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Development and utilization of diagnostic DAMD-PCR markers for Capsicum accessions

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Abstract

A polymerase chain reaction (PCR) based approach involving the directed amplification of minisatellite DNA region (DAMD-PCR) was used to identify accession specific DNA markers and study genetic relationships between and within 15 accessions corresponding to 11 species in genus Capsicum. A touch down PCR profile and unique chemical concentration of ingredients resulted in reproducible and reliable DNA amplifications. The number of amplified products varied from 1 to 12 fragments depending on the template DNA and the primers. The DAMD-PCR technique provided a total of 38 accession specific DNA markers (diagnostic DAMD-PCR) which can be utilized in accession identification, preservation and genetic studies of Capsicum germplasm. Based on 1,292 polymorphic and monomorphic DNA markers directed with 22 minisatellite specific primers, accessions were divided into four major groups, three of which corresponded to the three distinct Capsicum complexes. Capsicum chacoense was found to be the most distinct species.

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Acknowledgement

This research was in part supported by the Scientific Research Projects Administration Unit of Akdeniz University.

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Authors and Affiliations

  1. Department of Field Crops, Faculty of Agriculture, Akdeniz University, 07059, Antalya, Turkey

    Ayşe Gul Ince, Mehmet Karaca & A. Naci Onus

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  1. Ayşe Gul Ince
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  2. Mehmet Karaca
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  3. A. Naci Onus
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Correspondence to Mehmet Karaca.

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Ince, A.G., Karaca, M. & Onus, A.N. Development and utilization of diagnostic DAMD-PCR markers for Capsicum accessions. Genet Resour Crop Evol 56, 211–221 (2009). https://doi.org/10.1007/s10722-008-9356-4

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  • DOI: https://doi.org/10.1007/s10722-008-9356-4

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