Abstract
The seed protein contents of 179 accessions belonging to ten species of genus Fagopyrum were determined by means of the Kjeldahl method. The results indicated that there are significant differences of seed protein content among different accessions within the same species. The average protein content of F. esculentum is 12.94%, ranging from 8.81–18.71%, and the protein content of Sibano is the highest, up to 18.71%, Gantian 1 the lowest with 8.81%. The average protein content of tartary buckwheat is 12.17%, with the range from 7.82–18.94%, and the protein content of cultivated tartary buckwheat Qianku 2 is the highest (18.59%), and that of Ganku 1 (7.82%) the lowest. Moreover, there are significant differences of seed protein content among different buckwheat species and between the different buckwheat types (between common buckwheat and tartary buckwheat, between the diploid and the tetraploid, between the cultivated and the wild, and between the big-achene group and the small-achene group). The average seed protein content of F. giganteum is the highest in all tested species, up to 17.81% with the range of 13.91–21.27%, and that of F. callianthum the lowest, only 8.31%.
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References
An SH, Zheng XM, Song ZC (1997) The agricultural characters and quality trait of partial tartary buckwheat native to Guizhou. Guizhou Agricult Sci 25:42–45
Campbell CG (1995) Inter-specific hybridization in the genus Fagopyrum. Curr Adv Buckwheat Res I–III, 255–263. Proceedings of the 6th International Symposium on buckwheat in Shinshu, 24–29 August 1995 (T. Matano and A. Ujihara, eds). Shinshu University Press
Campbell CG (1997) Buckwheat, Fagopyrum esculentum Moench. (Promoting conservation and use of underutilized and neglected crops, 19). Perez-Arbelaez-IPK/IPGRI, Gatersleben/Rome
Chen QF (1999a) A study of resources of Fagopyrum (Polygonaceae) native to China. Bot J Linn Soc 130:53–64
Chen QF (1999b) Hybridization between Fagopyrum (Polygonaceae) species native to China. Bot J Linn Soc 131:177–185
Chen QF (2001a) Karyotype analysis of five Fagopyrum species native to China. Guihaia 21:107–110
Chen QF, Hsam SLK, Zeller FJ (2004) A study of cytology, isozyme, and interspecific hybridization on the big-achene group of buckwheat species (Fagopyrum, Polygonaceae). Crop Sci 44:1511–1518
Guo XN, Yao HY (2005) The studies of structure, low digestibility and physiological functions on buckwheat protein. Machinery for Cereal Oil and Food Process, pp 60–64
Hammer K (2001) Polygonaceae. In: Hanelt P, Institute of Plant Genetics and Crop Plant Research (eds) Mansfeld’s Encyclopedia of Agricultural and Horticultural Crops. Springer, Berlin, pp 285–308
Hirose T, Ujihara A, Kitabayashi H, Minami M (1993) Morphology and identification by isozyme analysis of interspecific hybrids in buckwheats. Fagopyrum 13:25–30
Javornik B, Eggum BQ, Kreft I (1980) Studies on protein fractions and protein quality of buckwheat. Genetika 13:115–121
Kayashita J, Shimaoka I, Nakajoh M (1995) Hypocholesterolemic effect of buckwheat protein extract in rats fed cholesterol enriched diets. Nutr Res 15:691–698
Kayashita J, Shimaoka I, Nakajoh M, Kishida N, Kato N (1999) Consumption of a buckwheat protein extract retards 7,12-dimethylbenz [α] anthracene-induced mammary carcinogenesis in rats. Biosci Biotechnol Biochem 63:1837–1839
Krotov AS (1975) Fagopyrum Mill. Flora of cultivated plants III. Groat crops [in Russia]. Kolos Leningrad, pp 7–118
Lin RF (ed) (1994) Buckwheat native to China. China Agricultural Press, Beijing, pp 97–104 (in Chinese)
Lin RF, Zhou YN, Wang R, Li JY (eds) (2001) A study on the extract of tartary buckwheat. Toxicological Safety of the Extract of Tartary Buckwheat. Proceeding of the 8th International Symposium on buckwheat, Chunchon, Korea. Adv Buckwheat Res 602–607
Liu DS, Xu DY (1997) The analysis of protein content and amino acid composition in buckwheat. Crop Qual Resour 2:26–28
Ohnishi O (1995) Discovery of new Fagopyrum species and its implication for the studies of evolution of Fagopyrum and of the origin of cultivated buckwheat. In: Matano T, Ujihara A (eds) Proceedings of the 6th International Symposium on buckwheat in Shinshu, vol I–III, 24–29 August 1995. Shinshu University Press, Curr Adv Buckwheat Res, pp 175–190
Ohnishi O, Matsuoka Y (1996) Search for the wild ancestor of buckwheat.Taxonomy of Fagopyrum (Polygonaceae) species based on morphology, isozymes and cpDNA variability. Genes Genet Syst 71:383–390
Piao SL, Li LH (eds) (2001) The actuality of produce and exploitation of Fagopyrum in China. Proceeding of the 8th International Symposium on buckwheat, Chunchon, Korea. Adv Buckwheat Res 571–576
Radovic RS, Maksimovic Brkljacic MJ, Gasic IEV, Savic PA (1999) 2s albumin from buckwheat (Fagopyrum esculentum Moench) seeds. J Agricult Food Chem 47:1467–1470
Rout MK, Chrungoo NK (1999) The lysine and methionine rich basic subunit of buckwheat grain legumin: some results of a structural study. Biochem Mol Biol Int 47:921–926
Tang Y, Zhao G (1990) The pilot study of nutrient quality on buckwheat character resources native to Sichuan. Buckwheat newslett 2:20–24
Tomotake H, Shimaoka I, Kayashita J, Yokoyama F, Nakajoh M, Kato N (2000) A Buckwheat protein product suppresses gallstone formation and plasma cholesterol more strongly than soy protein isolate in hamsters. J Nutr 130:1670–1674
Ujihara A, Nakamura Y, Minami M (1990) Interspecific hybridization in genus Fagopyrum - properties of hybrids (F. esculentum Moench. × F. cymosum Meissner) through ovule culture, in Gamma Field Symposium, No.29. Inst. Radiation Breeding, NIAR, MAFF, Japan, pp 45–51
Yang JX (ed) (2002) The experiment technology tutorial of biochemistry and molecular biology. Scientific Publisher, Beijing, PR China, pp 25–26
Ye NJ, Guo GQ (eds) 1992. Classification, origin and evolution of genus Fagopyrum in china. In: Lin et al. (ed) Proceeding of the 5th International Symposium on buckwheat, Taiyuan, China. Chinese Agricultural Publishing House, Beijing China, pp 19–28
Zhang YZ, Chen QF (2004) The actuality and expectation on buckwheat studies. Seeds 23:39–42
Acknowledgements
We are grateful to Ms. Mao Chun (Institute of Weining Agricultural Sciences, Guizhou, PR China) and Prof. Chai Yan (Northwest A & F University, PR China) for providing some materials, to Zhang Yubin, Song Qingfa (the Guizhou Normal University) and Experimental Analysis Center (the Guizhou Normal University) for providing some experimental conditions, to Pan Shouju, Li Jianhui, Wang Tian, Zhang Yizhong, Ren Cuijuan, Wang Aiguo, Sheng Maoyin (the Institute of Plant Genetics and Breeding) for many helps, and to the Natural Science Foundation of China (30270852, 30471116), Program for New Century Excellent Talents in University (NCET-2004-0913), Guizhou Key International Cooperation Project (Qianke Hewai G Zi 2005#400108), Guizhou Oversea Talent Project (Qianren Xiangmu Zizhu Hetong 2004#02), and the key project of students in Guizhou Normal University (GNU-SP-2005-SJXY-A01) for providing funds.
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This study was financed by the Natural Science Foundation of China (30270852, 30471116), Program for New Century Excellent Talents in University (NCET-2004-0913), Guizhou Key International Cooperation Project (Qianke Hewai G Zi 2005#400108), Guizhou Oversea Talent Project (Qianren Xiangmu Zizhu Hetong 2004#02), and Key Project of Students in Guizhou Normal University (GNU-SP-2005-SJXY-A01)
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Guo, YZ., Chen, QF., Yang, LY. et al. Analyses of the seed protein contents on the cultivated and wild buckwheat Fagopyrum esculentum resources. Genet Resour Crop Evol 54, 1465–1472 (2007). https://doi.org/10.1007/s10722-006-9135-z
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DOI: https://doi.org/10.1007/s10722-006-9135-z