Abstract
Anchote (Coccinia abyssinica) is plant endemic in Ethiopia with a high calcium content grown for its edible tuberous roots. In spite of its importance as food security crop, there is no information available on molecular genetic diversity of this crop. Therefore, the aim of this study was to assess genetic diversity within and among 12 populations of anchote using ISSR markers. Using nine ISSR primers, a total of 87 scorable bands was generated of which 74 were polymorphic. Within population diversity based on polymorphic bands ranged from 13.8 to 43.53 % with a mean of 33.05 %, Nei’s genetic diversity of 0.04–0.156 with a mean of 0.12, Shannon information index of 0.07–0.23 with a mean of 0.175 and analysis of molecular variation (AMOVA) of 51.4 % were detected. With all diversity parameters, the highest diversity was obtained from Gimbi, Bedele and Ale populations, whilst the lowest was from Manna. AMOVA showed a 48.56 % between populations variability and significantly lower than that of within population variation. Population differentiation with FST was 48.56 %. From Jaccard’s pairwise similarity coefficient, Decha and Nedjo were most related populations exhibiting 0.76 similarity and Manna and Nedjo were the most distantly related populations with similarity of 0.52. The only pentanucliotide primer used in the study, Primer 880 (GGAGA)3, showed a unique band in some individuals that appeared to be associated with morphological quantitative traits (lowest seed number, high protein content, largest fruit size and smallest vine length). Illubabor and Gimbi populations exhibited highest genetic diversity so that the populations should be considered as the primary sites in designing conservation areas for this crop.
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References
Abebe D, Hagos E (1991) Plants as a primary source of drugs in the traditional health practices of Ethiopia. In: Engles JMM, Hawkes JG, Worede M (eds) Plant genetic resources of Ethiopia. Cambridge University Press, Cambridge, pp 101–113
Asfaw Z (1997) Conservation and use of traditional vegetables in Ethiopia. In: Guarino L (ed) Proceedings of the IPGRI International Workshop on Genetic Resources of Traditional Vegetables in Africa. ICRAF-HQ, Nairobi, Kenya
Bekele E (2007) Study on actual situation of medicinal plants in Ethiopia. Paper prepared for JAICAF (Japan Association for International Collaboration of Agriculture and Forestry)
Bhagyawant SS, Srivastava N (2008) Genetic fingerprinting of chickpea (Cicer arietinum L.) germplasm using ISSR markers and their relationships. Afr J Biotechnol 7(24):4428–4431
Birmeta G, Nybom H, Bekele E (2004) Distinction between wild and cultivated enset (Ensete ventricosum) gene pools in Ethiopia using RAPD markers. Hereditas 140:139–148
Da-Wei X, Xue-Jun GE, Gang H, Chang-Qin Z (2004) High genetic diversity in a rare. Narrowly Endem Primrose Species Primula Interjacens ISSR Anal 46(10):1163–1169
Excoffier L, Laval G and Schneider S (2006) Arlequin Ver 3.01, An Integrated Software Package for Population Genetics Data Analysis, Computational and Molecular Population Genetics Lab (CMPG), Institute of Zoology, University of Berne, Switzerland, http://cmpg.unibe.ch/software/arlequin3
Fekadu D (2011) Phenotypic and nutritional characterization of Anchote (Coccinia abyssinica (Lam.) Cogn.) accessions of Ethiopia. MSc thesis, Jimma Uiversity, Ethiopia
Fekadu H (2011) Effects of traditional processing methods on nutritional composition and ant-nutriotional factors of Anchote (Coccinia abyssinica (Lam.) Cogn.) grown in western Ethiopia. MSc thesis, Addis Ababa University
Fufa H, Urga K (1997) Nutritional and ant-nutritional characteristics ofAnchote (Coccinia abyssinica). Ethiop J Health Dev 11(2):163–168
Gelmesa D (2010) Shifting to alternative food source: potential to overcome Ethiopias’ malnutrition and poverty problems. ISDA, Montpellier
Getahun A (1973) Developmental anatomy of tubers of Anchote: a potential dryland tuber crop. Acta Hort 33:51–64
Hammer O, Harper D, Ryan P (2001) PAST: paleontological statistics softwarefor education and data analysis. Paleontología Electrónica 4:1–9
Hamrick JI, Godt MJ (1996) Effects of life history traits on genetic diversity in plant species. Biol Sci 351:1291–1298
Hollingsworth PM, Ennos RA (2004) Neighbour joining trees, dominant markers and population genetic structure. Heredity 92:490–498
Holstein N, Renner SS (2011) A dated phylogeny and collection records reveal repeated biome shifts in the African genus Coccinia (Cucurbitaceae). BMC J Evol Biol 11:28
Hora H (1995) Anchote: An endemic tuber crop. Jimma College of Agriculture, Jimma, Ethiopia
Hu YP, Xie XL, Wang L, Yang J, Zhang HG, Li Y (2009) An effective low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (Polygonaceae). Afr J Biotechnol 8(12):2691–2694
Jaccard P (1908) Nouvelles researches sur la distribution florale. Bull Soc Vaud Sci Nat 44:223–270
Jeffrey C (1995). Cucurbitaceae, In: Edwards S, Tadesse M and Hedberg I (eds) Flora of Ethiopia and Eritrea 2(2):52–55. National Herbarium, Addis Ababa University and Uppsala University, Sweden
Li J, Jin Z (2007) Genetic variation and differentiation in Torreya jackii Chun, an endangered plant endemic to China. Plant Sci 172:1048–1053
Li FG, Xia NH (2005) Population structure and genetic diversity of an endangered species, Glyptostrobus pensilis (Cupressaceae). Bot Bull Acad Sci 46:155–162
Lu Y, Zhang X, Pu J, Qi Y, Xie Y (2011) Molecular assessment of genetic identity and genetic stability in banana cultivars (Musa spp.) from China using ISSR markers. AJCS 5(1):25–31
Luan S, Chiang TY, Gong X (2006) High genetic diversity vs. low genetic differentiation in Nouelia insignis(Asteraceae), a narrowly distributed and endemic species in China, revealed by ISSR fingerprinting. Ann Bot 98:583–589
Mengesha D, Belew D, Gebreselassie W, Sori W (2012) Growth and yield performance of anchote [Coccinia abyssinica (Lam.) Cogn.] in response to contrasting environment. Asian J Plant Sci 11:172–181
Moulin MM, Rodrigues R, Simões L, Gonçalves A, Sudré CP, Pereira MG (2012) A comparison of RAPD and ISSR markers reveals genetic diversityamong sweet potato landraces (Ipomoea batatas (L.) Lam.). Maringá 34(2):139–147
National Research Council (1991) Managing global genetic resources: the U.S. National Plant Germplasm System, Committee on Managing Global Genetic Resources: Agricultural Imperatives, Board on Agriculture, National Research Council. National Academy Press, Washington, DC
Negash A, Tsegaye A, Treuren RV, Visser B (2002) AFLP analysis of enset clonal diversity in South and Southwestern Ethiopia for conservation. Crop Sci 42:1105–1111
Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci 70:3321–3323
Ni X, Huang Y, Wu L, Zhou R, Deng S, Wu D, Wang B, Su G, Tang T, Shi S (2006) Genetic diversity of the endangered Chinese endemic herb Primulina tabacum (Gesneriaceae) revealed by amplified fragment length polymorphism (AFLP). Genetica 127:177–183
Nybom H (2004) Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Mol Ecol 13:1143–1155
Pavlicek A, Hrda S, Flegr J (1999) Free tree software program for construction of phylogenetic trees on the basis of distance data and bootstrap/Jack Knife analysis of the tree robustness. Application in the RAPD analysis of genus Frenkelia. Folia Biol 45:97–99
Rohlf FJ (2000) NTSYS-pc, Numerical taxonomy and multivariate analysis system, Version 2.11, Exeter software, New York
Saitou N, Nei M (1987) The neighbor joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Tafesse T (2009) The Predicaments of Amhara Migrant settlers in East Wollega Zone, Ethiopia. In: Ege S, Aspen H, Teferra B and Bekele S (eds). In: Proceedings of the 16th International Conference of Ethiopian Studies
Veasey EA, Borges A, Rosa MS, Bressan Silva JR, EA Peroni N (2008) Genetic diversity in Brazilian sweet potato (Ipomoea batatas (L.) Lam., Solanales, Convolvulaceae) landraces assessed with microsatellite markers. Genetics MolBiol 31(3):725–733
Wang X, Hou X, Zhang Y, Yang R, Feng S, Li Y, Ren Y (2012) Genetic diversity of the endemic and medicinally important plant Rheum officinale as revealed by inter-simpe sequence repeat (ISSR) markers. Int J Mol Sci 13:3900–3915
Xiao M, Li Q, Wang L, Guo L, Li J, Tang L, Chen F (2006) ISSR analysis of the genetic diversity of the endangered species Sinopodophyllym hexandrum (Royle) Ying from western Sichuan Province. China. J Integr Plant Biol 48(10):1140–1146
Yeh FC, Yang R, Boyle T (1999) Microsoft window-based freeware for population genetic analysis. University of Alberta, Canada
Zhang L, Li QJ, Li HT, Chen J, Li DZ (2006) Genetic diversity and geographic differentiation in Tacca chantrieri (Taccaceae): an autonomous selfing plant with showy floral display. Ann Bot 98(2):449–457
Zhou Y, Zhou C, Yao H, Liu Y, Tu R (2008) Application of ISSR markers in detection of genetic variation among Chinese yam (Dioscorea opposita Thunb.) cultivars. Life Sci J 5(4):6–12
Acknowledgments
The authors acknowledge the Ethiopian Health and Nutrition Research Institute (EHNRI) and the Armauer Hansen Research Institute (AHRI) for providing some materials that were not available on the local market. The authors are also greateful to Addis Ababa University Food Security Thematic Research project for financial support, Debre Zeit Agricultural Research Center and Mr. Desta Fekadu for providing seeds of anchote, Dr. Amare Gessesse for making some laboratory facilities accessible for our research.
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Bekele, A., Feyissa, T. & Tesfaye, K. Genetic diversity of anchote (Coccinia abyssinica (Lam.) Cogn.) from Ethiopia as revealed by ISSR markers. Genet Resour Crop Evol 61, 707–719 (2014). https://doi.org/10.1007/s10722-014-0090-9
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DOI: https://doi.org/10.1007/s10722-014-0090-9