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Identification of sweet cherry cultivars (Prunus avium L.) and analysis of their genetic relationships by chloroplast sequence-characterised amplified regions (cpSCAR)

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Abstract

Ten cpSCAR markers that show polymorphism in Prunus avium were used to fingerprint sweet cherry cultivars. The purpose of the study was also to contribute to identification and to help determine their genetic interrelationships. Samples of ‘0900 Ziraat’, a superior Turkish variety, which were collected in several locations all over Turkey, had identical cpSCAR patterns, and they resembled a common European haplotype, A. ‘Sweetheart’, ‘Summit’ and ‘Canada Giant’ and their haplotype are intermediate between the previously described haplotypes A and B, which were originally found in Central and Eastern European sweet and wild cherries, and those from Northern Turkey, respectively. The data therefore suggests a local maternal descent (within Europe and Asia Minor) of the cultivars analysed. Our results show that chloroplast DNA analysis is a straightforward way to classify cherry cultivars. We compare our results to others previously reported for sweet cherry cultivars, and conclude that cpSCAR diversity data could be considered for phylogenetic studies in this group.

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Abbreviations

AFLP:

amplified fragment length polymorphism

cpDNA:

chloroplast DNA

cpSCAR:

chloroplast DNA sequence characterised region

HA:

chloroplast haplotype A

HB:

chloroplast haplotype B

PCR–RFLP:

polymerase chain reaction–restriction fragment length polymorphism

RAPD:

random amplified polymorphic DNA

SSR:

simple sequence repeat

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Authors and Affiliations

  1. Mustafa Kemalpasa Vocational School, Uludag University, 16500, Bursa, Turkey

    Aydin Turkec

  2. Department of Molecular Biology and Genetics, Bogazici University, 34342, Bebek, Istanbul, Turkey

    Muge Sayar

  3. Federal Research Centre for Forestry, Hauptstrasse 7, A-1140, Vienna, Austria

    Berthold Heinze

Authors
  1. Aydin Turkec
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  2. Muge Sayar
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  3. Berthold Heinze
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Correspondence to Aydin Turkec.

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Turkec, A., Sayar, M. & Heinze, B. Identification of sweet cherry cultivars (Prunus avium L.) and analysis of their genetic relationships by chloroplast sequence-characterised amplified regions (cpSCAR). Genet Resour Crop Evol 53, 1635–1641 (2006). https://doi.org/10.1007/s10722-005-2285-6

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  • DOI: https://doi.org/10.1007/s10722-005-2285-6

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