Abstract
Phototropic stimulation of dark-grown hypocotyls of Arabidopsis thaliana increased a growth inhibitor in the wild-type but not in the non-phototropic nph3-101 mutant. From light-grown wild-type shoots the inhibitor was isolated and identified as indole-3-acetonitrile (IAN) from its 1H NMR spectrum. The content of endogenous IAN in the hypocotyls of wild-type and mutant unilaterally exposed to blue light was determined using a physicochemical assay. The IAN concentration (28 μM) in the phototropically stimulated wild-type hypocotyls was about three times larger than in the dark control. However, its content in the mutant hypocotyls did not change. IAN inhibited the hypocotyl growth of the nph3-101 to the same extent as in the wild-type at concentrations higher than 10 μM. These results suggest that IAN plays a role in the phototropism of Arabidopsis thaliana hypocotyls.
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Hasegawa, T., Yamada, K., Shigemori, H. et al. Isolation and identification of blue light-induced growth inhibitor from light-grown Arabidopsis shoots. Plant Growth Regulation 44, 81–86 (2004). https://doi.org/10.1007/s10725-004-2603-6
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DOI: https://doi.org/10.1007/s10725-004-2603-6