Influence of inorganic microelements on the production of camptothecin with suspension cultures of Camptotheca acuminata

Abstract

The effects of eight microelements (I⁻, BO₃ ³⁻, MoO₄ ²⁻, Co²⁺, Cu²⁺, Mn²⁺, Fe²⁺, Zn²⁺) on the biosynthesis of camptothecin and the growth of suspension cultures of Camptotheca acuminata were studied. The increase of I⁻ to 25 μM l⁻¹, Cu²⁺ to 1 μM l⁻¹, Co²⁺ to 2 μM l⁻¹ and MoO₄ ²⁻ to 10 μM l⁻¹ in Murashige and Skoog (MS) medium resulted in 1.66, 2.84, 2.53 and 2.04 times higher of camptothecin yield than that in standard MS medium respectively. Combined treatment of I⁻ (25 μM l⁻¹), Cu²⁺ (1 μM l⁻¹), Co²⁺ (2 μM l⁻¹) and MoO₄ ²⁻ (10 μM l⁻¹) lead to improve cell dry weight, camptothecin content, and camptothecin yield to 30.56 g l⁻¹, 0.0299%, and 9.15 mg l⁻¹, respectively, which were 20.2, 208.9 and 273.8% increment respectively when compared with those of control.