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Comparison of genetic variation among accessions of Aegilops tauschii using AFLP and SSR markers

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Abstract

Genetic diversity of 54 accessions of Aegilops tauschii from five countries was assessed using sequence-tagged microsatellites (or simple sequence repeats, SSRs) and amplified fragment length polymorphisms (AFLPs). In the case of AFLP analysis, a total of 256 amplification products obtained, 234 of them were polymorphic across all the 54 accessions. A total of 224 fragments were obtained from the 24 SSR primers and 219 of fragments were polymorphic across all the genotypes screened. Based on both AFLP and SSR markers, the highest percentage of polymorphisms were obtained in Iranian and accessions of unknown origin. The highest polymorphic information content (PIC) value was observed for SSRs (0.82) while the highest marker index (MI) value was for AFLPs (8.5) reflecting the hyper-variability of the first and the distinctive nature of the second system. Principal co-ordinate analysis (PCO) revealed congruent patterns of genetic relationships for both data sets, but did not group accessions strictly according to their geographical origins. Poor correlation was found between AFLP and SSR marker loci. This low association may be due to low number of AFLP and SSR markers. These results show that molecular markers can help to organize the genetic variability and expose useful diversity for breeding purposes.

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References

  • Dudnikov AJ (2000) Multivariate analysis of genetic variation in Aegilops tauschii from the world germplasm collection. Genet Resour Crop Evol 47:185–190

    Article  Google Scholar 

  • Dvorak J, Luo MC, Yang ZL, Zhang HB (1998) The structure of the Aegilops tauschii genepool and the evolution of hexaploid wheat. Theor Appl Genet. 97:657–670

    Article  CAS  Google Scholar 

  • Guyomarc’h H, Sourdille P, Charmet G, Edwards KJ, Bernard M (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat. Theor Appl Genet 104:1164–1172

    Article  PubMed  CAS  Google Scholar 

  • Hammer K (1980). Vorarbeiten zur monographischen Darstellung von Wildpflanzensortimenten: Aegilops L. Kulturpflanze 28:33–180

    Article  Google Scholar 

  • Lelley T, Stachel M, Grausgruber H, Vollmann J (2000) Analysis of relationships between Aegilops tauschii and the D genome of wheat utilizing microsatellites. Genome 43:661–668

    Article  PubMed  CAS  Google Scholar 

  • Lubbers EL, Gill KS, Cox TS, Gill BS (1991) Variation of molecular markers among geographically diverse accessions of Triticum tauschii. Genome 34:354–361

    Google Scholar 

  • Naghavi MR, Amirian R (2005) Morphological characterization of accessions of Aegilops tauschii. Int J Agri Biol 7:392–394

    Google Scholar 

  • Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590

    PubMed  Google Scholar 

  • Pestsova E, Korzun V, Goncharov NP, Hammer K, Ganal MW, Röder MS (2000) Microsatellite analysis of Aegilops tauschii germplasm. Theor Appl Genet 101:100–106

    Article  CAS  Google Scholar 

  • Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breeding 2:225–238

    Article  CAS  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Rohlf FJ (1998) NTSYS-PC. Numerical taxonomy and multivariate analysis system, version 2.00, Exeter Software, Setauket, NY

    Google Scholar 

  • Saeidi H, Rahiminejad MR, Vallian S, Heslop-Harrison JS (2006) Biodiversity of diploid D-genome Aegilops tauschii Coss. in Iran measured using microsatellites. Genet Resour Crop Evol 53:1477–1484

    Article  Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, Van der Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23:4407–4414

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This research was supported by the International Foundation for Science, Stockholm, Sweden, through a grant to Dr. M. R. Naghavi. In addition, the authors wish to thank Dr. V. Mohammadi for providing the Aegilops accessions.

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Authors and Affiliations

  1. Agronomy and Plant Breeding Department, University of Tehran, Agricultural College, 31587-77871, Karaj, Iran

    Mohammad Reza Naghavi

  2. Department of Genomics, Agricultural Biotechnology Research Institute of Iran, Mahdasht Road, Karaj, Iran

    Mohsen Mardi, Seyed Mostafa Pirseyedi, Mehrbanu Kazemi, Payam Potki & Mohammad Reza Ghaffari

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  1. Mohammad Reza Naghavi
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  2. Mohsen Mardi
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  3. Seyed Mostafa Pirseyedi
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  4. Mehrbanu Kazemi
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  5. Payam Potki
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  6. Mohammad Reza Ghaffari
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Correspondence to Mohammad Reza Naghavi.

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Naghavi, M.R., Mardi, M., Pirseyedi, S.M. et al. Comparison of genetic variation among accessions of Aegilops tauschii using AFLP and SSR markers. Genet Resour Crop Evol 54, 237–240 (2007). https://doi.org/10.1007/s10722-006-9143-z

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  • DOI: https://doi.org/10.1007/s10722-006-9143-z

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