Skip to main content
SpringerLink
Log in

Recovery, identification and relationships by microsatellite analysis of ancient grapevine cultivars from Castilla-La Mancha: the largest wine growing region in the world

  • Research Article
  • Published:
Genetic Resources and Crop Evolution Aims and scope Submit manuscript

Abstract

A total of 374 grapevine accessions collected during prospecting works in different wine regions of Castilla-La Mancha in the period 2004–2011, in addition to 55 accessions belonging to national and international cultivars, conserved in the grapevine variety collection of Castilla-La Mancha were studied. For genetic characterization of all accessions, 26 nuclear and 5 chloroplast microsatellite markers were analyzed, yielding 158 different genetic profiles. Of the profiles, 44 were recorded as “new genotypes”, not previously described in the literature consulted. Some of them belonged to traditional cultivars cited as being present in the region in the early twentieth century, such as Churriago, Mizancho, Tortozona Tinta, Maquias and Zurieles and whose head was lost, while others were nameless cultivars hidden in ancient vineyards and unknown to science. The 26 microsatellite loci used reflected a high discrimination power (99.998 %) and a low probability that two randomly chosen individuals had identical genotypes, the probability of identity (1.02 × 10−22) was comparable to those reported for other grapevine germplasms. Four chlorotypes, A, B, C and D, were defined among the studied cultivars, most of the cultivars (53 %) carried chlorotype A, followed by chlorotypes D (28 %), C (11 %) and B (7 %). Moreover, the 158 single genotypes were analyzed for possible parent-offspring relationships. A total of nine putative parentages not previously published were obtained, and another seven were confirmed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Alonso Herrera G (1513) Agricultura General. Imprenta Real, Madrid, 1818 (translated from the original version published in 1513), Tomo I, pp 544

  • Arroyo-García R, Lefort F, De Andrés MT, Ibáñez J, Borrego J (2002) Chloroplast microsatellite polymorphisms in Vitis species. Genome 45:1142–1149

    Article  PubMed  Google Scholar 

  • Arroyo-García R, Ruiz-García L, Bolling L, Ocete R, López MA, Arnold C, Ergul A, Söylemezoglu G, Uzun HI, Cabello F, Ibáñez J, Aradhya MK, Atanassov A, Atanassov I, Balint S, Cenis JL, Costantini L, Goris-Lavets S, Grando MS, Klein BY, McGovern PE, Merdinoglu D, Pejic I, Pelsy F, Primikirios N, Risovannaya V, Roubelakis-Angelakis KA, Snoussi H, Sotiri P, Tamhankar S, This P, Troshin L, Malpica JM, Lefort F, Martínez-Zapater JM (2006) Multiple origins of cultivated grapevine (Vitis vinifera L. ssp. sativa) based on chloroplast DNA polymorphisms. Mol Ecol 15:3707–3714

    Article  PubMed  Google Scholar 

  • Boursiquot JM, Lacombe T, Laucou V, Julliard S, Perrin FX, Lanier N, Legrand D, Meredith C, This P (2009) Parentage of merlot and related wine grape cultivars of south-western France: discovery of the missing link. Aust J Grape Wine 15:144–155

    Article  Google Scholar 

  • Bowers JE, Meredith CP (1997) The parentage of a classic wine grape, cabernet sauvignon. Nat Genet 16:84–87

    Article  CAS  PubMed  Google Scholar 

  • Bowers J, Boursiquot JM, This P, Chu K, Johansson H, Meredith C (1999) Historical genetics: the parentage of Chardonnay, Gamay, and other wine grapes of north-eastern France. Science 285(5433):1562–1565

    Article  CAS  PubMed  Google Scholar 

  • Cabello F, Gallego JF, Muñoz G, Ortiz JM (1994) Malvar y Airén dos variedades presentes en la Comunidad de Madrid. Viticultura y Enología Profesional 32:25–35

    Google Scholar 

  • Cabello F, Ortiz JM, Muñoz-Organero G, Rodríguez-Torres I, Benito A, Rubio de Miguel C, García-Muñoz S, Sáiz R (2012) Variedades de Vid en España. Editorial Agrícola Española, Madrid, p 511

    Google Scholar 

  • Cabezas JA, Ibáñez J, Lijavetzky D, Vélez D, Bravo G, Rodríguez V, Carreño I, Jermakow AM, Carreño J, Ruiz-García L, Thomas MR, Martínez-Zapater JM (2011) A 48 SNP set for grapevine cultivar identification. BMC Plant Biol 11:153

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Chomé PM, Sotés V, Benayas F, Cayuela M, Hernández M, Cabello F, Ortiz J, Rodríguez-Torres I, Chaves J (2003) Variedades de Vid. Registro de Variedades Comerciales. Ed. Ministerio de Agricultura, Pesca y Alimetación. Madrid p 303

  • Crespan M, Milani N (2001) The Muscats: a molecular analysis of synonyms, homonyms and genetic relationships within a large family of grapevine cultivars. Vitis 40(4):204–208

    CAS  Google Scholar 

  • Crespan M, Meneghetti S, Cancellier S (2009) Identification and genetic relationship of the principal rootstocks cultivated in Italy. Am J Enol Vitic 60(3):349–356

    CAS  Google Scholar 

  • Duque MC (1992a) Caracterización ampelográfica de la variedad Verdoncho. Vitivinicultura 6:47–49

    Google Scholar 

  • Duque MC (1992b) Estudio ampelográfico de la variedad vitícola Moravia. Vitivinicultura 2:58–60

    Google Scholar 

  • Fernández Martínez S (1963) La Vid y el Vino en La Mancha. Ministerio de Agricultura. Dirección General de Agricultura, Sección de Investigación, Demostración y Enseñanza. Estación de Viticultura y Enología de Alcázar de San Juan y Campo de Demostración Agrícola de Valdepeñas. Madrid, pp 37–68

  • Fernández-González M, Mena A, Izquierdo P, Martínez J (2007a) Genetic characterization of grapevine (Vitis vinifera L.) cultivars from Castilla-La Mancha (Spain) using microsatellite markers. Vitis 46:126–131

    Google Scholar 

  • Fernández-González M, Martínez J, Mena A (2007b) Morphological and molecular characterization of grapevine accessions known as Moravia/o (Vitis vinifera L.). Am J Enol Vitic 58:544–547

    Google Scholar 

  • Fernández-González M, Mena A, Martínez J (2012) Identification and relationships of grapevine cultivars authorized for cultivation in Castilla-La Mancha (Spain). Am J Enol Vitic 63(4):564–567

    Article  Google Scholar 

  • Garcés J, Ortiz J, Casanova J, Gogorcena Y (2001) Caracterización de variedades de vid de la zona de Somontano (Huesca) en peligro de extinción. IV Congreso Ibérico de Ciencias Hortícolas, Cáceres

    Google Scholar 

  • García de los Salmones N (1914) Memoria general de las sesiones del congreso y ponencias presentadas. Congreso nacional de viticultura, Pamplona. Imprenta Provincial, pp 512–534

  • García E, Chacón JL, Martínez J, Izquierdo PM (2003a) Changes in volatile compounds during ripening in grapes of Airén, Macabeo and Chardonnay white varieties grow in La Mancha region (Spain). Food Sci Technol Int 9:33–39

    Article  Google Scholar 

  • García E, Chacón JL, Martínez J, Izquierdo PM (2003b) Comparación de los compuestos volátiles de las variedades Pardillo con los de otras dos variedades cultivadas en la comarca de La Manchuela. Alimentaria 340:129–137

    Google Scholar 

  • García-Muñoz S, Muñoz-Organero G, De Andrés MT, Cabello F (2011) Ampelography: an old technique with future uses: the case of minor varieties of Vitis vinifera L. from the Balearic Islands. J Inter Sci Vigne Vin 45(3):125–137

    Google Scholar 

  • García-Muñoz S, Lacombe T, De Andrés MT, Gaforio L, Muñoz-Organero G, Laucou V, This P, Cabello F (2012) Grape varieties (Vitis vinifera L.) from the Balearic Islands: genetic characterization and relationship with Iberian Peninsula and Mediterranean Basin. Genet Resour Crop Evol 59:589–605

    Article  Google Scholar 

  • Gómez-Alonso S, Fernández-González M, Mena A, Martínez J, García-Romero E (2007) Anthocyanin profile of Spanish Vitis vinifera L. red grape varieties in danger of extinction. Aust J Grape Wine 13:150–156

    Article  Google Scholar 

  • Hidalgo L (1999) Tratado de viticultura general (2ª edition). Ed. Mundi-Prensa. Madrid. 1172 pp

  • Ibánez J, Muñoz-Organero G, Zinelabidine H, De Andrés MT, Cabello F, Martínez-Zapater JM (2012) Genetic origin of the grapevine cultivar Tempranillo. Am J Enol Vitic 63(4):549–553

    Article  Google Scholar 

  • Ibáñez J, De Andrés MT, Molino A, Borrego J (2003) Genetic study of key Spanish grapevine varieties through microsatellites analysis. Am J Enol Vitic 54:22–30

    Google Scholar 

  • Ibáñez J, Vargas AM, Palancar M, Borrego J, De Andrés MT (2009) Genetic Relationship among table-grape varieties. Am J Enol Vitic 60(1):35–42

    Google Scholar 

  • Jiménez-Cantizano A, Martínez-Zapater JM, García de Luján A, Arroyo-García R (2006) Caracterización molecular de accesiones de vid del banco de germoplasma del Rancho de la Merced. Actas del XXIX Congreso Mundial de la Viña y el Vino, Logroño

    Google Scholar 

  • Lacombe T, Boursiquot JM, Laucou V, Dechesne F, Varès D, This P (2007) Relationships and genetic diversity within the accessions related to Malvasia held in the Domaine de Vassal grape germplasm repository. Am J Enol Vitic 58(1):124–131

    Google Scholar 

  • Lacombe T, Boursiquot JM, Laucou V, Di Vecchi-Staraz M, Péros JP, This P (2013) Large-scale parentage analysis in an extended set of grapevine cultivars (Vitis vinifera L.). Theor Appl Genet 126:401–414

    Article  PubMed  Google Scholar 

  • Lopes MS, Sefc KM, Eiras Dias E, Steinkellner H, da Câmara Laimer, Machado M, Da Câmara Machado A (1999) The use of microsatellites for germplasm management in a Portuguese grapevine collection. Theor Appl Genet 99:733–739

    Article  CAS  PubMed  Google Scholar 

  • MAGRAMA (2012) Ministerio de Agricultura, Alimentación y Medio Ambiente. Estadísticas Agrarias: Agricultura http://www.magrama.gob.es/es/estadistica/temas/estadisticas-agrarias/agricultura/ encuestas-de-vinedo/Accessed 23 Dec 2012

  • Martín JP, Borrego J, Cabello F, Ortiz JM (2003) Characterization of Spanish grapevine cultivar diversity using sequence-tagged microsatellite site markers. Genome 46(1):10–18

    Article  PubMed  Google Scholar 

  • Meredith CP, Bowers JE, Riaz S, Handley V, Bandman EB, Dangl GS (1999) The identity and parentage of the variety known in California as Petite Sirah. Am J Enol Vitic 50:236–242

    Google Scholar 

  • Muñoz-Organero G, Borrego J, Martín JP, Rodríguez-Torres I, Ibáñez J, Cabello F (2002) Tinto de la Pámpana Blanca y Tinto Velasco. Dos variedades de vid emparentadas. Viticultura y Enología Profesional 82:15–25

    Google Scholar 

  • Nakaune R, Nakano M (2006) Efficient methods for sample processing and cDNA synthesis by RT-PCR for the detection of grapevine viruses and viroids. J Virol Methods 134:244–249

    Article  CAS  PubMed  Google Scholar 

  • Nei M, Tajima F, Tateno Y (1983) Accuracy of estimated phylogenetic trees from molecular data. J Mol Evol 19:153–170

    Article  CAS  PubMed  Google Scholar 

  • OIV (2009) International organisation of vine and wine, 2009. OIV descriptor list for grape varietites and Vitis species, 2nd edn. OIV, Paris

    Google Scholar 

  • Ortiz JM, Santiago JL, Martínez MC, Pinto-Carnide O, Leal F, Martín JP (2003) Utilización de los microsatélites en la caracterización de variedades portuguesas de vid (Vitis vinifera L.). Sinonimias con variedades españolas. Actas de Horticultura Nº39. X Congreso Nacional de Ciencias Hortícolas. Pontevedra (Galicia), España. Viticultura 473–475

  • Page RDM (1996) Treeview: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358

    CAS  PubMed  Google Scholar 

  • Piqueras J (2005). La filoxera en España y su difusión espacial: 1878–1926. Geogr. Books, Valencia 77: 101–136

  • Roxas Clemente S (1807) Ensayo sobre las variedades de la vid común que vegetan en Andalucía. Poulet, 420 pp

  • Sánchez-Escribano EM, Martín JP, Carreño J, Cenis JL (1999) Use of sequence-tagged microsatellite site markers for characterizing table grape cultivars. Genome 42:87–93

    Article  Google Scholar 

  • Santana JC, Hidalgo E, De Lucas AI (2008) Identification and relationships of accesions grown in the grapevine (Vitis vinifera L.) germplasm bank of Castilla y León (Spain) and the varieties authorized in the VQPRD areas of the region by SSR-marker analysis. Genet Resour Crop Evol 55:573–583

    Article  Google Scholar 

  • Santana JC, Heuertz M, Arranz C, Rubio JA, Martínez-Zapater JM, Hidalgo E (2010) Genetic structure, origins, and relationships of grapevine cultivars from the Castilian Plateau of Spain. Am J Enol Vitic 61(2):214–224

    Google Scholar 

  • Sefc KM, Regner F, Glössl J, Steinkellner H (1998) Genotyping of grapevine and rootstock cultivars using microsatellite markers. Vitis 37(1):15–20

    Google Scholar 

  • Sefc KM, Lopes MS, Lefort F, Botta R, Roubelakis-Angelakis KA, Ibáñez J, Pejic I, Wagner HW, Glössl J, Steinkellner H (2000) Microsatellite variability in grapevine cultivars from different European regions and evaluation of assignment testing to assess the geographic origin of cultivars. Theor Appl Genet 100:498–505

    Article  Google Scholar 

  • Tessier C, David J, This P, Boursiquot JM, Charrier A (1999) Optimization of the choice of molecular markers for varietal identification in Vitis vinifera L. Theor Appl Genet 89:171–177

    Article  Google Scholar 

  • This P, Jung A, Boccacci P, Borrego J, Botta R, Costantini L, Crespan M, Dangl GS, Eisenheld C, Ferreira-Monteiro F, Grando S, Ibánez J, Lacombe T, Laucou V, Magalhaes R, Meredith CP, Milani N, Peterlunger E, Regner F, Zulini L, Maul E (2004) Development of a standard set of microsatellite reference alleles for identification of grape cultivars. Theor Appl Genet 109:1448–1458

    Article  CAS  PubMed  Google Scholar 

  • This P, Lacombe T, Thomas MR (2006) Historical origins and genetics diversity of wine grapes. Trends Genet 22:511–519

    Article  CAS  PubMed  Google Scholar 

  • Vargas AM, De Andrés MT, Borrego J, Ibáñez J (2009) Pedigrees of fifty table-grape cultivars. Am J Enol Vitic 60(4):525–532

    Google Scholar 

  • Vilanova M, De la Fuente M, Fernández-González M, Masa A (2009) Identification of new synonymies in minority grapevine cultivars from Galicia (Spain) using microsatellite analysis. Am J Enol Vitic 60(2):236–240

    Google Scholar 

  • Vouillamoz JF, Monaco A, Costantini L, Stefanini M, Scienza A, Grando MS (2007) The parentage of ‘Sangiovese’, the most important Italian wine grape. Vitis 46(1):19–22

    Google Scholar 

  • Wagner HW, Sefc KM (1999) IDENTITY 1.0. Centre for Applied Genetics, University of Agricultural Sciences Vienna, Austria

  • Yuste J, Martín JP, Rubio JA, Hidalgo E, Recio P, Santana JC, Arranz C, Ortiz JM (2006) Identification of autochthonous grapevine varieties in the germplasm collection at the ITA of ‘Castilla y León’ in Zamadueñas Station Valladolid, Spain. J Agric Res 4(1):31–36

    Google Scholar 

  • Zinelabidine LH, Haddioui A, Bravo G, Arroyo-García R, Martínez Zapater JM (2010) Genetic origins of cultivated and wild grapevines from Morocco. Am J Enol Vitic 61(1):83–90

    Google Scholar 

  • Zulini L, Russo M, Peterlunger E (2002) Genotyping wine and table grape cultivars from Apulia (Southern Italy) using microsatellite markers. Vitis 41(4):183–187

    CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by Grants PAI 06-0026 and IVICAM 06-0026-7697 from the Government of Castilla-La Mancha. The authors would like to thank Laura García Martín, Belén Huertas Nebreda and Raquel Torres Chica for their contribution to the prospecting work carried out during their stays at the IVICAM.

Author information

Authors and Affiliations

  1. Castilla-La Mancha Vine and Wine Institute (IVICAM), Ctra. Toledo-Albacete s/n 13700, Tomelloso, Ciudad Real, Spain

    A. Mena & J. Martínez

  2. Regional Institute of Scientific Applied Research (IRICA), University of Castilla-La Mancha, Edificio Marie Curie, Avda. Camilo José Cela, s/n 13071, Ciudad Real, Spain

    M. Fernández-González

  3. Albacete Scientific and Technology Park, Paseo de la Innovación, 1, 02006, Albacete, Spain

    M. Fernández-González

Authors
  1. A. Mena
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Martínez
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Fernández-González
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Fernández-González.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mena, A., Martínez, J. & Fernández-González, M. Recovery, identification and relationships by microsatellite analysis of ancient grapevine cultivars from Castilla-La Mancha: the largest wine growing region in the world. Genet Resour Crop Evol 61, 625–637 (2014). https://doi.org/10.1007/s10722-013-0064-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10722-013-0064-3

Keywords

Search

Navigation