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C-banded karyotype of Thinopyrum bessarabicum and identification of its chromosomes in wheat background

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Abstract

C-banded pattern in two accessions of Thinopyrum bessarabicum (Save ex Rayss) A. Löve (2n = 2x = 14, EbEb) and their idiogram was established. C-banding analysis was further used to identify the chromosomes of Tritipyrum amphiploid (2n = 6x = 42, AABBEbEb) and a BC1F2 genotype from wheat and Tritipyrum. Two 18S-26S rDNA loci were detected on Th. bessarabicum chromosomes by in situ hybridization using an 18S-26S rDNA probe. Eb chromosomes in Tritipyrum generally were identified by their distinctive C-banding patterns which reflected heterochromatin regions. C-banding procedure resulted in sharp and distinct bands in one or both ends of Eb chromosomes without interval bands. Observed C-bands in Eb genome mainly reflected the telomeric and subtelomeric sequences which also showed more strong signals in genomic in situ hybridization. Results showed the importance of the C-banding technique as a screening tool in identification of addition and substitution lines in the progenies of wheat and Tritipyrum crosses during segregating generations.

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Acknowledgments

The authors thank Professor Bikram S. Gill and Professor Bernd Friebe from Kansas State University, USA for providing the pTA71 clone and Dr. Goerge Fedak from AgriFood, Ottawa, Canada for providing Th. bessarabicum seeds. The first author gratefully acknowledges Dr. Michael Francki from Murdoch University, Australia for his advices on in situ hybridization.

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Authors and Affiliations

  1. Agronomy and Plant Breeding Department, Faculty of Agriculture, University of Kurdistan, P. O. Box 416, Sanandaj, Iran

    G. Mirzaghaderi

  2. Plant Breeding Department, Faculty of Agriculture, University of Shahid Bahonar, Kerman, Iran

    H. Shahsevand Hassani

  3. Plant Breeding Department, Faculty of Agriculture, Tarbiat Modares University, P. O. Box 14115-336, Tehran, Iran

    G. Karimzadeh

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  1. G. Mirzaghaderi
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  2. H. Shahsevand Hassani
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  3. G. Karimzadeh
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Correspondence to G. Mirzaghaderi.

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Mirzaghaderi, G., Shahsevand Hassani, H. & Karimzadeh, G. C-banded karyotype of Thinopyrum bessarabicum and identification of its chromosomes in wheat background. Genet Resour Crop Evol 57, 319–324 (2010). https://doi.org/10.1007/s10722-009-9509-0

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