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Seed longevity studies in wild type, cultivated and inter-specific recombinant inbred lines (RILs) of soybean [Glycine max (L.) Merr.]

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Abstract

Loss of seed viability is a serious hurdle in production and ambient seed storage of soybean. Understanding the factors affecting seed viability, and identification of soybean genotype(s) with higher viability is critical for higher soybean production. In this study, seeds of 125 soybean genotypes of three different species [Glycine tomentella Hayata, Glycine max subsp. soja (Siebold & Zucc.) H. Ohashi, and Glycine max(L.) Merr.] and 25 RILs (Glycine soja × Glycine max) were tested for germination immediately after harvest followed by 1, 2 and 3 years of ambient storage. Fresh seeds of all the genotypes recorded 78–99% germination with a mean of 94.02%. However, the mean value of per cent germination of all the genotypes after 1, 2 and 3 years of ambient storage decreased and found to stand at 79.51%, 52.24% and 29.18%, respectively. Among the genotypes tested, G. tomentella accession registered highest seed storability followed by G. soja, RILs and G. max. After 3 years of storage, 14 wild type genotypes and 3 RILs found to maintain > 70% germination and, were identified as ‘good storers’. Genetic divergence studies via k-mean clustering and principal component analysis grouped all genotypes in three clusters. Seed viability was significantly and negatively correlated with electrical conductivity. Good storers found to be having slow imbibition rate than poor storers in initial hours of imbibition. The good storing genotypes identified in this study will serve as an elite soybean genetic resource in developing the soybean cultivars with better seed longevity.

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Acknowledgements

First author is grateful to the UGC, New Delhi for Junior Research Fellowship (JRF) during his doctoral study and authors are grateful to the Collaborative Outstation Research Centre-Sipani Krishi Anusandhan Farm (SKAF), Mandsour, MP for providing the seed materials of the wild soybean accessions. The funding from the National Agricultural Science Fund (NASF), Indian Council of Agricultural Research, New Delhi is duly acknowledged.

Funding

Authors are grateful to the National Agricultural Science Fund, ICAR, New Delhi for funding the program.

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Authors and Affiliations

  1. Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India

    Subhash Chandra, Akshay Talukdar, Yashpal Taak, Raju R. Yadav & Manisha Saini

  2. ICAR-Indian Institute of Soybean Research, Indore, Madhya Pradesh, India

    Subhash Chandra

  3. CORC-Sipani Krishi Anusandhan Farm, Mandsaur, Madhya Pradesh, India

    N. S. Sipani

Authors
  1. Subhash Chandra
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  2. Akshay Talukdar
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  3. Yashpal Taak
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  4. Raju R. Yadav
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  5. Manisha Saini
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  6. N. S. Sipani
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Contributions

Conceptualization of research: AT, SC; Designing of the experiments: AT, SC; Contribution of experimental materials: AT, NSS; Execution of lab experiments and data collection: SC, RRY, YT, MS; Analysis of data and interpretation: SC, YT; Preparation of the manuscript: SC, AT.

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Correspondence to Akshay Talukdar.

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Chandra, S., Talukdar, A., Taak, Y. et al. Seed longevity studies in wild type, cultivated and inter-specific recombinant inbred lines (RILs) of soybean [Glycine max (L.) Merr.]. Genet Resour Crop Evol 69, 399–409 (2022). https://doi.org/10.1007/s10722-021-01240-2

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