Abstract
Erianthus arundinaceus, a wild relative of sugarcane has a number of traits including tolerance to environmental stresses desired by sugarcane breeders. The value of genetic diversity studies in providing useful information for introgression breeding and germplasm conservation is well known. A previous study reported little genetic diversity in a number of Indonesian E. arundinaceus while Indian E . arundinaceus was more diverse. A small number of Chinese E. arundinaceus was observed as being relatively diverse. However, an assessment of the genetic diversity of E. arundinaceus from the major geographical areas in the world has not been reported before. In this study, 207 E. arundinaceus accessions from China, Indonesia, India, New Guinea, Philippines and Vietnam were sampled for genetic diversity analysis using AFLP markers. Three AFLP primer combinations generated 286 markers in total, with the percentage of polymorphism observed being 99.3%. A dendrogram and PCA diagram were constructed based on the matrix of similarity analyzed with UPGMA method by NTSYS software. The results showed that: (1) a high level of diversity found within the Chinese collection compared with the Indonesian collection and (2) there was a general division amongst clones from China based on those collected from eastern versus western parts of the country, and clones from Yunnan province (western China) were the most diverse.
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Acknowledgments
This work was supported by the Australian Centre for International Agricultural Research (ACIAR project code CS1/2000/038); the Yunnan Provincial Science and Technology Department (YPST project code 2006C0013Z and 2009CI059). We would like to thank L. Ma, J. Mao and Y. Bi at YSRI (Yunnan Sugarcane Research Institute) People’s Republic of China, for their assistance with the leaf materials collecting and figures colored. Thanks also to J.C. Li at CSIRO for her assistance with the molecular experiments, and to Dr Raymond Schnell (USDA, Miami) and Dr William Burnquist (Copersucar) for allowing access to the germplasm collections to collect leaf material.
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Cai, Q., Aitken, K.S., Fan, Y.H. et al. Assessment of the genetic diversity in a collection of Erianthus arundinaceus . Genet Resour Crop Evol 59, 1483–1491 (2012). https://doi.org/10.1007/s10722-011-9776-4
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DOI: https://doi.org/10.1007/s10722-011-9776-4