Abstract
A PCR (polymerase chain reaction) amplification method using newly designed S-RNase primers was carried out in five Korean-bred pear cultivars and ten Japanese-bred pear cultivars. A new S-RNase allele, designated as S10, was discovered from ‘Chengsilri’, containing a 1513 bp and two exons (213 bp in total) that coded for a peptide of 71 amino acids. The S10-RNase allele contained the three conserved cysteine residues peculiar to S-RNase in Japanese pear and one histidine residue essential for RNase activity. We compared nucleotide sequence similarity of the exon regions of ten pear S-RNase alleles. The nucleotide sequence of S1 showed a high similarity to S4 (97.4%) and the new S10 shows 77.8% (S5) to 84.4% (S4) similarity with the other pear S-RNase alleles. S10 had a unique restriction endonuclease site for ‘HhaI’, with digests yielding fragments of 1235 and 491 bp. The S-genotype of pear cultivar (‘Chengsilri’) was determined to be S5S10 by PCR–RFLP (restriction fragment length polymorphism). Cluster analysis of 49 known S-RNase alleles of the Rosaceae separated into two divergent groups are as follows: group I: pear and apple, group II: almond, sweet cherry and mume.
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Abbreviations
- 2D-PAGE:
-
Two dimensional-polyacrylamide gel electrophoresis
- CTAB:
-
Cetyl-tri-methyl ammonium bromide
- HV:
-
Hypervariable
- IEF:
-
Isoelectric focusing
- PCR–RFLP:
-
Polymerase chain reaction–restriction fragment length polymorphism
- SDS:
-
Sodium dodecyl sulfate
References
Brace J., King G.J. and Ockendon D.J. (1994). Sex. Plant Reprod. 7: 203–208
Carlos C., Takasaki T., Saito T., Yoshimura Y., Norioka S. and Nakanishi T. (2001). Reconsideration of S-genotype assignments, and discovery of a new allele based on S-RNase PCR–RFLP in Japanese pear cultivars. Breed. Sci. 51: 5–11
Dellaporta S.T., Wood J. and Hicks J.B. (1983). A plant DNA minipreparation: version II. Plant Mol. Biol. Rep. 1: 19–21
Dicenta F. and García J.E. (1993). Inheritance of self-compatibility in almond. Heredity 70: 313–317
Hiratsuka S., Okada Y., Kawai Y., Tamura F. and Tanabe K. (1995). Stylar basic proteins corresponding to 5 self-incompatibility alleles of Japanese pears. J. Jpn. Soc. Horticult. Sci. 64: 471–478
Hiratsuka S., Kubo T. and Okada Y. (1998). Estimation of self-incompatibility genotype in Japanese pear cultivars by stylar protein analysis. J. Jpn. Soc. Horticult. Sci. 67: 491–496
Ishimizu T., Sato Y., Saito T., Yoshimura Y., Norioka S., Nakanishi T. and Sakiyama F. (1996). Identification and partial amino-acid sequences of seven S-RNases associated with self-incompatibility of the Japanese pearPyrus pyrifolia Nakai. J. Biochem. 120: 326–334
Ishimizu T., Shinkawa T., Sakiyama H. and Norioka S. (1998). Primary structural features of Rosaceous S-RNases associated with gametophytic self-incompatibility. Plant Mol. Biol. 37: 931–941
Ishimizu T., Inoue K., Shimonaka M., Saito T., Terai O. and Norioka S. (1999). PCR-based method for identifying the S-genotypes of Japanese pear cultivars. Theor. Appl. Genet. 98: 961–967
Kawata Y., Sakiyama F., Hayashi F. and Kyogoku Y. (1989). Eur. J. Biochem. 187: 244–262
Kim H.T., Hirata Y. and Nou I.S. (2002). Determination of S-genotype of pear (Pyrus pyrifolia) cultivars by S-RNase sequencing and PCR–RFLP analyses. Mol. Cells 13: 444–451
Matsumoto S., Kitahara K., Komori S. and Soejima J. (1999). A new S-allele in apple‘Sg’, and its similarity to the ‘Sf’ allele from ‘Fuji’. HortScience 34: 708–710
Matsumoto S. and Kitahara K. (2000). Discovery of a new self-incompatibility allele in apple. HortScience 35: 1329–1332
Norioka N., Norioka S., Ohnishi Y., Ishimizu T., Oheyama C., Nakanishi T. and Sakiyama F. (1996). Molecular cloning and nucleotide sequences of cDNAs encoding S-allele specific stylar RNases in a self-incompatible cultivar and self-compatible mutant of Japanese pear, Pyrus pyrifolia Nakai. J. Biochem. 120: 335–345
Sassa H., Hirano H. and Ikehashi H. (1993). Identification and characterization of stylar glycoproteins associated with self-incompatibility genes of Japanese pearPyrus serotina Rehd. Mol. Gen. Genet. 241: 17–25
Sawamura Y., Saito T., Shoda M., Yamamoto T., Sato Y., Hayashi T. and Kotobuki K. (2002). A new self-incompatible allele in Japanese pear ‘Shinsei’ and ‘Shinkou’. J. Jpn. Soc. Horticult. Sci. 71: 342–347
(1984). A genetic approach to the transmission of compatibility in almond. Options Méditerr Serie Études 2: 123–128
Terami H., Torikata H. and Shimazu Y. (1946). Analysis of the sterility factors existing in varieties of the Japanese pear (Pyrus serotina Rehd. var. culta Rehd.). Studies Hort. Inst. Kyoto Imp. Univ 3: 267–271
Yamamoto T., Iketani H., Nishizawa Y., Notsuka K., Hibi T., Hayashi T. and Matsuta N. (2000). Transgenic grapevine plants expressing a rice chitinase with enhanced resistance to fungal pathogenes. Plant Cell Rep. 19: 639–646
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Kim, HT., Hirata, Y., Kim, HJ. et al. The Presence of a New S-RNase Allele (S10) in Asian Pear (Pyrus pyrifolia (Burm; Nakai)). Genet Resour Crop Evol 53, 1375–1383 (2006). https://doi.org/10.1007/s10722-005-5034-y
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DOI: https://doi.org/10.1007/s10722-005-5034-y