sodium ions in rice leaves. Physiol. Plant. 59: 189–195.
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Kim, Y., Arihara, J., Nakayama, T. et al. Antioxidative responses and their relation to salt tolerance in Echinochloa oryzicola Vasing and Setaria virdis (L.) Beauv.. Plant Growth Regulation 44, 87–92 (2004). https://doi.org/10.1007/s10725-004-2746-5
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DOI: https://doi.org/10.1007/s10725-004-2746-5