Abstract
To study the relationships and genetic diversity among wild hops, Humulus lupulus, we analyzed 133 samples of wild hops collected from Europe, Asia and North America using polymorphism on 11 microsatellite loci. Although only three primers showed bands in Japanese hops, all other samples showed polymorphic bands at most loci. There were no duplicate genotypes among samples of European, Chinese and North American hops, and each individual hop could be distinguished completely. The phylogenetic tree constructed from DA distance with the UPGMA method showed a large cluster comprised of European hops, although Russian hops from the Caucasus and Altai regions were separate from the European cluster. Chinese and North American samples gave distinct clusters suggesting genetic differentiation. This study has indicated that hop microsatellite DNA is differentiated, and is dependent upon the origin in regions of Europe, Asia and North America.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
H.J. Barth, C. Klinke and C. Schmidt, The Hop Atlas, the History and Geography of the Cultivated Plant. Nürnberg: Joh. Barth & Sohn (1994).
Belkhir K.P., Borsa P., Chikhi L., Raufaste N. and Bonhomme F. 2001. GENETIX 4.02, logiciel sous Windows TM pour la génétique des populations. (Available from http://www.univ-montp2.fr/˜genetix/genetix/genetix.htm).
J. Bowers, J.M. Boursiquot, P. This, K. Chu, H. Johansson and C. Meredith, Historical genetics: the parentage of Chardonnay, Gamay, and other wine grapes of Northeastern France. Science 285 (1999) 1562-1565
J.L. Brady, N.S. Scott and M.R. Thomas, DNA typing of hops (Humulus lupulus) through application of RAPD and microsatellite marker sequences converted to sequence tagged sites (STS). Euphytica 91 (1996) 277-284
J.M. Cournuet and G. Luikart, Description and power analysis of two test detecting recent population bottlenecks from allele frequency data. Genetics 144 (1996) 2001-2014
P. Darby, New selection criteria in hop breeding. In: E. Seigner (ed.) International Hop Growersȁ9 Convention. Pulawy: Proceedings of the Scientific Commission (1999) pp. 3-6
C. Ferris, R.A. King and G..M. Hewitt, Isolation within species and the history of glacial refugia. In: P.M. Hollingsworth, R.M. Bateman and R.J. Gornall (eds.) Molecular Systematics and Plant Evolution. London: Taylor Francis (1999) pp. 20-34
A.M. Hadonou, R. Walden and P. Darby, Isolation and characterization of polymorphic microsatellites for assessment of genetic variation of hops (Humulus lupulus L.). Mol. Ecol. Note 4 (2004) 280-282
J. Jakše, K. Kindlhofer and B. Javornik, Assessment of genetic variation and differentiation of hop genotypes by microsatellite and AFLP markers. Genome 44 (2001) 773-782
J. Jakše, D. Bandelj and B. Javornik, Eleven new microsatellites for hop (Humulus lupulus L.). Mol. Ecol. Note 2 (2002) 544-546
R.C. Jones, D.A. Steane, B.M. Potts and R.E. Vaillancourt, Microsatellite and morphological analysis of Eucalyptus globulus populations. Can. J. For. Res. 32 (2002) 59-66
Y. Matsuoka, Y. Vigouroux, M.M. Goodman, J.G. Sanchez, E. Buckler and J. Doebley, A single domestication for maize shown by multilocus microsatellite genotyping. Proc. Natl. Acad. Sci. USA 99 (2002) 6080-6084
M. Moir, Hops – A millennium review. J. Am. Soc. Brew. Chem. 58 (2000) 131-146
A. Murakami, Comparison of sequence of rbcL and non-coding regions of Chloroplast DNA and ITS2 region of rDNA in genus Humulus. Breed. Sci. 50 (2000) 155-160
A. Murakami, Structural differences in the intergenic spacer of 18S-26S rDNA and molecular phylogeny using partial external transcribed spacer sequence in Hop, Humulus lupulus. Breed. Sci. 51 (2001) 163-170
A. Murakami, P. Darby, B. Javornik, M.S.S. Pais, E. Seigner, A. Lutz and P. Svoboda, Molecular evolution of hops, Humulus lupulus. In: E. Seigner (ed.) International Hop Growersȁ9 Convention. Zalec-Dobrna: Proceedings of the Scientific Commission (2003) pp. 92 pp
Murakami A., Darby P., Javornik B., Pais M.S.S., Seigner E., Lutz A. and Svoboda P. 2005. Molecular Phylogeny of Hops, Humulus lupulus L. (in preparation).
M. Nei, F. Tajima and Y. Tateno, Accuracy of estimated phylogenetic trees from molecular data. J. Mol. Evol. 19 (1983) 153-170
R.A. Neve, Hops. London: Chapman and Hall (1991).
V. Rybáček, Hop Production. Amsterdam: Elsevier (1991).
E.A. Small, Numerical and nomenclatural analysis of morpho-geographic taxa of Humulus. Syst. Bot. 3 (1978) 37-76
J.F. Stevens, A.W. Taylor, G.B. Nickerson, M. Ivancic, J. Henning, A. Haunold and M.L. Deinzer, Prenylflavonoid variation in Humulus lupulus: distribution and taxonomic significance of xanthogalenol and 4′-O-methylxanthohumol. Phytochemistry 53 (2000) 759-775
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Murakami, A., Darby, P., Javornik, B. et al. Microsatellite DNA Analysis of Wild Hops, Humulus lupulus L.. Genet Resour Crop Evol 53, 1553–1562 (2006). https://doi.org/10.1007/s10722-005-7765-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10722-005-7765-1