Skip to main content
SpringerLink
Log in

Mass spectrometric fingerprints of seed protein for defining Lupinus spp. relationships

  • Research Article
  • Published:
Genetic Resources and Crop Evolution Aims and scope Submit manuscript

Abstract

The Lupinus genus consists of 200–600 species with diversified morphological and biochemical characteristics that provide scope for increasing the value of protein attributes in commercial lupin species through genetic improvement. This study characterised protein polymorphism among 33 selected lupin genotypes representing 19 species from Old World and New World using Matrix-Assisted Laser Desorption Ionization Time-Of-Flight (MALDI-TOF) mass spectrometry with the aim of developing a high throughput screen for assessing variation among samples within a genetic resource. MALDI-TOF mass spectrometry analysis recognized 630 polymorphic protein mass peaks. A total of 19 protein mass peaks were identified as species specific and 23 were recognized to be generally common (observed in more than 10 species). A set of 111 rare protein mass peaks common to 2–3 species were identified to uniquely distinguish the respective species in combination with the species specific mass peaks. Overall, the seed protein profiles showed extensive diversity among the species analysed. Phylogenic analysis based on the protein mass peaks clustered the Old World smooth and rough-seeded and New World lupin species separately, in broad agreement with clustering based on morphological characters, life history, cytogenetics, DNA polymorphism and electrophoreses of seed proteins. Two New World lupin species, L. mutabilis Lindl. and L. succulentus Douglas ex K. Koch were grouped into the smooth-seeded Old World group.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Ainouche A, Bayer RJ (1999) Phylogenetic relationships in Lupinus (Fabaceae: Papilionoideae) based on internal transcribed spacer sequences (ITS) of nuclear ribosomal DNA. Am J Bot 86(4):590–607

    Article  PubMed  CAS  Google Scholar 

  • Ainouche A, Bayer RJ (2000) Genetic evidence supports the new Anatolian lupine accession, Lupinus anatolicus, as an Old World ‘rough-seeded’ lupine (section Scabrispermae) related to L. pilosus. Folia Geobotanica 35(1):83–95

    Article  Google Scholar 

  • Ainouche A, Greinwald LC, Huon A (1996) Seed alkaloid composition of Lupinus tassilicus Maire (Fabaceae: Genisteae) and comparison with its related rough seeded lupin species. Biochem Syst Ecol 24(5):405–414

    Article  CAS  Google Scholar 

  • Ainouche A, Bayer RJ, Misset MT (2004) Molecular phylogeny, diversification and character evolution in Lupinus (Fabaceae) with special attention to Mediterranean and African lupines. Plant Syst Evol 246(3–4):211–222

    CAS  Google Scholar 

  • Barakat H (2004) Genetic fingerprinting and relationships of six soybeans (Glycine max L.) cultivars based on protein and DNA polymorphism. Int J Agric Biol 6(5):877–883

    CAS  Google Scholar 

  • Baranyi M, Greilhuber J, Swięcicki W (1996) Genome size in wild Pisum species. Theor Appl Genet 93(5):717–721

    Article  CAS  Google Scholar 

  • Cerletti P, Fumagalli A, Venturin D (1978) Protein composition of seeds of Lupinus albus. J Food Sci 43:1409–1411

    Article  CAS  Google Scholar 

  • Chen J, Lan P, Tarr A, Yan YM, Francki M, Appels R, Ma W (2007) Matrix-assisted laser desorption/ionization time-of-flight based wheat gliadin protein peaks are useful molecular markers for wheat genetic study. Rapid Commun Mass Spectrom 21:2913–2917

    Article  PubMed  CAS  Google Scholar 

  • Christopher SD (2008) Diversification of Lupinus (Leguminosae) in the western New World: derived evolution of perennial life history and colonization of montane habitats. Mol Phylogenet Evol 48(2):408–421

    Article  Google Scholar 

  • Cristofolini G (1989) A serological contribution to the systematics of the genus Lupinus (Fabaceae). Plant Syst Evol 166(3):265–278. doi:10.1007/bf00935955

    Article  Google Scholar 

  • Dervas G, Doxastakis G, Hadjisarva-Zinoviadi S, Triantafilakos N (1999) Lupin flour addition to wheat flour doughs and effect on rheological properties. Food Chem 66(1):67–73

    Article  CAS  Google Scholar 

  • Dunn DB (1984) Cytotaxonomy and distribution of New World lupin species. In: 3rd international lupin conference, La Rochelle, France. International Lupin Association, pp 67–85

  • Dunn DB, Gillett J (1966) Lupines of Canada and Alaska. Queen’s Press, Ottawa

    Google Scholar 

  • Duranti M, Consonni A, Magni C, Sessa F, Scarafoni A (2008) The major proteins of lupin seed: characterisation and molecular properties for use as functional and nutraceutical ingredients. Trends Food Sci Technol 19:624–633

    Article  CAS  Google Scholar 

  • Eastwood RJ, Drummond CS, Schifino-Wittmann MT, Hughes CE (2008) Diversity and evolutionary history of lupins—insights from new phylogenies. Paper presented at the 12th international lupin conference, Fremantle, Australia

  • FAOSTAT database (2011) FAO in http://faostat.fao.org/site/567/DesktopDefault.aspx

  • Gladstones JS (1974) Lupins of the Mediterranean region and Africa (trans: Agriculture ADo), vol technical bulletin 26. Australian Department of Agriculture, Perth, Australia

  • Gladstones JS (1984) Present situation and potential of Mediterranean/African Lupinus for crop production. Paper presented at the 3rd international lupine conference, La Rochelle, France

  • Gladstones JS (1994) A historical view of lupins in Australia. In: Proceedings of the first Australian lupin technical symposium. Department of Agriculture Western Australia, Perth, Western Australia

  • Gladstones JS (1998) Distribution, origin, taxonomy, history and importance of lupins. Lupins as crop plants: biology, production and utilization. CAB International, London

    Google Scholar 

  • Guemes-Vera N, Pena BRJ, Jimenez MC, Davila OG, Calderon DG (2008) Effective detoxification and decoloration of Lupinus mutabilis seed derivatives, and effect of these derivatives on bread quality and acceptance. J Sci Food Agric 88:1135–1143

    Article  CAS  Google Scholar 

  • Gupta S, Buirchell BJ, Cowling WA (1996) Interspecific reproductive barriers and genomic similarity among the rough-seeded Lupinus species. Plant Breeding 115(2):123–127

    Article  Google Scholar 

  • Hall RS, Thomas SJ, Johnson SK (2005) Australian sweet lupin flour addition reduces the glycaemic index of a white bread breakfast without affecting palatability in healthy human volunteers. Asia Pac J Clin Nutr 14:91–97

    PubMed  Google Scholar 

  • Heyn CC, Herrnstadt I (1977) Seed coat structure of old-world Lupinus species. Bot Notiser 130(4):427–435

    Google Scholar 

  • Islam S, Ma W, Ma J, Buirchell BJ, Appels R, Yan G (2011a) Diversity of seed protein among the Australian narrow-leafed lupin (Lupinus angustifolius L.) cultivars. Crop and Pasture Science 62(9):765–775

    Article  CAS  Google Scholar 

  • Islam S, Ma W, Yan G, Gao L, Appels R (2011b) Differential recovery of lupin proteins from the gluten matrix in lupin-wheat bread as revealed by mass spectrometry and two-dimensional electrophoresis. J Agric Food Chem 59(12):6696–6704

    Article  PubMed  CAS  Google Scholar 

  • Islam S, Yan G, Appels R, Ma W (2012) Comparative proteome analysis of seed storage and allergenic proteins among four narrow-leafed lupin cultivars. Food Chem. Published online: http://dx.doi.org/10.1016/j.foodchem.2012.05.081)

  • Kass E, Wink M (1997) Molecular phylogeny and phylogeography of Lupinus (Leguminosae) inferred from nucleotide sequences of the rbcL gene and ITS 1 + 2 regions of rDNA. Plant Syst Evol 208(3):139–167

    Article  Google Scholar 

  • Kussmann M, Nordhoff E, Rahbek NH, Haebel S, Rossel LM, Jakobsen L, Gobom J, Mirgorodskaya E, Kroll KA, Palm L, Roepstorff P (1997) MALDI-MS sample preparation techniques designed for various peptide and protein analytes. J Mass Spectrom 32:593–601

    Article  CAS  Google Scholar 

  • Lee YP, Mori TA, Sipsas S, Barden A, Puddey IB, Burke V, Hall RS, Hodgson JM (2006) Lupin-enriched bread increases satiety and reduces energy intake acutely. Am J Clin Nutr 84:975–980

    PubMed  CAS  Google Scholar 

  • Lee YP, Mori TA, Puddey IB, Sipsas S, Ackland TR, Beilin LJ, Hodgson JM (2009) Effects of lupin kernel flour-enriched bread on blood pressure: a controlled intervention study 1–3. Am J Clin Nutr 89:1–7

    Google Scholar 

  • Liu L, Wang A, Appels R, Ma J, Xia X, Lan P, He Z, Bekes F, Yan Y, Ma W (2009) A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding. J Cereal Sci 50:295–301

    Article  CAS  Google Scholar 

  • Lou X, van Dongen JLJ, Meijer EW (2010) Generation of CsI cluster ions for mass calibration in matrix-assisted laser desorption/ionization mass spectrometry. J Am Soc Mass Spectrom 21(7):1223–1226

    Article  PubMed  CAS  Google Scholar 

  • Magni C, Scarafoni A, Herndl A, Sessa F, Prinsi B, Espen L, Duranti M (2007) Combined 2D electrophoretic approaches for the study of white lupin mature seed storage proteome. Phytochemistry 68:997–1007

    Article  PubMed  CAS  Google Scholar 

  • Martins JM, Riottot M, de Abreu MC, Viegas-Crespo AM, Lanca MJ, Almeida JA, JoB Freire, OlP Bento (2005) Cholesterol-lowering effects of dietary blue lupin (Lupinus angustifolius L.) in intact and ileorectal anastomosed pigs. J Lipid Res 46(7):1539–1547

  • Muntz K, Blattner FR, Shutov AD (2002) Legumains, a family of asparagine-specific cysteine endopeptidases involved in propolypeptide processing and protein breakdown in plants. J Plant Physiol 159:1287–1293

    Article  Google Scholar 

  • Naganowska B, Wolko B, Sliwinska E, Kaczmarek Z (2003) Nuclear DNA Content variation and species relationships in the genus Lupinus (Fabaceae). Ann Bot 92(3):349–355. doi:10.1093/aob/mcg145

    Article  PubMed  CAS  Google Scholar 

  • Nowacki E (1963) Inheritance and biosynthesis of alkaloids in lupin. Genetica Polonica 4:161–202

    Google Scholar 

  • Pazy B, Heyn CC, Herrnstadt I, Plitmann U (1977) Studies in populations of the Old World Lupinus species. I. Chromosomes of the east-mediterranean lupines. Isr J Bot 26:115–127

    Google Scholar 

  • Petterson DS (1998) Composition and food uses of lupins. In: Gladstones JS, Atkins C, Hamblin J (eds) Lupins as crop plants: biology, production, and utilization. CABI, Oxon

    Google Scholar 

  • Petterson DS, Sipsas S, Mackintosh JB (1997) The chemical composition and nutritive value of Australian pulses. Grains Research and Development Corporation, Canberra

    Google Scholar 

  • Plitmann U, Heyn CC, Pazy B (1980) Biological flora of Israel. Lupinus palaestinus Boiss. and L. pilosus Murr. Isr J Bot 28:108–130

  • Przybylska J, Zimniak-Przybylska Z (1995) Electrophoretic patterns of seed globulins in the Old-World Lupinus species. Genet Resour Crop Evol 42(1):69–75

    Article  Google Scholar 

  • Rayburn AL, Biradar DP, Nelson RL, McCloskey R, Yeater KM (2004) Documenting intraspecfic genome size variation in soybean. Crop Sci 44(1):261–264

    Article  CAS  Google Scholar 

  • Salmanowicz BP (1999) Seed globulins in the Old World Lupinus species: comparative study by HPLC. Genet Resour Crop Evol 46(4):409–417

    Article  Google Scholar 

  • Salmanowicz BP, Przybylska J (1994) Electrophoretic patterns of seed albumins in the Old-World Lupinus species (Fabaceae): variation in the 2S albumin class. Plant Syst Evol 192(1):67–78

    Article  CAS  Google Scholar 

  • Santos CN, Ferreira RB, Teixeira AR (1997) Seed proteins of Lupinus mutabilis. J Agric Food Chem 45:3821–3825

    Article  CAS  Google Scholar 

  • Sanz Penella JM, Collar C, Haros M (2008) Effect of wheat bran and enzyme addition on dough functional performance and phytic acid levels in bread. J Cereal Sci 48(3):715–721

    Article  CAS  Google Scholar 

  • Sirtori CR, Resta D, Brambilla F, Zacherl C, Arnoldi A (2010) The effects of various processing conditions on a protein isolate from Lupinus angustifolius. Food Chem 120:496–504

    Article  CAS  Google Scholar 

  • Swofford DL (1998) PAUP: phylogenetic analysis using parsimony, 4.0b2 edn. The Illinois Natural History Survey, Champaign

    Google Scholar 

  • Talhinhas P, Neves-Martins J, Leitao J (2003) AFLP, ISSR and RAPD markers reveal high levels of genetic diversity among Lupinus spp. Plant Breeding 122(6):507–510

    Article  CAS  Google Scholar 

  • Talhinhas P, Leitao J, Neves-Martins J (2006) Collection of Lupinus angustifolius L. germplasm and characterisation of morphological and molecular diversity. Genet Resour Crop Evol 53(3):563–578

    Article  Google Scholar 

  • Turner BL (1995) A new species of Lupinus (Fabaceae) from Oaxaca, Mexico: a shrub or tree mostly three to eight meters high. Phytologia 79:102–107

    Google Scholar 

  • Williams CA, Demissie A, Harborne JB (1983) Flavonoids as taxonomic markers in old world Lupinus species. Biochem Syst Ecol 11(3):221–231

    Article  CAS  Google Scholar 

  • Wink M, Meiner C, Witte L (1995) Patterns of quinolizidine alkaloids in 56 species of the genus Lupinus. Phytochemistry 38(1):139–153

    Article  CAS  Google Scholar 

  • Wolko B, Weeden NF (1990a) Isozyme number as an indicator of phylogeny in Lupinus. Genetica Polonica 31:179–187

    Google Scholar 

  • Wolko B, Weeden NF (1990b) Relationships among lupin species as reflected by isozyme phenotype. Genetica Polonica 31:189–197

    Google Scholar 

  • Yahata E, Funatsuki WM, Nishio Z, Tabiki T, Takata K, Yamamoto Y, Tanida M, Saruyama H (2005) Wheat cultivar-specific proteins in grain revealed by 2-DE and their application to cultivar identification of flour. Proteomics 5:3942–3953

    Article  PubMed  CAS  Google Scholar 

  • Zimniak-Przybylska Z, Przybylska J (1997) Electrophoretic seed globulin patterns in some New World Lupinus species. Genet Resour Crop Evol 44(1):57–62

    Article  Google Scholar 

Download references

Acknowledgments

The authors are grateful to Jon Clements and Nindethana Seed Service Pty. Ltd., WA, Australia for providing part of seed materials and thankful to Junhong Ma for her technical support in laboratory works.

Author information

Authors and Affiliations

  1. School of Plant Biology, Faculty of Natural and Agricultural Sciences and The UWA Institute of Agriculture, The University of Western Australia, 35 Stirling Hwy, Crawley, WA, 6009, Australia

    Shahidul Islam & Guijun Yan

  2. Department of Agriculture and Food Western Australia, 3 Baron-Hay Court, South Perth, WA, 6151, Australia

    Wujun Ma

  3. Centre for Comparative Genomics, Murdoch University, 90 South Street, Murdoch, WA, 6150, Australia

    Wujun Ma & Rudi Appels

  4. Department of Horticulture, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh

    Shahidul Islam

Authors
  1. Shahidul Islam
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wujun Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rudi Appels
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Guijun Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Guijun Yan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Islam, S., Ma, W., Appels, R. et al. Mass spectrometric fingerprints of seed protein for defining Lupinus spp. relationships. Genet Resour Crop Evol 60, 939–952 (2013). https://doi.org/10.1007/s10722-012-9890-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10722-012-9890-y

Keywords

Search

Navigation