Abstract
This study evaluated P. edulis f. edulis intraspecific variation at DNA level using AFLPs and SSRs. About 60 specimens were collected from Colombian commercial plantations in the departments of Antioquia, Boyacá, Cundinamarca, Huila, Quindío and Tolima. Ten specimens were also included from Corpoica’s La Selva research center Passiflora germplasm bank. A non-commercial purple passion fruit plant and three species from the Passiflora genus (Passiflora maliformis, Passiflora edulis f. flavicarpa and Passiflora ligularis) were included as controls. The six most informative ones were selected from the initial screening of 49 different AFLP primer combinations; 52–92 DNA fragments for each combination in each genotype (total 419 fragments) were obtained by using these six combinations. 17 SSR primers reported in P. edulis f. flavicarpa and Passiflora alata were tested on purple passion fruit. Eight of them were successfully amplified but none showed polymorphism. Relationships among individuals were assessed on AFLP data using cluster analysis by Dice coefficient, UPGMA algorithm and the multiple correspondence analysis ordination method based on Greenacre measurement. The similarity coefficients ranged from 0.75 to 1.00 giving an average of 0.96 within the different purple passion fruit materials, showing low genetic variability for the purple passion fruit material used in this study.
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Acknowledgments
The authors wish to express their gratitude to the Colombian Ministry of Agriculture and Rural Development (2007L7592-9) and Asohofrucol (IV1351), Colombia, for funding a project entitled, “Establishing commercial purple passion fruit varieties’ genetic diversity,” and to Corpoica’s Research Center germplasm bank for the accessions sent for this research. The authors also wish to acknowledge the two anonymous referees for their fruitful comments which helped to improve the quality of the document.
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Ortiz, D.C., Bohórquez, A., Duque, M.C. et al. Evaluating purple passion fruit (Passiflora edulis Sims f. edulis) genetic variability in individuals from commercial plantations in Colombia. Genet Resour Crop Evol 59, 1089–1099 (2012). https://doi.org/10.1007/s10722-011-9745-y
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DOI: https://doi.org/10.1007/s10722-011-9745-y