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Exploring the Serbian GenBank barley (Hordeum vulgare L. subsp. vulgare) collection for powdery mildew resistance

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Abstract

Unlocking resistance genes in genbank collections are of prime importance for securing sustainable crop production. In this regard, the Serbian GenBank barley collection, comprising 93 local landraces and 36 commercial cultivars and elite barley breeding lines, was screened for novel resistances to powdery mildew (Blumeria graminis f. sp. hordei) using a set of 28 isolates with a wide spectrum of virulences/avirulences. No line was resistant to all the isolates, but one and three accessions showed resistance to 27 and 26 isolates, respectively. Twenty landraces (21.51 %) and ten cultivars (27.78 %) exhibited resistance to 50 % of the isolates. Infection type 2 was most frequent among resistant accessions. Nine B. graminis isolates were sufficient for gene postulation in 73 barley lines. In total, thirty-five different resistance spectra were recorded and the following known resistance genes were postulated namely, Mlra, Mlh, Mla12, Mla7(Mlu), Mlg, MlLa, Mla6, Mla7, Mlt, Mla22, Mlat, Mla1, Mlk. The majority of resistance profiles was constituted by only one line. Unidentified genes alone or in combination were proposed for twenty landraces and six cultivars. This report demonstrated that the barley collection held at the Serbian GenBank could be exploited as a new source for powdery mildew resistance.

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Acknowledgments

This study was funded by the German Academic Exchange Service (DAAD) and was conducted at the Julius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Institute for Plant Protection in Field Crops and Grassland, Kleinmachnow, Germany. This work was supported by the Ministry of Education and Science of the Republic of Serbia (Grant No. TR31092).

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Authors and Affiliations

  1. Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11000, Belgrade, Serbia

    Gordana Šurlan-Momirović & Gordana Branković

  2. Federal Research Centre for Cultivated Plants, Institute for Plant Protection in Field Crops and Grassland, Julius Kühn-Institut (JKI), 14532, Kleinmachnow, Germany

    Kerstin Flath

  3. Department of Ecology, Plant and Animal Biology, University of Coruña, 15071, A Coruña, Spain

    Cristina Silvar

  4. Faculty of Agriculture, University of Priština, Zubin Potok, Serbia

    Desimir Knežević

  5. Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Julius Kühn-Institute (JKI), Erwin-Baur-Str. 27, 06484, Quedlinburg, Germany

    Doris Kopahnke, Edgar Schliephake, Frank Ordon & Dragan Perović

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  1. Gordana Šurlan-Momirović
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  2. Kerstin Flath
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  3. Cristina Silvar
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  9. Dragan Perović
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Correspondence to Dragan Perović.

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10722_2015_246_MOESM1_ESM.tif

Hierachical clustering of the infection types for the mildew isolates in a set of 30 differential barley lines. (TIFF 7297 kb)

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Šurlan-Momirović, G., Flath, K., Silvar, C. et al. Exploring the Serbian GenBank barley (Hordeum vulgare L. subsp. vulgare) collection for powdery mildew resistance. Genet Resour Crop Evol 63, 275–287 (2016). https://doi.org/10.1007/s10722-015-0246-2

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