Abstract
A total of 82 grapevine genotypes were sampled from several areas of the Italian region of Sicily where vineyards are widely spread. The grapevines were characterized using six microsatellite markers (VVS2, VVMD5, VVMD7, VVMD27, VrZAG62 and VrZAG79) to evaluate genetic diversity. Thirty-seven of the 82 cultivars sampled had their names quoted in historical and literary sources, while 45 cultivars from old vineyards did not have their names reported in ancient literature. According to their genetic profiles at SSR loci, 70 different cultivars were found, while interesting cases of synonymies (Regina and Moscato bianco, Alicante and Dolcetta or among different clones of Zibibbo and Catarratto) and cases of homonymy (Frappato and Nerello Mascalese) were discovered. Several genetic parameters were calculated to assess the efficacy of the loci chosen in this work. Pairwise genetic distances between all cultivars were calculated. A dendrogram representing the genetic similarities among cultivars was depicted using the UPGMA method to investigate their relationships, explaining them from a historical point of view. The cluster distribution of cultivars clearly does not reflect their current geographical distribution, suggesting successive introductions of cultivars in Sicily from different areas of origin.
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References
Acerbi G (1825) Delle viti italiane o sia dei materiali per servire alla classificazione. Monografia e sinonimia precedute dal tentativo di una classificazione geografica delle viti. Silvestri Publisher, Milano
Alagna Spanò A (1873) La vite e il vino in Marsala. Giornale d’Agricoltura, Industria e Commercio del Regno d’Italia, Roma
Alleweldt G, Possingham JV (1988) Progress in grapevine breeding. Theor Appl Genet 75:669–673
Anderson B (2006) The wines of Italy. The Italian Trade Commission, New York
Aradhya MK, Dangl GS, Prins BH, Boursiquot JM, Walker AM, Meredith CP, Simon CJ (2003) Genetic structure and differentiation in cultivated grape Vitis vinifera L. Genet Res (Cambridge) 81:179–192
Arroyo-Garcìa R, Ruiz-Garcìa L, Bolling L, Ocete R, Lòpez MA, Arnold C, Ergul A, Soylemezoolu G, Uzun HI, Cabello F, Ibanez J, Aradhya MK, Atanassov A, Atanassov I, Balint S, Cenis JL, Costantini L, Gorislavets S, Grando MS, Klein BY, McGovern PE, Merdinoglu D, Pejic I, Pelsy F, Primirikos N, Rissovannaya V, Roubelakis-Angelakis KA, Snoussi H, Sotiri P, Tamhankar S, This P, Troshin L, Malpica JM, Lefort F, Martìnez-Zapater JM (2006) Multiple origins of cultivated grapevine (Vitis vinifera L. ssp. sativa) based on chloroplast DNA polymorphisms. Mol Ecol 15:3707–3714
Bica D (2007) Vitigni di Sicilia. Priulla Publisher, Palermo
Bollettino Ampelografico (1878) Primi studi ampelografici nei circondari di Catania e di Acireale. IX–X. Eredi Botta Publishers, Roma
Bollettino Ampelografico (1883) Catalogo dei vitigni coltivati nella provincia di Palermo. XVI. Eredi Botta Publishers, Roma
Botta R, Scott NS, Eynard I, Thomas MR (1995) Evaluation of microsatellite sequence—tagged site markers for characterization Vitis vinifera cultivars. Vitis 2:99–102
Bowers JE, Meredith CP (1997) The parentage of a classic wine grape, Cabernet Sauvignon. Nat Genet 16:84–87
Bowers JE, Dangl GS, Vignani R, Meredith CP (1996) Isolation and characterization of new polymorphic simple sequence repeat loci in grape (Vitis vinifera L.). Genome 39:628–633
Bowers JE, Boursiquot JM, This P, Chu K, Johansson H, Meredith CP (1999) Historical genetics: the parentage of Chardonnay, Gamay, and other wine grapes of Northeastern France. Science 285:1562–1565
Collesano G (1998) Evoluzione varietale della viticoltura da vino in Sicilia. Vignevini 25(7–8):27–32
Costantini L (1989) Plant exploitation at Grotta dell’Uzzo, Sicily: new evidence for the transition from Mesolithic to Neolithic subsistence in southern Europe. In: Harris DR, Hillman GC (eds) Foraging and farming: the evolution of the plant exploitation. Unwin & Hyman, London, pp 197–206
Costantini L, Monaco A, Vouillamoz JF, Forlani M, Grando MS (2005) Genetic relationships among local Vitis vinifera cultivars from Campania. Vitis 44:25–34
Cupani F (1696) Hortus Catholicus seù illustrissimi, & excellentissimi principis Catholicae. Ed Benzi, Neapoli
Cupani F (1697) Supplemen alterum ad Hortum Catholicum. Gramignani Publisher, Panormi
Dangl GS, Mendum ML, Prins BH, Walker MA, Meredith CP, Simon CJ (2001) Simple sequence repeat analysis of a clonally propagated species: a tool for managing a grape germplasm collection. Genome 44:432–438
Doyle JJ, Doyle LJ (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Fazello T (1558) De rebus siculis decades duae. Mayda and Carra Publishers, Palermo
Franks T, Botta R, Thomas MR (2002) Chimerism in grapevines: implications for cultivar identity, ancestry and genetic improvement. Theor Appl Genet 104:192–199
Gagliano F (2008) I vitigni autoctoni siciliani. In: Viticultura ed enologia di Sicilia. Dalla ricerca in vigna e in cantina alle strategie di marketing. Azienda Grafica Editoriale snc, Catania
Galet P (1979) A practical ampelography: grapevine identification. University Press, Ithaca
Galet P (1991) Prècis d’ampèlographie pratique. 6th edn. Charles Déhan Publishers, Montpellier
Gallo A (1595) Dieci giornate della vera agricoltura. In: Le vicende tecniche ed economiche della viticoltura e dell’enologia in Italia. Ed Arti Grafiche Guardoni, 1937
Geremia G (1834) Vertunno Etneo ovvero Stafulegrafia, storia delle varietà delle uve che trovasi nel d’intorno dell’Etna. Atti dell’Accademia Gioenia, Catania 10:10–14
Geremia G (1835) Vertunno Etneo ovvero Stafulegrafia, storia delle varietà delle uve che trovasi nel d’intorno dell’Etna. Atti dell’Accademia Gioenia, Catania 10:201–221
Geremia G (1836) Vertunno Etneo ovvero Stafulegrafia, storia delle varietà delle uve che trovasi nel d’intorno dell’Etna. Atti dell’Accademia Gioenia, Catania 11:313–340
Geremia G (1839) Vertunno Etneo ovvero Stafulegrafia, storia delle varietà delle uve che trovasi nel d’intorno dell’Etna. Atti dell’Accademia Gioenia, Catania 14:3–55 and 57–68
Hammer K, Laghetti G (2006) Small agricultural islands and plant genetic resources. IGV-CNR Publishers, Bari, 244 pp
Hokanson SC, Szewc-McFadden AK, Lamboy WF, McFerson JR (1998) Microsatellite (SSR) markers reveal genetic identities, genetic diversity, and relationships in a Malus × domestica Borkh. core subset collection. Theor Appl Genet 97:671–683
IPGRI, UPOV, OIV (1997) Descripteurs de la vigne (Vitis spp.). Union internationale pour la protection des obtentions végetales. Genève, Suisse/Office international de la Vigne et du Vin, Paris, France/Institut international des ressources phytogènè tiques, Rome
Kirst M, Cordeiro CM, Rezende GDSP, Grattapaglia D (2005) Power of microsatellite markers for fingerprinting and parentage analysis in Eucalyptus grandis breeding populations. J Hered 96:161–166
Leighton R (1993) Morgantina Studies, Volume IV: The protohistoric settlement on the Cittadella. Princeton University Press, Princeton
Leighton R (1999) Sicily before history: an archaeological survey from the palaeolithic to the iron age. Duckworth Publisher, London
Levadoux L (1956) Les populations sauvages et cultivées de Vitis vinifera L. Ann Amelior Plantes 6:59–118
Lopes MS, Sefc M, Eiras Dias E, Steinkellner H, Laimer da Camara Machado M, da Camara Machado A (1999) The use of microsatellites for germplasm management in a Portuguese grapevine collection. Theor Appl Genet 99:733–739
Mendola A (1868) Trattato del Catalogo della collezione di viti italiane e straniere radunate in Favara. Parrino and Carini Publishers, Favara
Minà Palumbo F (1853) Sistema ampelografico per la classificazione dei vitigni siciliani. Giorn Agr Pastorizia, Palermo 2:163–188
Minà Palumbo F (1891) Viticoltura Sicula. Cenni storici—Atti Acc Zelanti Acireale 3:12
Morgante M, Olivieri AM (1993) PCR-amplified microsatellites as markers in plant genetics. Plant J 3:175–182
Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Nat Acad Sci USA 70(12):3321–3323
Nicosia F (1735) Il podere fruttifero e dilettevole. Felicella Publisher, Palermo
Paetkau D, Calvert W, Stirling I, Stroberk C (1995) Microsatellite analysis of population structure in Canadian polar bears. Mol Ecol 4:347–354
Pastena (1971) I catarratti bianchi. Atti Accademia Vite e Vino, p. 23
Riaz S, Garrison KE, Dangl GS, Boursiquot JM, Meredith CP (2002) Genetic divergence and chimerism within ancient asexually propagated winegrape cultivars. J Am Soc Hortic Sci 127:508–514
Rohlf FJ (1998) Numerical taxonomy and multivariate analysis system ver. 2.02. Applied Biostatics Inc., New York
Scott KD, Eggler P, Seaton G, Rossetto M, Ablett EM, Lee LS, Henry RJ (2000) Analysis of SSRs derived from grape ESTs. Theor Appl Genet 100:723–726
Sefc M, Regner F, Glossl J, Steinkellner H (1998a) Genotyping of grapevine and rootstock cultivars using microsatellites markers. Vitis 37:15–20
Sefc KM, Steinkellner H, Glossl J, Kampfer S, Regner F (1998b) Reconstruction of a grapevine pedigree by microsatellite analysis. Theor Appl Genet 97:227–231
Sefc KM, Regner F, Tureschek E, Glossl J, Steinkellner H (1999) Identification of microsatellite sequences in Vitis riparia and their application for genotyping of different Vitis species. Genome 42:367–373
Sefc KM, Lopes MS, Lefort L, Botta R, Roubelakis-Angelakis KA, Ibanez J, Pejic I, Wagner H, Glössl J, Steinkellner H (2000) Microsatellite variability in grapevine cultivars from different European regions and evaluation of assignment testing to assess the geographic origin of cultivars. Theor Appl Genet 100:498–505
Sefc KM, Lefort F, Grando MS, Scott KD, Steinkellner H, Thomas MR (2001) Microsatellite markers for grapevine: a state of the art. In Roubelakis-Angelakis KA (ed) Molecular biology and biotechnology of the grapevine. Kluwer Publishers, Amsterdam, pp 433–463
Sestini D (1812a) Dei vini in generale della Sicilia. Accademia dei Gorgofili, Firenze, Manuscript 11c
Sestini D (1812b) Dei vini di Mascali della Sicilia. Accademia dei Gorgofili, Firenze, Manuscript 12c
Snoussi H, Harbi Ben Slimane M, Ruiz-Garcìa L, Martìnez-Zapater JM, Arroyo-Garcìa R (2004) Genetic relationship among cultivated and wild grapevine accessions from Tunisia. Genome 47:1211–1219
Tessier C, David J, Thip P, Boursiquot JM, Charrier A (1999) Optimization of the choice of molecular markers for varietal identification in Vitis vinifera L. Theor Appl Genet 98:171–177
Thomas MR, Scott NS (1993) Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed as sequenced-tagged sites (STSs). Theor Appl Genet 86:985–990
Thomas MR, Matsumoto S, Cain P, Scott NS (1993) Repetitive DNA of grapevine: classes present and sequences suitable for cultivar identification. Theor Appl Genet 86:173–180
Tollefsrud MM, Sønstebø JH, Brochmann C, Johnsen Ø, Skrøppa T, Vendramin GG (2009) Combined analysis of nuclear and mitochondrial markers provide new insight into the genetic structure of North European Picea abies. Heredity 102:549–562
Venuti A (1516) Notensis De agricoltura opusculum. Sigismondo Mayr Alemano Publisher, Napoli
Wagner HW, Sefc KM (1999) IDENTITY 1.0. Centre for Applied Genetics, University of Agricultural Sciences, Vienna
Weber JL (1990) Informativeness of human (dC-dA)n-(dG-dT)n polymorphisms. Genomics 7:524–530
Acknowledgments
The authors are grateful to Associazione Italiana Agricoltua Biologica (AIAB) and Legambiente for financial support. The authors are also grateful to the Regione Siciliana, Assessorato Agricoltura e Foreste, for financial support through the project ‘Conservazione in vivo ed in vitro di varieta minori di fruttiferi in via di estinzione, tipici degli ambienti mediterranei’. The authors wish to thank Mr. Vincenzo Marino for his technical assistance.
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Carimi, F., Mercati, F., Abbate, L. et al. Microsatellite analyses for evaluation of genetic diversity among Sicilian grapevine cultivars. Genet Resour Crop Evol 57, 703–719 (2010). https://doi.org/10.1007/s10722-009-9506-3
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DOI: https://doi.org/10.1007/s10722-009-9506-3