Abstract
In order to formulate appropriate strategies for the conservation and utilization of the wild mulberry genetic resources available in India, a study was undertaken with 20 mulberry genotypes from the four different species. Seventeen intersimple sequence repeat primers were used to generate a total of 114 markers, of which 98 (85.96%) were polymorphic. Seven unique bands for Morus serrata Roxb. and one for both M. serrata Roxb. and Morus macroura Miq. were identified, of which one fragment has been sequenced and deposited in the EMBL-GeneBank (AJ-585512). The genetic dissimilarity coefficients varied from 0.078 to 0.530 among these genotypes and from 0.168 to 0.465 among the species. The dendrograms realized from these markers clustered the genotypes into three groups. The outermost group was M. serrata Roxb., which was followed by the group of M. macroura Miq. and the innermost group contained genotypes of Morus indica L. and Morus alba L. This intermixing of genotypes of M. indica and M. alba supports the view that M. indica is merely a synonym of M. alba. Distribution of the genotypes on a two-dimensional figure upon multidimensional scaling with ALSCAL program, further, confirmed the genetic divergence between the cultivated and wild mulberry groups. On the basis of the results a few potential wild mulberry genotypes were identified for its conservation and utilization in breeding programs to confer the stress tolerance to the cultivated varieties of mulberry.
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References
InstitutionalAuthorNameAnonymous (1962) The Wealth of India. A Dictionary of Indian Raw Materials and Industrial Products. VI (L-M) Council of Scientific and Industrial Research New Delhi 429–439
M.J. Asins E.A. Carbonell (1989) ArticleTitleDistribution of genetic variability in a durum wheat collection Theor. Appl. Genet. 77 287–294 Occurrence Handle10.1007/BF00266199
Bhattacharya E. and Ranade S.A. 2001. Molecular distinction amongst varieties of Mulberry using RAPD and DAMD profiles. BMC Plant Biology 3 (This article is available from: http:// www.biomedcentral.com /1471–2229/1/3).
B. Bornet F. Goraguer G. Joly M. Branchard (2002) ArticleTitleGenetic diversity in European and Argentinean cultivated potatoes (Solanum tuberosum subsp. tuberosum) detected by inter-simple sequence repeats (ISSRs) Genome 45 481–484 Occurrence Handle12033616 Occurrence Handle1:CAS:528:DC%2BD38Xks1yjur8%3D Occurrence Handle10.1139/g02-002
S.B. Dandin R. Kumar S. Rabindran M.S. Jolly (1987) ArticleTitleCrossability studies in mulberry Indian J. Sericult. 26 11–14
Dandin S.B., Basavaiah Kumar R. and Venkateshaiah H.V. 1993. Phytogeographical studies in genus Morus L. I. Geographical distribution and natural variation of Morus serrata Roxb. Plant Genetic Resources: developing National Policy, organized by the Indian Society of Plant Genetic Resources, New Delhi, December 1–2, 1993. pp. 11.
B.C. Das S. Krishnaswami (1965) ArticleTitleSome observations on interspecific hybridization in mulberry Indian J. Sericult. 4 1–8
A. Bustos ParticleDe C. Casanova C. Soler N. Jouve (1998) ArticleTitleRAPD variation in wild populations of four species of the genus Hordeum (Poaceae) Theor. Appl. Genet. 99 147–156
A. Dhar M. Ahsan (1989) Mulberry species and distribution in North-western Himalayas K. Sengupta S.B. Dandin (Eds) Genetic Resources of Mulberry and Utilization CSR and TI Mysore 33–35
N.K. Dwivedi N. Suryanarayana B.N. Sisheelamma A.K. Sikdar M.S. Jolly (1989) ArticleTitleInterspecific hybridisation in mulberry Sericologia 29 147–149
J. Felsenstein (1993) PHYLIP (Phylogeny inference package) version 3.5c Department of Genetics, University of Washington Seattle
M.K. Gururajan (1960) ArticleTitleVarieties of mulberry – a classification Indian Silk J. 1 12–15
H. Hirano (1977) ArticleTitleEvaluation of affinities in mulberry and its relatives by peroxidase isozyme technique JARQ 11 228–233 Occurrence Handle1:CAS:528:DyaE1cXls1Shurc%3D
H. Hirano (1982) ArticleTitleVarietal differences of leaf protein profiles in mulberry Phytochemistry 21 1513–1518 Occurrence Handle1:CAS:528:DyaL38XmtFarurY%3D Occurrence Handle10.1016/0031-9422(82)85008-5
J.D. Hooker (1885) Flora of British India. V: 491 L. Reeve and Co. Ltd., The East House Book AshfordKentUK
C.J. Jones K.J. Edward S. Castagglione M.O. Winfield F. Sale C. Wiel ParticleVan de G. Bredemeijer M. Buiatti E. Maestri A. Malcevshi N. Marmiroli R. Alert G. Volkaert J. Rueda R. Linacero A. Vazquez A. Karp (1997) ArticleTitleReproducibility of RAPD, AFLP and SSR markers in plants by network of European laboratories Mol. Breed. 3 381–390 Occurrence Handle1:CAS:528:DyaK2sXntFensbg%3D Occurrence Handle10.1023/A:1009612517139
N. Mantel (1967) ArticleTitleThe detection of disease clustering and a generalized regression approach Cancer Res. 27 175–178
M.G. Murray W.F. Thompson (1980) ArticleTitleRapid isolation of high-molecular weight plant DNA Nucleic Acid Res. 8 4321–4327 Occurrence Handle7433111 Occurrence Handle1:CAS:528:DyaL3cXmtVSmtL8%3D
N.V. Nair S. Nair T.V. Sreenivasan M. Mohan (1999) ArticleTitleAnalysis of genetic diversity and phylogeny in Saccharumrelated genera using RAPD markers Genet. Resour. Crop Evol. 46 73–79 Occurrence Handle10.1023/A:1008696808645
M. Nei W. Li (1979) ArticleTitleMathematical model for study the genetic variation in terms of restriction endonucleases Proc. Natl. Acad. Sci. USA 74 5267–5273
M. Ommachan (1976) Flora of Bhopal Angiosperms. J.K. Jain and Brothers Bhopal
R.N. Parker (1956) Forest flora for the Punjab with Hazara and Delhi EditionNumber3 Govt. Printing Press LahoreWest Pakistan 471–473
C.E. Parkinson (1923) A Forest Flora of Andaman Islands Bishen Singh and Mahindra Pal Singh Dehra Dun 155
T. Perez J. Albornoz A. Dominiquez (1998) ArticleTitleAn evaluation of RAPD fragment reproducibility and nature Mol. Ecol. 7 1347–1357 Occurrence Handle9787445 Occurrence Handle1:CAS:528:DyaK1cXmvFGitr8%3D Occurrence Handle10.1046/j.1365-294x.1998.00484.x
S. Ravindran V.A. Rao V.G. Naik A. Tikader P. Mukheerjee K. Thangavelu (1997) ArticleTitleDistribution and variations in mulberry germplasm Indian J. Plant Genet. Res. 10 233–242
L.H. Rieseberg (1996) ArticleTitleHomology among RAPD fragments in Inter-specific comparison Mol. Ecol. 5 99–105 Occurrence Handle1:CAS:528:DyaK28Xit12hurY%3D
J.M. Rodriguez T. Berke L. Engle J. Nienhuis (1999) ArticleTitleVariation among and within Capsicum species revealed by RAPD markers Theor. Appl. Genet. 99 147–156 Occurrence Handle1:CAS:528:DyaK1MXlvFGltLw%3D Occurrence Handle10.1007/s001220051219
A. Sarkar S.K. Jalaja R.K. Datta (2000) ArticleTitleGradual improvement of mulberry varieties under irrigated conditions in south India and the optimal program for varietal selection in the tropics Sericologia 40 449–461
C.R. Sastry (1984) ArticleTitleMulberry varieties, exploitation and pathology Sericologia 24 333–359
P.H.A. Sneath R.R. Sokal (1973) Numerical Taxonomy W. H. Freeman San Francisco
B.N. Susheelamma M.S. Jolly K. Giridhar K. Sengupta (1990) ArticleTitleEvaluation of germplasm genotypes for drought resistance in mulberry Sericologia 30 327–341
A. Tikader S.B. Dandin (2001) ArticleTitleBreeding behaviour of some wild mulberry Indian Silk 40 9–10
Tikader A. and Rao A. 2003. Intra and interspecific hybridisation in mulberry. Bull. Indian Acad. Sericult. (in press).
N. Tosti V. Negri (2002) ArticleTitleEfficiency of three PCR-based markers in assessing genetic variation among cowpea (Vigna unguiculata subsp. unguiculata) landraces Genome 45 268–275 Occurrence Handle11962624 Occurrence Handle1:CAS:528:DC%2BD38XjtlSns7c%3D Occurrence Handle10.1139/g01-146
Y. Tsumura K. Ohba S.H. Strauss (1996) ArticleTitleDiversity and inheritance of inter-simple repeat polymorphism in Douglas fir (Pseudotsuga menziesii) and Sugi (Cryptomeria japonica) Theor. Appl. Genet. 92 40–45 Occurrence Handle1:CAS:528:DyaK28Xlt1Cmu7k%3D Occurrence Handle10.1007/BF00222949
K. Vijayan (2003) ArticleTitleGenetic relationships of Japanese and Indian mulberry (Morus spp.) genotypes revealed by DNA fingerprinting Plant Syst. Evol. 243 221–232 Occurrence Handle10.1007/s00606-003-0078-y
K. Vijayan S.N. Chatterjee (2003) ArticleTitleISSR profiling of Indian cultivars of mulberry (Morus spp.) and its relevance to breeding programs Euphytica 131 53–63 Occurrence Handle1:CAS:528:DC%2BD3sXis1GlsLY%3D Occurrence Handle10.1023/A:1023098908110
K. Vijayan S.N. Chatterjee C.V. Nair (2005) ArticleTitleMolecular characterization of mulberry genetic resources indigenous to India Genet. Resour. Crop Evol. 52 77–86 Occurrence Handle1:CAS:528:DC%2BD2MXjt1yks7s%3D Occurrence Handle10.1007/s10722-005-0288-y
G. Watt (1891) Dictionary of Economic Products of India NumberInSeriesvol. 5 Periodical Experts Delhi 279–284
J.G.K. Williams A.R. Kubelik K.J. Livak J.A. Rafalski S.V. Tingey (1990) ArticleTitleDNA polymorphism amplified by arbitrary primers are useful as genetic markers Nucleic Acids Res. 18 6531–6535 Occurrence Handle1979162 Occurrence Handle1:CAS:528:DyaK3MXjslWmsA%3D%3D
B.R.D. Yadav T. Pavankumar (1996) ArticleTitleOccurrence of a new distributional ranges of Morus laevigata Wall. and its variants in South India Indian J. Sericult. 35 163–164
I.V. Yap R.J. Nelson (1996) WINBOOT: a program for performing bootstrap analysis for binary data to determine the confidence limits of UPGMA-based dendrograms International Rice Research Institute Philippines
F.W. Young B.N. Easterling B.N. Forsyth (1984) The general Euclidean model for scaling three mode dissimilarities: theory and application H.G. Law G.W. Snyder SuffixJr. J. Hattie R.P. McDonald (Eds) Research Methods for Multi-Node Data Analysis in the Behavioral Sciences Praeger New York
F.W. Young D..F. Harris (1990) Multidimensional scaling: procedure ALSCAL M. Norusis (Eds) SPSS Base System: User's Guide SPSS Chicago, USA 397–461
E. Zietkiewicz A. Rafalski D. Labuda (1994) ArticleTitleGenome fingerprinting by simple sequence repeat (SSR) – anchored polymerase chain reaction amplification Genomics 20 176–183 Occurrence Handle8020964 Occurrence Handle1:CAS:528:DyaK2cXmsVOhtbo%3D Occurrence Handle10.1006/geno.1994.1151
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Vijayan, K., Tikader, A., Kar, P.K. et al. Assessment of Genetic Relationships between Wild and Cultivated Mulberry (Morus) Species using PCR Based Markers. Genet Resour Crop Evol 53, 873–882 (2006). https://doi.org/10.1007/s10722-004-6148-3
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DOI: https://doi.org/10.1007/s10722-004-6148-3