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Genetic variability in natural populations of Paspalum dilatatum Poir. analyzed by means of morphological traits and molecular markers

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Abstract

Native species show adaptive traits that are difficult to find in introduced species. The Pampas region in Argentina is a valuable nature reserve of grasses and Paspalum dilatatum Poir. is one of the most important grasses found there. Based on ploidy level and on morphological traits, five biotypes of P. dilatatum have been described. Two of them were included in this study: a tetraploid biotype with sexual reproduction and a pentaploid biotype with apomictic reproduction. We analyzed the genetic diversity in eight native populations from the Salado basin, Argentina, using both quantitative traits and molecular data (RAPD) with these aims: to obtain information of the degree of phenotypic variation in that area, to know which the pattern of distribution of this variation is and to look for association between molecular markers with populational or biotypic differentiation. Cluster analysis based on morphological data grouped the individuals of the different populations by ploidy level. Molecular markers showed the inverse situation because individuals were grouped by geographic origin as opposed to biotype. Moreover, since RAPD did not discriminate between biotypes with sexual or apomictic reproduction, they are probably not associated with mating system. The results let us conclude that polygenic traits such as LP, LBSR, NRT and NSP can discriminate between biotypes and molecular markers such as bands 12, 40, 19 and 46 can be used to discriminate among populations, probably because they detect neutral variation.

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Acknowledgements

This study has been funded by grants from the Consejo Nacional de Investigaciones Científicas y Técnicas (1074/98) and from Universidad Nacional de La Plata. We would like to thanks to P.K.Ingvarsson for helpful comments to the manuscript and N. Di Lorenzo for the data on resistance to Claviceps paspali.

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  1. María Victoria García

    Present address: Department of Ecology and Environmental Science, University of Umeå, SE-891 87, Umeå, Sweden

Authors and Affiliations

  1. Departamento de Ciencias Biológicas, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, 60 y 119, 1900 La Plata, Buenos Aires, Argentina

    María Victoria García, Pedro A. Balatti & Miguel J. Arturi

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  1. María Victoria García
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  2. Pedro A. Balatti
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  3. Miguel J. Arturi
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Correspondence to María Victoria García.

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García, M.V., Balatti, P.A. & Arturi, M.J. Genetic variability in natural populations of Paspalum dilatatum Poir. analyzed by means of morphological traits and molecular markers. Genet Resour Crop Evol 54, 935–946 (2007). https://doi.org/10.1007/s10722-006-9147-8

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